[Preparation and clinical application of an intelligent determination and analysis system for glomerular filtration rate].

OBJECTIVE: To prepare an intelligent determination and analysis system for renal glomerular filtration rate(GFRBMAS), and to explore its value in clinical setting. METHODS: GFRBMAS was prepared by programming with VB 6.0 software. GFR of 79 inpatients suffering from the different diseases was determined accurately by using clearance rate of (99)Tc(m)-diethylene triamine pentaacetic acid (DTPA) (Tc-GFR). The serum creatinine (SCr), blood urea nitrogen (BUN), serum uric acid (Uric), serum calcium (Ca) and serum phosphorus (P) were determined with both GFRBMAS and 7170S automatic biochemistry determination apparatus (ititachi), and the result of GFR was compared with that determined by using GFRBMAS and 7170S automatic biochemical determination apparatus. At the same time GFR was determined by using Robert formula (GFRBMAS-GFR, Robert-GFR), and creatinine clearance rate was calculated with Cockcroft/Gault formula (CG-CCr). All the results were compared and analyzed. RESULTS: No significant difference of SCr, BUN, Uric, Ca and P values determined by two methods. Robert-GFR and CG-CCr values were significantly lower than Tc-GFR value in the normal renal function group and the renal insufficiency group (P<0.01) and that of GFRBMAS-GFR was close to that of Tc-GFR and relative analysis showed that the values of GFRBMAS-GFR, Robert-GFR, CG-CCr showed significantly positive correlation with that of Tc-GFR, but negative correlation with values of SCr and BUN (P<0.05 or P<0.01). CONCLUSION: GFRBMAS-GFR, Robert-GFR and CG-CCr could all reflect GFR with accuracy to certain extent and GFRBMAS-GFR can take the place of Tc-GFR in clinical setting.

The treatment of intrahepatic calculosis by applying helix hydro-jet lithotripsy under video choledochoscope: a report of 30 cases.

BACKGROUND AND AIMS: Intrahepatic lithiasis is a common disease in southeast Asia [Sheen-Chen and Chou, Acta Chir Scand 156:387-390, 1990], and a difficult problem of biliary surgery. There is no established method of treating patients with intrahepatic stones [Uchiyama et al., Arch Surg 137:149-533, 2002]. In recent years, resection of the affected liver lobe or segment is the best therapeutic option to completely remove the source of recurrent infection. The need for endoscopic treatment modalities is evident because hepatic resections are combined with a high morbidity and mortality rate [Andersson et al., HPB Surg 2:145-147, 1990; Adamek et al., Scand J Gastroenterol 34:1157-1161, 1999]. Hepatic resection only fit the cases in which the stones localized in one lobe or segment, while it doesn't fit the cases which have polystones in left and right biliary tract. Duodenoscope can only get the stones in the common bile duct and cannot deal with the intrahepatic lithiasis. The management of intrahepatic lithiasis can only be treated by intraoperative or postoperative choledochoscope. For big stones or compact stones, lithotripsy should be applied. But the laser lithotripsy and the electrohydraulic lithotripsy can cause serious complications such as perforation of bile duct. It needs a safer and more reliable treatment for intrahepatic lithiasis. The aim of our work is to study the lithotrity treatment of intrahepatic lithiasis by using helix hydro-jet under Video Choledochoscope. MATERIALS AND METHODS: From March 31, 2003 to October 20, 2004, 30 intrahepatic stone patients were treated. Eighteen of them were women and 12 were men, with ages ranging from 35 to 80 years (mean, 58 years). According to B ultrasound and computed tomography (CT) scan report, there were five cases of intrahepatic lithiasis and common bile duct stones, 25 cases of left and right hepatic duct stones, and one case with giant intrahepatic stone (1.5 x 1.5 x 1.2 cm). Intraoperative or postoperative choledochoscopic helix hydro-jet lithotripsy was applied through a video choledochoscope. For the patients to have the intraoperative choledochoscopic helix hydro-jet lithotripsy, they should be diagnosed correctly by B ultrasound or CT scan. The biliary tract reconstruction by spiral CT scan is as helpful as MRCP or ERCP for clinical diagnosis. For the patients to have the postoperative choledochoscopic helix hydro-jet lithotripsy, they should be diagnosed correctly by T-tube cholangiography and BUS and CT scan. All patients should be verified without stones remaining in the bile duct after lithotripsy by choledochoscopic examination and T-tube cholangiography, and should be examined by BUS again after 6 months to 1 year. We decide whether complications occurred by observation of symptoms and signs after choledochoscopy and lithotripsy. RESULTS: Seventy-five intrahepatic stones with diameter ranging from 0.6 to 1.5 cm were successfully fragmentized in 30 patients using of helix hydro-jet lithotripsy. These fragmentized stones mainly are bilirubin stones. The lithotripsy was carried for 45 times and the procedure needs 1-1.5 h. Helix hydro-jet lithotripsy are used in 16 cases during operation and 12 cases after operation; two cases during operation and after operation. Intrahepatic calculosis was cleaned out completely and verified by postoperative choledochoscope examination and postoperative T-tube cholangiography examination. No complications were observed. CONCLUSION: Helix hydro-jet lithotripsy under video choledochoscope is a safe and effective method for the removal of intrahepatic stone. No bile duct damnified and perforation was observed. The procedure is without pain and heat, and the pressure can be adjusted easily. The research provides a new way of using the helix hydro-jet, and a new way of curing the intrahepatic lithiasis.