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Asian Pac J Cancer Prev ; 19(11): 3065-3069, 2018 Nov 29.
Article in English | MEDLINE | ID: mdl-30485942

ABSTRACT

Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells, selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by flow cytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.Result: We found that tylophorine did not significantly influence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation of cells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptotic proteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin. Conclusions: Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cells through modulation of the cell cycle and affecting the expression of caspases 3 and 9.


Subject(s)
Alkaloids/pharmacology , Antibiotics, Antineoplastic/pharmacology , Apoptosis/drug effects , Breast Neoplasms/pathology , Cell Cycle/drug effects , Doxorubicin/pharmacology , Indolizines/pharmacology , Phenanthrenes/pharmacology , Breast Neoplasms/drug therapy , Cell Proliferation/drug effects , Drug Therapy, Combination , Female , Humans , Tumor Cells, Cultured
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