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1.
Proteins ; 62(4): 1044-52, 2006 Mar 01.
Article in English | MEDLINE | ID: mdl-16385579

ABSTRACT

Crosslinking of small heat-shock proteins (sHsps) by tissue transglutaminase (tTG) is enhanced by stress and under pathological conditions. We here used hexapeptide probes to determine the amine donor (K) and acceptor (Q) sites for tTG in Hsp20. Mass spectrometric peptide mass fingerprinting and peptide fragmentation established that Q31 and the C-terminal K162 are involved in inter- and intramolecular crosslinking (transamidation). Q31 is a conserved glutamine in sHsps where the neighboring residue determines its reactivity. Moreover, we detected highly efficient simultaneous deamidation of Q66, which suggests that tTG-catalyzed transamidation and deamidation is specific for different glutamine residues.


Subject(s)
HSP20 Heat-Shock Proteins/metabolism , Transglutaminases/metabolism , Amides/metabolism , Cloning, Molecular , Escherichia coli , GTP-Binding Proteins , HeLa Cells , Humans , Protein Glutamine gamma Glutamyltransferase 2 , Recombinant Proteins/metabolism , Transfection
2.
Eur J Biochem ; 271(21): 4195-203, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15511225

ABSTRACT

The mammalian small heat shock protein alphaB-crystallin can be phosphorylated at three different sites, Ser19, Ser45 and Ser59. We compared the intracellular distribution of wild-type, nonphosphorylatable and all possible pseudophosphorylation mutants of alphaB-crystallin by immunoblot and immunocytochemical analyses of stable and transiently transfected cells. We observed that pseudophosphorylation at two (especially S19D/S45D) or all three (S19D/S45D/S59D) sites induced the partial translocation of alphaB-crystallin from the detergent-soluble to the detergent-insoluble fraction. Double immunofluorescence studies showed that the pseudophosphorylation mutants localized in nuclear speckles containing the splicing factor SC35. The alphaB-crystallin mutants in these speckles were resistant to mild detergent treatment, and also to DNase I or RNase A digestion, indicating a stable interaction with one or more speckle proteins, not dependent on intact DNA or RNA. We further found that FBX4, an adaptor protein of the ubiquitin-protein isopeptide ligase SKP1/CUL1/F-box known to interact with pseudophosphorylated alphaB-crystallin, was also recruited to SC35 speckles when cotransfected with the pseudophosphorylation mutants. Because SC35 speckles also react with an antibody against alphaB-crystallin endogenously phosphorylated at Ser45, our findings suggest that alphaB-crystallin has a phosphorylation-dependent role in the ubiquitination of a component of SC35 speckles.


Subject(s)
Cell Nucleus/metabolism , F-Box Proteins/chemistry , Nuclear Proteins/metabolism , Ribonucleoproteins/metabolism , alpha-Crystallin B Chain/chemistry , Binding Sites , Deoxyribonuclease I/metabolism , Detergents/pharmacology , Endopeptidases/metabolism , HeLa Cells , Humans , Immunoblotting , Immunohistochemistry , Microscopy, Fluorescence , Mutation , Phosphorylation , Plasmids/metabolism , Protein Binding , Protein Transport , Ribonuclease, Pancreatic/metabolism , Serine/chemistry , Serine-Arginine Splicing Factors , Subcellular Fractions/metabolism , Ubiquitin/metabolism , alpha-Crystallin B Chain/metabolism
3.
FEBS Lett ; 576(1-2): 57-62, 2004 Oct 08.
Article in English | MEDLINE | ID: mdl-15474010

ABSTRACT

Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20 and HspB8 are both lysine- and glutamine-donors, alphaB-crystallin only is a lysine-donor, HspB2 a glutamine-donor, and HspB3 no substrate at all. Close interaction of proteins stimulates crosslinking efficiency as crosslinking between different sHsps only takes place within the same heteromeric complex. We also observed that alphaB-crystallin, Hsp27 and Hsp20 associate with Abeta in vitro, and can be readily crosslinked by tTG.


Subject(s)
Amyloid beta-Peptides/metabolism , Cross-Linking Reagents/metabolism , Heat-Shock Proteins/metabolism , Transglutaminases/metabolism , Blotting, Western , Catalysis , Electrophoresis, Polyacrylamide Gel , Heat-Shock Proteins/genetics , Humans , Recombinant Proteins/metabolism
4.
Proteins ; 57(1): 109-17, 2004 Oct 01.
Article in English | MEDLINE | ID: mdl-15326597

ABSTRACT

Small heat shock proteins (sHSPs), which range in monomer size between 12 and 42 kDa, are characterized by a conserved C-terminal alpha-crystallin domain of 80-100 residues. They generally form large homo- or heteromeric complexes, and typically have in vitro chaperone-like activity, keeping unfolding proteins in solution. A special type of sHSP, with a duplicated alpha-crystallin domain, is present in parasitic flatworms (Platyhelminthes). Considering that an alpha-crystallin domain is essential for the oligomerization and chaperone-like properties of sHSPs, we characterized Tsp36 from the tapeworm Taenia saginata. Both wild-type Tsp36 and a mutant (Tsp36C-->R) in which the single cysteine has been replaced by arginine were expressed and purified. Far-UV CD measurements of Tsp36 were in agreement with secondary structure predictions, which indicated alpha-helical structure in the N-terminal region and the expected beta-sandwich structure for the two alpha-crystallin domains. Gel permeation chromatography and nano-ESI-MS showed that wild type Tsp36 forms dimers in a reducing environment, and tetramers in a non-reducing environment. The tetramers are stabilized by disulfide bridges involving a large proportion of the Tsp36 monomers. Tsp36C-->R exclusively occurs as dimers according to gel permeation chromatography, while the nondisulfide bonded fraction of wild type Tsp36 dissociates from tetramers into dimers under nonreducing conditions at increased temperature (43 degrees C). The tetrameric form of Tsp36 has a greater chaperone-like activity than the dimeric form.


Subject(s)
Heat-Shock Proteins/chemistry , Helminth Proteins/chemistry , Molecular Chaperones/chemistry , Taenia saginata/chemistry , Amino Acid Sequence , Amino Acid Substitution , Circular Dichroism , Citrate (si)-Synthase/chemistry , Cysteine/chemistry , Disulfides/chemistry , Insulin/chemistry , Multiprotein Complexes/chemistry , Mutagenesis, Site-Directed , Oxidation-Reduction , Protein Structure, Secondary , Protein Structure, Tertiary , Recombinant Proteins/chemistry
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