ABSTRACT
The accurate and comprehensive identification of functional regulatory sequences in mammalian genomes remains a major challenge. Here we describe site-specific integration fluorescence-activated cell sorting followed by sequencing (SIF-seq), an unbiased, medium-throughput functional assay for the discovery of distant-acting enhancers. Targeted single-copy genomic integration into pluripotent cells, reporter assays and flow cytometry are coupled with high-throughput DNA sequencing to enable parallel screening of large numbers of DNA sequences. By functionally interrogating >500 kilobases (kb) of mouse and human sequence in mouse embryonic stem cells for enhancer activity we identified enhancers at pluripotency loci including NANOG. In in vitro-differentiated cardiomyocytes and neural progenitor cells, we identified cardiac enhancers and neuronal enhancers, respectively. SIF-seq is a powerful and flexible method for de novo functional identification of mammalian enhancers in a potentially wide variety of cell types.
Subject(s)
Embryonic Stem Cells/cytology , Enhancer Elements, Genetic , Myocytes, Cardiac/cytology , Neural Stem Cells/cytology , Animals , Cell Differentiation , Cell Separation , Chromosomes, Artificial, Bacterial/genetics , Flow Cytometry , Gene Expression Regulation , Gene Library , Genes, Reporter , Genetic Vectors , Genomics , High-Throughput Nucleotide Sequencing , Humans , Mice , Mice, Transgenic , Plasmids/metabolism , Sequence Analysis, DNAABSTRACT
The Iroquois homeobox (Irx) homeodomain transcription factors are important for several aspects of embryonic development. In the developing heart, individual Irx genes are important for certain postnatal cardiac functions, including cardiac repolarization (Irx5) and rapid ventricular conduction (Irx3). Irx genes are expressed in dynamic and partially overlapping patterns in the developing heart. Here we show in mice that Irx3 and Irx5 have redundant function in the endocardium to regulate atrioventricular canal morphogenesis and outflow tract formation. Our data suggest that direct transcriptional repression of Bmp10 by Irx3 and Irx5 in the endocardium is required for ventricular septation. A postnatal deletion of Irx3 and Irx5 in the myocardium leads to prolongation of atrioventricular conduction, due in part to activation of expression of the Na(+) channel protein Nav1.5. Surprisingly, combined postnatal loss of Irx3 and Irx5 results in a restoration of the repolarization gradient that is altered in Irx5 mutant hearts, suggesting that postnatal Irx3 activity can be repressed by Irx5. Our results have uncovered complex genetic interactions between Irx3 and Irx5 in embryonic cardiac development and postnatal physiology.
Subject(s)
Heart/embryology , Heart/physiology , Homeodomain Proteins/metabolism , Transcription Factors/metabolism , Animals , Chromatin Immunoprecipitation , Electrophysiology , Female , Heart Ventricles/embryology , Heart Ventricles/metabolism , Homeodomain Proteins/genetics , Immunoprecipitation , Mice , Pregnancy , Transcription Factors/geneticsABSTRACT
Heart development is exquisitely sensitive to the precise temporal regulation of thousands of genes that govern developmental decisions during differentiation. However, we currently lack a detailed understanding of how chromatin and gene expression patterns are coordinated during developmental transitions in the cardiac lineage. Here, we interrogated the transcriptome and several histone modifications across the genome during defined stages of cardiac differentiation. We find distinct chromatin patterns that are coordinated with stage-specific expression of functionally related genes, including many human disease-associated genes. Moreover, we discover a novel preactivation chromatin pattern at the promoters of genes associated with heart development and cardiac function. We further identify stage-specific distal enhancer elements and find enriched DNA binding motifs within these regions that predict sets of transcription factors that orchestrate cardiac differentiation. Together, these findings form a basis for understanding developmentally regulated chromatin transitions during lineage commitment and the molecular etiology of congenital heart disease.
Subject(s)
Epigenesis, Genetic , Gene Regulatory Networks , Myocardium/cytology , Animals , Cell Differentiation , Chromatin/metabolism , Embryonic Stem Cells/metabolism , Enhancer Elements, Genetic , Heart/embryology , Humans , Mice , Transcription Factors/metabolism , TranscriptomeABSTRACT
Rapid electrical conduction in the His-Purkinje system tightly controls spatiotemporal activation of the ventricles. Although recent work has shed much light on the regulation of early specification and morphogenesis of the His-Purkinje system, less is known about how transcriptional regulation establishes impulse conduction properties of the constituent cells. Here we show that Iroquois homeobox gene 3 (Irx3) is critical for efficient conduction in this specialized tissue by antithetically regulating two gap junction-forming connexins (Cxs). Loss of Irx3 resulted in disruption of the rapid coordinated spread of ventricular excitation, reduced levels of Cx40, and ectopic Cx43 expression in the proximal bundle branches. Irx3 directly represses Cx43 transcription and indirectly activates Cx40 transcription. Our results reveal a critical role for Irx3 in the precise regulation of intercellular gap junction coupling and impulse propagation in the heart.
Subject(s)
Bundle of His/physiology , Heart Conduction System , Homeodomain Proteins/physiology , Purkinje Fibers/physiology , Transcription Factors/physiology , Animals , Connexin 43/genetics , Connexins/genetics , Gap Junctions , Gene Expression Regulation , Genes, Homeobox , Heart Ventricles , Mice , Transcription, GeneticABSTRACT
Dominant mutations in cardiac transcription factor genes cause human inherited congenital heart defects (CHDs); however, their molecular basis is not understood. Interactions between transcription factors and the Brg1/Brm-associated factor (BAF) chromatin remodelling complex suggest potential mechanisms; however, the role of BAF complexes in cardiogenesis is not known. In this study, we show that dosage of Brg1 is critical for mouse and zebrafish cardiogenesis. Disrupting the balance between Brg1 and disease-causing cardiac transcription factors, including Tbx5, Tbx20 and Nkx2-5, causes severe cardiac anomalies, revealing an essential allelic balance between Brg1 and these cardiac transcription factor genes. This suggests that the relative levels of transcription factors and BAF complexes are important for heart development, which is supported by reduced occupancy of Brg1 at cardiac gene promoters in Tbx5 haploinsufficient hearts. Our results reveal complex dosage-sensitive interdependence between transcription factors and BAF complexes, providing a potential mechanism underlying transcription factor haploinsufficiency, with implications for multigenic inheritance of CHDs.
Subject(s)
DNA Helicases/metabolism , Heart Defects, Congenital/genetics , Heart/embryology , Morphogenesis/physiology , Multiprotein Complexes/metabolism , Nuclear Proteins/metabolism , Transcription Factors/metabolism , Zebrafish Proteins/metabolism , Adaptor Proteins, Signal Transducing , Animals , Chromatin Immunoprecipitation , DNA Helicases/genetics , DNA Primers/genetics , Echocardiography , Electrocardiography , Gene Dosage , Haploinsufficiency , Homeobox Protein Nkx-2.5 , Homeodomain Proteins/metabolism , Mice , Microarray Analysis , Morphogenesis/genetics , NIH 3T3 Cells , Nuclear Proteins/genetics , T-Box Domain Proteins/metabolism , Transcription Factors/genetics , Zebrafish , Zebrafish Proteins/geneticsABSTRACT
Dysmorphogenesis of the cardiac outflow tract (OFT) causes many congenital heart defects, including those associated with DiGeorge syndrome. Genetic manipulation in the mouse and mutational analysis in patients have shown that Tbx1, a T-box transcription factor, has a key role in the pathogenesis of this syndrome. Here, we have dissected Tbx1 function during OFT development using genetically modified mice and tissue-specific deletion, and have defined a dual role for this protein in OFT morphogenesis. We show that Tbx1 regulates cell contribution to the OFT by supporting cell proliferation in the secondary heart field, a source of cells fated to the OFT. This process might be regulated in part by Fgf10, which we show for the first time to be a direct target of Tbx1 in vitro. We also show that Tbx1 expression is required in cells expressing Nkx2.5 for the formation of the aorto-pulmonary septum, which divides the aorta from the main pulmonary artery. These results explain why aortic arch patterning defects and OFT defects can occur independently in individuals with DiGeorge syndrome. Furthermore, our data link, for the first time, the function of the secondary heart field to congenital heart disease.
Subject(s)
Gene Expression Regulation, Developmental , Heart/embryology , Myocardium/metabolism , T-Box Domain Proteins/physiology , Alleles , Animals , Bromodeoxyuridine/pharmacology , Cell Differentiation , Cell Division , Coloring Agents/pharmacology , DNA Mutational Analysis , DiGeorge Syndrome/genetics , Endothelial Cells/metabolism , Fibroblast Growth Factor 10 , Fibroblast Growth Factors/metabolism , Gene Deletion , Homeobox Protein Nkx-2.5 , Homeodomain Proteins/metabolism , Immunohistochemistry , In Situ Hybridization , Luciferases/metabolism , Mesoderm/metabolism , Mice , Mice, Inbred C57BL , Mice, Transgenic , Models, Biological , Models, Genetic , Mutation , Myocytes, Cardiac/metabolism , Reverse Transcriptase Polymerase Chain Reaction , T-Box Domain Proteins/metabolism , Transcription Factors/metabolismABSTRACT
Transcriptional cascades responsible for initiating the formation of vertebrate embryonic structures such as limbs are not well established. Limb formation occurs as a result of interplay between fibroblast growth factor (FGF) and Wnt signaling. What initiates these signaling cascades and thus limb bud outgrowth at defined locations along the anteroposterior axis of the embryo is not known. The T-box transcription factor TBX5 is important for normal heart and limb formation, but its role in early limb development is not well defined. We report that mouse embryos lacking Tbx5 do not form forelimb buds, although the patterning of the lateral plate mesoderm into the limb field is intact. Tbx5 is not essential for an early establishment of forelimb versus hindlimb identity. In the absence of Tbx5, the FGF and Wnt regulatory loops required for limb bud outgrowth are not established, including initiation of Fgf10 expression. Tbx5 directly activates the Fgf10 gene via a conserved binding site, providing a simple and direct mechanism for limb bud initiation. Lef1/Tcf1-dependent Wnt signaling is not essential for initiation of Tbx5 or Fgf10 transcription, but is required in concert with Tbx5 for maintenance of normal levels of Fgf10 expression. We conclude that Tbx5 is not essential for the early establishment of the limb field in the lateral plate mesoderm but is a primary and direct initiator of forelimb bud formation. These data suggest common pathways for the differentiation and growth of embryonic structures downstream of T-box genes.