ABSTRACT
INTRODUCTION: TNF-α, and its soluble form sTNFR1, as proinflammatory hormone plays a great role in pathogenesis of auto immunological diseases, insulin resistance, both carbohydrates and fat metabolism and development of late complications in type 1 diabetes mellitus (DMT1) patients. AIM OF THE STUDY: To asses TNF-α and sTNFR1 levels in children with DMT1 and to analyze the correlation with anthropometric parameters, metabolic control and the influence of the kind of insulin therapy. MATERIAL AND METHODS: 67 patients, aged from 3.71 to 14.81 years (mean±SD: 10.33±2.21). All the children were prepubertal (T â°2), suffering for DMT1, without any coexisting diseases. The duration of the disease varied from 1.83 to 9 years (mean±SD: 4,0±1,6), and the age at diagnosis oscillated between 1.84 to 10.81 years. All the patients were divided into subgroups according to the kind of therapy which was not changed in the last 6 months. 15 agematched healthy children were included into the study. There were no statistically significant differences between the groups as to the metabolic control, age, weight, height and BMI. RESULTS: TNF-α levels in the study group ranged from 0.30 to 14.20 ng/ml (mean±1.62 ± 0.41 pg/ml) and were lower than in the control group: from 0.20 to 19.00 pg/ml (mean±SD: 1.67±1.41 pg/ml) (p >0.05). The highest TNF-α levels were observed in the insulin pump group, lower in the multiple insulin injection group and in the conventional insulin therapy group. The sTNFR1 in the study group ranged from 707.00 to 1646.00 pg/ml (mean±SD: 1028.18±181.24 pg/ml) and was higher than in the control group: 613.0 to 1310.00 pg/ml (mean±SD: 978.00±203.03 pg/ml) (p >0.05). The highest sTNFR1 levels were observed in the insulin pump group, lower in the multiple insulin injection group and the lowest in the conventional insulin therapy group. CONCLUSIONS: TNF-α levels are lower in DMT1 children, correlate only with height and do not depend on the kind of insulin therapy. sTNFR1 levels are higher in children with DMT1, correlate with anthropometric parameters and do not depend on the kind of insulin therapy.
Subject(s)
Diabetes Mellitus, Type 1/blood , Receptors, Tumor Necrosis Factor, Type I/blood , Tumor Necrosis Factor-alpha/metabolism , Adolescent , Body Mass Index , Case-Control Studies , Child , Child Welfare , Child, Preschool , Diabetes Mellitus, Type 1/drug therapy , Female , Humans , Insulin/therapeutic use , Male , Poland , Reference ValuesABSTRACT
INTRODUCTION: Leptin and soluble form of leptin receptor play a great role in both carbohydrate and fat metabolism which is very important in diabetes mellitus type (DMT1) patients. AIM OF THE STUDY: To asses leptin and soluble leptin receptor levels in children with DMT1 and to analyze correlation with anthropometric parameters, metabolic control and the influence of various kinds of insulin therapy. MATERIAL AND METHODS: 67 patients, aged from 3.71 to 14.81 years, mean ± SD: 10.33 ± 2.21) and 15 age-matched healthy children were included into the study. All children were prepubertal (T <2), suffering for DMT1 for more than two years, without any coexisting diseases. All patients were divided into subgroups according to the kind of therapy. There were no statistically significant differentials between groups as to the metabolic control, age, weight, height and BMI. RESULTS: Leptin levels in the study group range from 1.27 to 59.90 ng/ml (mean ± SD: 11.34 ± 9.42 ng/ml) and were higher than in control group: from 1.46 to 26, ng/ml (mean ± SD: 8.23 ± 7.62 ng/ml) (p >0.05). The highest leptin levels were observed in multiple insulin injection group, lower in pump group and the lowest conventional insulin therapy group. Soluble leptin receptor levels in study group range from 30.50 to 101.00 ng/ml (mean ± SD: 48.65 ± 13.26 ng/ ml) and were significantly higher than in control group: 27.50 to 61.00 ng/ml (mean ± SD: 39.37 ± 9.23 ng/ml) (p <0.01). The highest soluble leptin receptor levels were observed in the multiple insulin injection group, lower in pump group and the lowest conventional insulin therapy group. CONCLUSIONS: leptin levels are higher in DMT1 children, correlate with anthropometrics parameters, age, age at the beginning of diabetes and depend on the kind of insulin therapy. Soluble leptin receptor levels are significantly higher in children with DMT1, correlate with anthropometrics parameters, metabolic control, age, age at the beginning of diabetes, duration of diabetes and depend on the kind of insulin therapy.
