ABSTRACT
A germplasm line Shannong05078 was developed from the progenies of Am3 x Triticum aestivum-Dasypyrum villosum amphidiploid, the purpose of this study is to ascertain the genome composition of Shannong05078 and then supply wheat breeding with reference. The results showed that Shannong05078 was stable in morphology characters and presenting favorable agronomic traits, it was resistant to powdery mildew and rust. Its protein content was higher, and the HWM-GS of it was 7+8, 5+10. The chromosome number of Shannong05078 was 2n=28, and the chromosome configuration was 2n=14 II at the PMC MI. SSR analysis showed that Shannong05078 contained the whole A and B genomes, and had little genetic materials from D genome. A specific primer of Dasypyrum villosum (phv29) was used to identify the genetic materials form Dasypyrum villosum in Shannong05078, and specific band of Dasypyrum villosum appeared in it. It can be deduced that the genomic composition of Shannong05078 is 2n=AABB=28, and genetic materials of D genome and Dasypyrum villosum were translated into Shannong05078.
Subject(s)
Chimera/genetics , Crosses, Genetic , Poaceae/genetics , Triticum/genetics , Chimera/metabolism , Chromosomes, Plant/genetics , Genome, Plant , Hybridization, Genetic , Plant Proteins/genetics , Plant Proteins/metabolism , Poaceae/metabolism , Triticum/metabolismABSTRACT
Objective: To study the influence of negative electrets on apoptosis of fibroblast cells and to probe its mechanism. Methods: Fibroblast cell were treated with -300, -500 and -1 000V PTFE electrets for 24, 48 and 72 h, respectively, and the influence of negative electrets on cell apoptosis was studied by means of flow cytometry and transmission electron microscope. Results: Compared with control group, apoptosis cells increased from 0.5% to 10% (some even to 15%) after 24，48 and 72 h action of -300, -500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles (apoptotic bodies). The effect of negative electrets on apoptosis was in proportion to the time and electric field intensity. Conclusion: Negative electrets can enhance apoptosis of fibroblast cells.
ABSTRACT
Objective: To study the influence of negative electrets on apoptosis of fibroblast cells and to probe its mechanism. Methods: Fibroblast cell were treated with -300, -500 and -1 000V PTFE electrets for 24, 48 and 72 h, respectively, and the influence of negative electrets on cell apoptosis was studied by means of flow cytometry and transmission electron microscope. Results: Compared with control group, apoptosis cells increased from 0.5% to 10% (some even to 15%) after 24，48 and 72 h action of -300, -500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles (apoptotic bodies). The effect of negative electrets on apoptosis was in proportion to the time and electric field intensity. Conclusion: Negative electrets can enhance apoptosis of fibroblast cells.
