ABSTRACT
A simple enzymatic colorimetric procedure for the total serum bile acids determination was evaluated. In 518 normal patients values of statistical analysis ranged from 0.60 to 7.75 mumol/l (mean = 3.38 and S.D. = 2.00).
Subject(s)
Bile Acids and Salts/blood , Clinical Enzyme Tests , Colorimetry , Evaluation Studies as Topic , Humans , Reagent Kits, Diagnostic , Reference ValuesABSTRACT
1077 serum samples collected from patients of both sexes showing values of creatininemia less than or equal to 1.4 mg/dl as evaluated by the enzymatic colorimetric method (Wako reagents, Cobas Fara analyzer) have been processed using 430 Selective Analyzer Sclavo. Test for imprecision and inaccuracy within-run and between-day (40) showing very low values (less than 3%) of variation and bias coefficients, preliminarily demonstrated the reliability of the automatic fixed time Jaffé's reaction. Statistical analysis gives these values: means = 0.66 mg/dl, deviation standard (DS) = 0.20 mg/dl, means + 2 DS = 1.06 mg/dl, in good agreement with data provided by the enzymatic procedure.