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1.
Clin Immunol ; 145(3): 177-88, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23103487

ABSTRACT

Evidences indicate that pregnancy can alter the Ag-specific T-cell responses. This work aims to evaluate the impact of pregnancy on the in vitro HIV-1-specific immune response. As compared with non-pregnant patients, lower T-cell proliferation and higher IL-10 production were observed in T-cell cultures from pregnant patients following addition of either mitogens or HIV-1 antigens. In our system, the main T lymphocyte subset involved in producing IL-10 was CD4(+)FoxP3(-). Depletion of CD4(+) cells elevated TNF-α and IFN-γ production. Interestingly, the in vitro HIV-1 replication was lower in cell cultures from pregnant patients, and it was inversely related to IL-10 production. In these cultures, the neutralization of IL-10 by anti-IL-10 mAb elevated TNF-α release and HIV-1 replication. In conclusion, our results reveal that pregnancy-related events should favor the expansion of HIV-1-specific IL-10-secreting CD4(+) T-cells in HIV-1-infected women, which should, in the scenario of pregnancy, help to reduce the risk of vertical HIV-1 transmission.


Subject(s)
HIV Infections/complications , HIV Infections/immunology , HIV-1/immunology , Pregnancy Complications, Infectious/immunology , T-Lymphocyte Subsets/immunology , Adult , Case-Control Studies , Female , HIV Antigens/administration & dosage , HIV Infections/transmission , HIV Infections/virology , HIV-1/physiology , Humans , In Vitro Techniques , Infant, Newborn , Infectious Disease Transmission, Vertical/prevention & control , Interleukin-10/biosynthesis , Lymphocyte Activation , Pregnancy , Pregnancy Complications, Infectious/virology , T-Lymphocyte Subsets/virology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/virology , Virus Replication/immunology , Young Adult
2.
Clin Immunol ; 145(1): 31-43, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22922271

ABSTRACT

This work aims to elucidate the effects of age and HIV-1 infection on the frequency and function of T cell subsets in response to HIV-specific and non-specific stimuli. As compared with the younger AIDS group, the frequencies of naive and central memory T cells were significantly lower in aged AIDS patients. Although there was also a dramatic loss of classical CD4(+)FoxP3(+)CD25(+)Treg cells in this patient group, high frequencies of IL-10-producing CD4(+)FoxP3(-) T cells were observed. In our system, the increased production of IL-10 in aged AIDS patients was mainly derived from Env-specific CD4(+)FoxP3(-)CD152(+) T cells. Interestingly, while the blockade of IL-10 activity by monoclonal antibody clearly enhanced the release of IL-6 and IL-1ß by Env-stimulated PBMC cultures from aged AIDS patients, this monoclonal antibody enhanced in vitro HIV-1-replication. In conclusion, HIV infection and aging undoubtedly contribute synergistically to a complex immune dysfunction in T cell compartment of HAART-treated older HIV-infected individuals.


Subject(s)
Acquired Immunodeficiency Syndrome/immunology , HIV-1/drug effects , Interleukin-10/immunology , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Regulatory/immunology , Acquired Immunodeficiency Syndrome/drug therapy , Acquired Immunodeficiency Syndrome/virology , Adult , Age Factors , Antibodies, Monoclonal/pharmacology , Antiretroviral Therapy, Highly Active , Antiviral Agents/administration & dosage , Cells, Cultured , Female , HIV-1/physiology , Humans , Immunologic Memory , Immunophenotyping , Interleukin-10/antagonists & inhibitors , Interleukin-1beta/biosynthesis , Interleukin-1beta/immunology , Interleukin-6/biosynthesis , Interleukin-6/immunology , Male , Middle Aged , T-Lymphocyte Subsets/drug effects , T-Lymphocyte Subsets/pathology , T-Lymphocytes, Regulatory/drug effects , T-Lymphocytes, Regulatory/pathology , Virus Replication/drug effects
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