ABSTRACT
N-trans-feruloyloctopamine (FO) isolated from Garlic skin was identified as the primary antioxidant constituents, however, the effect of which on HCC invasion is still unclear. Herein, the FO was synthesized and its antitumor activities were evaluated in HCC cell lines. Cellular functional analyses have revealed that the reformed FO owns strong abilities of inhibiting cell proliferation and invasion in HCC cells. Molecular data have further showed that FO could significantly decrease the phosphorylation levels of Akt and p38 MAPK. In addition, the expression of Slug was inhibited and the level of E-cadherin increased. Molecular docking analysis indicates that the H-bond and hydrophobic interactions were critical for FO and E-cadherin binding, but FO did not seem to act directly on phosphorylated Akt and p38 MAPK. We have thus concluded that reformed FO inhibits cell invasion might be directly through EMT related signals (E-cadherin) and indirectly through PI3K/Akt, p38 MAPK signaling pathways. FO might be a promising drug in HCC treatment and prognosis.
Subject(s)
Carcinoma, Hepatocellular/pathology , Coumaric Acids/pharmacology , Epithelial-Mesenchymal Transition/drug effects , Liver Neoplasms/pathology , Neoplasm Invasiveness/prevention & control , Octopamine/analogs & derivatives , Proto-Oncogene Proteins c-akt/metabolism , Signal Transduction/drug effects , p38 Mitogen-Activated Protein Kinases/metabolism , Carcinoma, Hepatocellular/enzymology , Carcinoma, Hepatocellular/metabolism , Cell Line, Tumor , Humans , Liver Neoplasms/enzymology , Liver Neoplasms/metabolism , Octopamine/pharmacologyABSTRACT
The magnetic properties of the partially filled skutterudite Eu(0.5)Co(4)Sb(12) are investigated by a model Hamiltonian, with special emphasis on the effect of ordering and disordering occupancy of the filler atoms Eu on the magnetic properties. The magnetization, magnetic specific heat and entropy are calculated within the mean-field approximation. By introducing the position disorder of the filler atoms, the critical temperature T(C) above which the magnetization disappears is changed. The magnetization curve near T(C) also becomes concave compared to the convex magnetization of the ordered system. The filler disordering also leads to a kink in the isothermal magnetization curve and a valley in the magnetic specific heat near T(C). The effective magnetic field acting on the localized spin of the filler atoms has a competitive effect with the disorder and therefore makes the valley disappear.
