Geochemical Characteristics and Geological Significance of Coalbed Methane in Suzhou Mining Area of the Huaibei Coalfield.

In this study, gas contents, geochemical features, and origins of coalbed methane (CBM) and their influence factors were investigated on nos. 7 and 8 CBM reservoirs from the Suzhou mining area of the Huaibei coalfield. Results have shown that the selected CBM reservoirs are characterized by various thickness (0.50-9.19 m) and buried depth (619-1226 m), but have relatively better lithology of surrounding rocks. Coal samples have similar maturity (Ro,max = 0.71-1.05%), but show differences in chemical composition and macerals. Gas content of nos. 7 and 8 CBM reservoirs ranges from 6.13-12.25 m3/t, but the value of former is lower than that of later one overall. In addition, CH4 is a predominantly component with a value of 88.23-99.00% (avg. 96.69%), and the heavy hydrocarbon gas (C2+) is 0.00-1.93% (avg. 0.41%). The δ13CCH4 value ranges from -64.54 to -46.36‰ (avg. -53.92‰), and the δ13DCH4 value is -224.36 to -211.75‰ (avg. -219.09‰). Based on the analysis of components and isotopic values, the CBM samples are thermogenic (20.92-71.30%; avg 50.09%) and secondary biogenic gases (28.70-74.49%; avg. 49.91%). Gas content shows changeable characteristics at a buried depth of 300-1300 m, which is affected by buried depth, reservoir temperature and pressure, Mad and vitrinite. However, the CH4 concentration shows no correlation with buried depth. Moreover, the buried depth is significantly positively correlated to δ13CCH4 and δ13DCH4. Based on the relationship between gas content and isotope values, it suggests that δ13CCH4 or δ13DCH4 may have a relationship with the main controlling factors of gas content.

[Effects of bosutinib on the malignant behavior of thyroid papillary carcinoma B-CPAP cells and its mechanisms].

Objective: To investigate the effects of bosutinib on the malignant behavior of thyroid papillary carcinoma B-CPAP cells and its possible mechanisms. Methods: Thyroid papillary carcinoma B-CPAP cells were cultured in vitro with a concentration gradient of(1、2、3、4 and 5 µmol/L)bosutinib intervened for 24 hours, DMSO was used as the control group. Five parallel compound holes were set in each group. Cell counting kit (CCK-8 method) method was used to detect cell proliferation. Transwell assay and cell wound healing assay were used to detect cell invasion and migration. TUNEL staining assay and flow cytometry were used to detect cell apoptosis. Western blot was used to detect the expressions of autophagic proteins (Beclin-1, LC3, p62) and signal pathway proteins (SIK2, p-mTOR, mTOR, p-ULK1, ULK1). Results: Compared with the control group, the cell proliferation activity, migration ability and invasion ability were decreased (P＜0.01), while the cell apoptosis rate was increased (P＜0.01) in the bosutinib concentration groups of 2, 3, 4 and 5 µmol/L . In the concentration groups of 4 and 5 µmol/L, the expression of Beclin-1 (P＜0.05), LC3- Ⅱ/LC3- Ⅰ (P＜0.05), SIK2 (P＜0.01) and p-ULK1 (P＜0.01) protein was decreased, while the expression of p62 (P＜ 0.05) and p-mTOR (P＜0.01) protein was increased. Conclusion: Bosutinib may inhibit the autophagy of thyroid papillary carcinoma cells through SIK2-mTOR-ULK1 signaling pathway to inhibit their proliferation, invasion and migration and promote apoptosis, thereby weakening their malignant behavior.

Regulation of TBBPA-induced oxidative stress on mitochondrial apoptosis in L02 cells through the Nrf2 signaling pathway.

Tetrabromobisphenol A (TBBPA) is a commonly used brominated flame retardant, which has a wide range of toxic effects on organisms. This study investigated the cytotoxic effects on human hepatocytes (L02 cells) after treated with 0, 5, 10, 20, and 40 µM of TBBPA. Results showed that TBBPA significantly increased intracellular reactive oxygen species (ROS), malondialdehyde (MDA) and the ratio of oxidized/reduced glutathione (GSSG/GSH) dose-dependently. TBBPA also decreased the cell mitochondrial membrane potential (MMP), caused the release of cytochrome C (Cyt C) to cytoplasm and promoted the expression of caspase-9 and caspase-3, and finally increased the level of apoptosis. The ROS inhibitor N-acetyl-L-cysteine (NAC) relieved the oxidative stress responses, and prevented the decrease of MMP and increase of apoptosis. In addition, TBBPA promoted the expression of antioxidant genes related to Nrf2, such as quinone oxidoreductase 1 (NQO1), catalase (CAT), and heme oxygenase 1 (HO-1). Oxidative stress initiated by TBBPA, activated mitochondrial apoptosis and Nrf2 pathway, and increased the degree of apoptosis in L02 cells.