ABSTRACT
The complete mitochondrial genome of Brachionus rubens was sequenced using primers design, clone culture, DNA extraction, LONG-PCR amplification, purification and clone sequencing. We found that it is composed of two circular chromosomes, designated mtDNA I (11,398 bp) and mtDNA II (12,820 bp). The gene content of the B. rubens mitochondrial genome was similar to that of the previously reported mitochondrial genome of B. plicatilis. It contained 22 tRNA genes, 2 rRNA genes and 12 protein-coding genes (PCGs). Four of the 12 PCGs had an incomplete stop codons, TAï¼cob, atp6, nd3ï¼or T(cox3). The A + T content of B. rubens mitochondrial genome was apparently higher (mtDNA-I 70.2% and mtDNA II 70.4%) than that of the mitochondrial genome of B. plicatilis (mtDNA-I 63.9% and mtDNA-II 62.9%).
ABSTRACT
The mitochondrial genome of Zacco platypus (Cypriniformes: Cyprinidae, subfamily Daninninae) is a circular molecule of 16,611 bp in length, containing 37 typical animal mitochondrial genes: 13 protein-coding genes (PCGs), 2 ribosomal RNAs, 22 transfer RNAs and a D-loop region. Its gene order and arrangement are identical to the common type found in most fish mitogenomes. All PCGs start with a typical ATG codon except for COI which use GTG as start codon; all PCGs terminate in the common stop codon TAA or TAG, except for the COII which use single T as stop codon. The D-loop region is 928 bp long, located between tRNAPro and tRNAPhe genes. It contains some structures of repeated motifs and microsatellite-like elements characteristic of the Cyprinidae.
Subject(s)
Cyprinidae/genetics , Genome, Mitochondrial , Sequence Analysis, DNA/methods , Animals , Base Composition , Gene Order , Genome Size , Molecular Sequence DataABSTRACT
The mitochondrial genome of Acrossocheilus fasciatus (Cyprinidae, Barbinae) is a circular molecule of 16,589 bp in length, containing 37 typical animal mitochondrial genes: 13 protein-coding genes (PCGs), 2 r RNAs, 22 t RNAs and a non-coding D-loop region. Its gene order and arrangement are identical to the common type found in most fish mitogenomes. All PCGs start with a typical ATG codon except for COI which use GTG as a start codon; all PCGs terminate in the common stop codon TAA or TAG, except for the ND2, ND3, ND4, COII, Cytb and COIII which use single T or TA as a stop codon. The non-coding D-loop region is 938 bp long, located between tRNAPro and tRNAPhe genes. It contains some structures of repeated motifs and microsatellite-like elements characteristic of the Cyprinidae.