ABSTRACT
BACKGROUND: Circular RNAs (circRNAs) have critical roles in various types of diseases, including preeclampsia (PE). Circ_0005714 function in PE was explored in this study. METHODS: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed for level analysis of circ_0005714, micoRNA-223-3p (miR-223-3p), and a disintegrin and metalloproteinase 9 (ADAM9). Cell Counting Kit-8 (CCK-8) and colony formation assays were used for cell viability and colony formation detection. Cell proliferation was determined by EdU assay. The determination of migration and invasion was conducted by wound healing assay and transwell assay. Tube formation assay was applied to assess angiopoiesis. Target binding analysis was performed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Western blot was used for protein examination. RESULTS: Circ_0005714 was highly expressed in PE placenta tissues. The expression promotion of circ_0005714 reduced proliferation, migration, invasion, and angiopoiesis in trophoblast cells. Furthermore, circ_0005714 acted as a molecular sponge for miR-223-3p and the effects of circ_0005714 on trophoblast cells were achieved by sponging miR-223-3p. Moreover, miR-223-3p could target ADAM9 and knockdown of ADAM9 reversed cell progression inhibition induced by miR-223-3p inhibitor. In addition, circ_0005714 upregulated the ADAM9 expression and inactivated the Wnt/ß-catenin pathway through targeting miR-223-3p. CONCLUSIONS: All results manifested that circ_0005714 retarded the progression of PE by mediating the miR-223-3p/ADAM9 signal network.
Subject(s)
ADAM Proteins , MicroRNAs , RNA, Circular , ADAM Proteins/genetics , ADAM Proteins/metabolism , Cell Line, Tumor , Cell Movement/genetics , Cell Proliferation/genetics , Disintegrins/genetics , Disintegrins/metabolism , Female , Humans , Membrane Proteins/genetics , MicroRNAs/genetics , MicroRNAs/metabolism , Pregnancy , RNA, Circular/genetics , Trophoblasts/metabolism , beta Catenin/metabolismABSTRACT
PURPOSE: We aimed to evaluate the efficacy and safety of helical tomotherapy (HT) combined with computed tomography (CT)-guided three-dimensional intracavitary brachytherapy (CT-ICBT) in the treatment of locally advanced cervical cancer. METHODS: A total of 96 patients with locally advanced cervical cancer (IIB-IIIB) treated were retrospectively analyzed. They underwent concurrent radiochemotherapy, and the chemotherapy regimen paclitaxel + cisplatin was given for 3 weeks. The patients were divided into HT+CT-ICBT group (n=48) and intensity-modulated radiotherapy (IMRT) + two-dimensional ICBT (IMRT+ICBT group, n=48) according to the different extracorporeal and intracavitary radiotherapies. The short-term clinical efficacy, and short- and long-term adverse reactions were compared between the two groups, the tumor recurrence and survival status were recorded through follow-up, and the overall survival (OS) and progression-free survival (PFS) rates were compared between the two groups. RESULTS: The patient general clinical characteristics were comparable in both groups. The short-term clinical effective rate was 91.7% (44/48) and 87.5% (42/48), respectively, in HT+CT-ICBT group and IMRT+ICBT group. In the two groups, the incidence rate of grade 3-4 chronic radiation proctitis was 4.2% (2/48) and 22.9% (11/48), while that of grade 3-4 chronic radiation cystitis was 2.1% (1/48) and 18.7% (9/48), respectively. According to the follow-up results, the 3-year OS was 85.4% (41/48) and 77.1% (37/48), and the 3-year PFS was 81.3% (39/48) and 70.8% (34/48), respectively, in the two groups. Log-rank test showed that the 3-year OS and PFS had no statistically significant differences (p=0.395, p=0.401). CONCLUSION: HT+CT-ICBT is safe and effective in the treatment of locally advanced cervical cancer, and it has similar short-term clinical efficacy and long-term survival rate compared with IMRT+ICBT, which also significantly reduces the long-term incidence of radiation proctitis and cystitis, so it is worthy of popularization and application.
Subject(s)
Brachytherapy/methods , Imaging, Three-Dimensional , Radiotherapy, Image-Guided , Radiotherapy, Intensity-Modulated , Tomography, X-Ray Computed , Uterine Cervical Neoplasms/radiotherapy , Adult , Combined Modality Therapy , Female , Humans , Middle Aged , Neoplasm Staging , Retrospective Studies , Treatment Outcome , Uterine Cervical Neoplasms/pathologyABSTRACT
OBJECTIVES: To analyze the outcomes of pregnancies and risk factors in Chinese women with different stages of systemic lupus erythematosus (SLE). MATERIAL AND METHODS: A total of 55 conceptions in 52 patients with SLE between Jan 2007 and Jan 2019 were retrospected systematically from a general hospital graded 3A in China. Medical records provided us a good way to retrieve the clinical parameters and lab data of patients. RESULTS: Pregnant women with SLE activity had significant hyperimmunoglobulin, hypocomplement, low platelet counts, high erythrocyte sedimentation rate, C-reactive protein and 24-h urine protein. Hydroxychloroquine had been used to reduce the rates of SLE activity in pregnant women. Logistic regression analysis showed low platelet counts, hypocomplement and 24-h urine protein were significantly correlated with fetal loss. Compared to those in stable stage, the active SLE patients have more risks of hypertensive disorders of pregnancy, thrombocytopenia, lupus nephritis and placental infarction, and have worse fetal outcomes, including the higher rate of fetal loss, preterm and asphyxia neonatorum. CONCLUSIONS: Different stages of SLE during pregnancy are closely related to maternal and fetal outcomes. It is imperative to provide SLE women with pregnancy consultation and regular multispecialty care.
