ABSTRACT
The present study explored the effect of miR-200b on the development of diabetic retinopathy (DR) by targeting vascular endothelial growth factor A (VEGFA) gene. The study populations consisted of 255 DR patients (case group) and 253 healthy people (control group), while the expressions of miR-200b and VEGFA mRNA were detected by quantitative real-time PCR (qRT-PCR). Bioinformatics software and dual-luciferase reporter assay were used to confirm VEGFA as a target gene of miR-200b Also, a total of 70 Wistar male rats were selected and randomly assigned into blank, normal control (NC), miR-200b mimics, miR-200b inhibitors, miR-200b inhibitors + silencing vascular endothelial growth factor A (siVEGFA), and siVEGFA groups (n=10/group) respectively. Streptozotocin (STZ)-induced rat models of DR were successfully established. VEGFA, transforming growth factor-ß1 (TGF-ß1), hepatocyte growth factor (HGF), and pigment epithelium-derived factor (PEDF) were detected using qRT-PCR and Western blotting. In comparison with the control group, the case group showed lower expression of miR-200b but higher expression of VEGFA mRNA. VEGFA was confirmed as a target gene of miR-200b Rats in the miR-200b mimics and siVEGFA groups exhibited higher expression of PEDF mRNA and protein but lower expressions of VEGFA, TGF-ß1, HGF protein, and mRNA than the NC group. There was no remarkable difference in expressions of PEDF, VEGFA, TGF-ß1, HGF protein, and mRNA between the miR-200b inhibitors + siVEGFA and NC groups. In conclusion, the present study demonstrated that miR-200b might alleviate DR development by down-regulating its target gene VEGFA.
Subject(s)
Diabetic Retinopathy/genetics , Gene Expression Regulation , MicroRNAs/genetics , Vascular Endothelial Growth Factor A/genetics , Aged , Animals , Blotting, Western , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Experimental/genetics , Diabetes Mellitus, Experimental/metabolism , Diabetic Retinopathy/etiology , Eye Proteins/genetics , Eye Proteins/metabolism , Female , Hepatocyte Growth Factor/genetics , Hepatocyte Growth Factor/metabolism , Humans , Male , Middle Aged , Nerve Growth Factors/genetics , Nerve Growth Factors/metabolism , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Serpins/genetics , Serpins/metabolism , Transforming Growth Factor beta1/genetics , Transforming Growth Factor beta1/metabolism , Vascular Endothelial Growth Factor A/metabolismABSTRACT
Vibrio alginolyticus is a facultative anaerobic gram-negative bacillus found in normal marine flora. Ocular infections induced by V. alginolyticus are extremely rare. We report a case of endophthalmitis caused by V. alginolyticus to draw attention to V. alginolyticus infections following ocular injuries.
