African swine fever virus structural protein p17 inhibits IRF3 activation by recruiting host protein PR65A and inducing apoptotic degradation of STING.

African swine fever virus (ASFV) is notoriously known for evolving strategies to modulate IFN signaling. Despite lots of efforts, the underlying mechanisms have remained incompletely understood. This study concerns the regulatory role of viral inner membrane protein p17. We found that the ASFV p17 shows a preferential interaction with cGAS-STING-IRF3 pathway, but not the RIG-I-MAVS-NF-κB signaling, and can inhibit both poly(I:C)- and poly(A:T)-induced activation of IRF3, leading to attenuation of IFN-ß induction. Mechanistically, p17 interacts with STING and IRF3 and recruits host scaffold protein PR65A, a subunit of cellular phosphatase PP2A, to down-regulate the level of p-IRF3. Also, p17 targets STING for partial degradation via induction of cellular apoptosis that consequently inhibits activation of both p-TBK1 and p-IRF3. Thus, our findings reveal novel regulatory mechanisms for p17 modulation of IFN signaling and shed light on the intricate interplay between ASFV proteins and host immunity.

Precipitable Water Vapour Retrieval from GPS Precise Point Positioning and NCEP CFSv2 Dataset during Typhoon Events.

Radiosonde is extensively used for understanding meteorological parameters in the vertical direction. Four typhoon events, including three landfalls (MERANTI, NEPARTAK, and MEGI) and one non-landfall (MALAKAS), were chosen in analysing the precipitable water vapour (PWV) characteristics in this study. The spatial distribution of the three radiosonde stations in Zhejiang province does not meet the requirement in analysing changes in PWV during typhoon event. Global position system (GPS) observations are an alternative method for deriving the PWV. This enables improvements in the temporal⁻spatial resolution of PWV computed by the radiosonde measurements. The National Centers for Environmental Prediction (NCEP) re-analysed data were employed for interpolating temperature and atmosphere pressure at the GPS antennas height. The PWV computed from GPS observations and NCEP re-analysed data were then compared with the true PWV. The maximum difference of radiosonde and GPS PWV was not more than 30 mm at Taiz station. The Root-Mean-Square (RMS) of PWV differences between radiosonde and GPS was not more than 5 mm in January, February, March, November, and December. It was slightly greater than 5 mm in April. High RMS in May, June, July, August, September, and October implies that differences in GPS and radiosonde PWVs are evident in these months. Correlation coefficients of GPS and radiosonde PWVs were more than 0.9, indicating that the changes in GPS and radiosonde PWVs are similar. Radiosonde calculated PWVs were used for GPS PWV calibration for understanding the PWV changes during the period of a typhoon event. The results from three landfall typhoons show that the average PWV over Zhejiang province is increasing and approaching China mainland. In contrast, MALAKAS did not make landfall and shows a decreasing PWV trend, although it was heading to China mainland. Generally, the PWV change can be used to predict whether the typhoon will make landfall in these cases. PWV spatial distribution of MERANTI shows that PWV peaks change along the typhoon epicenter over Zhejiang province.

Influence of needling the foot-yangming points on intracellular Ca2+ concentration in smooth muscles of the gastric antrum in rabbits.

OBJECTIVE: To investigate the influence of acupuncture at the points of Foot-Yangming Meridian on intracellular concentration of Ca2, called the 2nd messenger of gastric smooth muscles. METHODS: 45 rabbits were randomly divided into the following 5 groups: a normal saline group, a model group treated with atropine, an acupuncture group treated by needling the points of Foot-Yangming Meridian, an acupuncture group treated by needling the points of Foot-Shaoyang Meridian, an acupuncture group treated by needling the points of Foot-Taiyang Meridian, i.e. 9 rabbits in each group. After treatment, the smooth muscles of the gastric antrum were taken to make the suspension containing alive single muscular cells, and the intracellular calcium concentration ([Ca2+]i) was determined by a spectrofluorometer. RESULTS: The concentration of [Ca2+]i in the group of Foot-Yangming Meridian was obviously higher than that of the atropine group (P<0.01), but with no significant differences found among all the other groups (P>0.05). CONCLUSION: The influence of acupuncture at the points of Foot-Yangming Meridian on gastric movement is related to the release of intracellular Ca2+ in the gastric smooth muscles.