Application prospect of computer vision technology assisted ultrasonography in diagnosis and treatment of liver diseases / 中华消化外科杂志

Ultrasound examination has the advantages of non-radiation, non-invasive, low cost and high efficiency, and is the most commonly used method of liver imaging examination. In recent years, the application of computer vision technology to the intelligent analysis of ultrasound images has become a research hotspot in the field of intelligent healthcare. Through large-scale data training, the intelligent analysis model of ultrasound omics based on machine learning algorithm can assist clinical diagnosis and therapy, and improve the efficiency and accuracy of diagnosis. Based on the literature, the authors summarize the application proprect of computer vision technology assisted ultrasonography in the evaluation of diffuse liver lesions, focal liver lesions, microvascular invasion of liver cancer, postoperative recurrence of liver cancer, and postoperative therapy response to trans-catheter arterial chemoembolization.

Design, synthesis, and evaluation of aryl-thioether ruthenium polypyridine complexes: A multi-target antimicrobial agents against gram-positive bacteria.

With the increase in bacterial resistance, new antimicrobial agents are urgently need for developing to combat multidrug-resistant pathogens and with low cytotoxicity. In this study, four new ruthenium polypyridine complexes bearing 4-tBu-phenyl sulfide Ru(bpy)2(TBPIP)](PF6)2(Ru(Ⅱ)-1), Ru(dmb)2(TBPIP)](PF6)2(Ru(Ⅱ)-2), Ru(dmob)2(TBPIP)](PF6)2(Ru(Ⅱ)-3) and Ru(dtb)2(TBPIP)](PF6)2(Ru(Ⅱ)-4) were designed, synthesized and evaluated. Those ruthenium complexes showed strong activity against Staphylococcus aureus (S. aureus) in vitro and in vivo. The Ru(Ⅱ)-1 showed excellent antimicrobial activity against Gram-positive bacteria (MIC = 2.0 µg/mL), poor hemolytic activity (HC50 > 200 µg/mL), and low cytotoxicity to mammalian cells. Ru(Ⅱ)-1 can kill bacteria quickly by destroying the bacterial membranes and avoid developing bacterial cross-resistance. Moreover, antibacterial mechanism studies show that Ru(Ⅱ)-1 destroys the integrity of bacterial cell membrane by permeabilization and depolarization of bacterial cell membrane, and interacts with bacterial DNA to produce a large number of ROS to kill bacteria. Importantly, Ru(Ⅱ)-1 exhibited effective in vivo efficacy in the mouse S. aureus infection model. These results indicated that ruthenium polypyridine complexes modified with 4-tBu-phenyl sulfide had the therapeutic potential as a novel membrane-active antimicrobial to combat Gram-positive bacterial infections.