Efficacy of stimulating Mingmen (GV4) and Guanyuan (CV4) on kidney deficiency in rat model: laser irradiation traditional moxibustion.

OBJECTIVE: To investigate the effect of 10.6-µm laser moxibustion (LM) and traditional moxibustion (TM) on kidney deficiency (KYD) model rats. METHODS: Forty rats were randomly divided into normal, model, 10.6-µm LM, and TM groups. KYD was induced through intramuscular hydrocortisone injections. RESULTS: Hydrocortisone injections decreased the body weight, activity, and temperature and induced changes in the organ coefficients. Compared with the model group, TM and LM treatment prevented body weight loss and improved organ coefficients after treatment ( < 0.05), while the body weight remained lower compared to the normal group ( < 0.01). Additionally, LM and TM significantly increased the levels of urine 17-hydroxycorticosteroid (17-OHCS) compared with the model group ( < 0.01). The LM and TM groups showed a greater distance travelled across the central area, time spent in the central area, and 5-min total distance than the model group in the open field test ( < 0.01). The model group had the lowest anal or surface temperature ( < 0.05); there were no differences among the other three groups ( > 0.05). TM treatment increased the temperature of Mingmen (GV4) and Guanyuan (CV4) compared to the model group ( < 0.05, < 0.01). LM treatment increased Mingmen (GV4) temperature ( < 0.05), while there were no obvious differences in Guanyuan (CV4) temperature compared with the normal group before and after treatment ( > 0.05). There was a positive correlation between Mingmen (GV4)/Guanyuan (CV4) temperature and 17-OHCS levels. CONCLUSIONS: LM and TM treatment can improve KYD symptoms, and acupoint temperature changes may be an objective indicator of KYD.

Prevalence of obstructive sleep apnea-hypopnea syndrome in adults aged over 60 yeaes in dongying city / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To survey the prevalence of obstructive sleep apnea-hypopnea syndrome(OSAHS) in adults aged over 60 years in Dongying city,and analyze the risk factors and the extent of damage to the quality of life for the elderly to provide the basis for prevention and treatment of OSAHS people.@*METHOD@#One thousand subjects were derived from a random cluster sampling in seven districts of Dongying city:they were asked to answer the questions from questionnaires. According to the questionnaire scoring, 100 subjects in high-risk group were selected randomly to make polysomnography monitoring for a whole night,so that the prevalence of the disease was calculated and the related risk factors were analyzed; elderly patients diagnosed with OSAHS were asked to assess the quality of life assessment questionnaire by face to face to understand the quality of life dimensions injury.@*RESULT@#The actual number of completed surveys was 934, and the efficiency was 93.4%. The estimated prevalence of OSARS in elder people defined by apnea-hypopnea index (AHI ≥ 5) was 32.5%; Multivariate analysis revealed that age smoking, family snoring,neck circumference, waist circumference, and abnormality of the upper airway were respectively independent risk factors of OSAHS,and the abnormalities of the upper airway had the most obvious impact on AHI. The damage caused by OSAHS to the quality of life for elderly people followed their daily work life, social relationships, symptoms, alertness, emotional, general health, symptoms.@*CONCLUSION@#The estimated prevalences of OSAHS in elder people were high. Actively promoting good habits to older people, weight loss, early detection and correct upper airway abnormalities may reduce the estimated prevalence of OSAHS. In the treatment process, the patient's physician should pay attention to their emotional and groom their psychological problems to improve the quality of life in elder people.

Expression of PHH3 and cyclinD1 and their clinical significance in breast invasive ductal carcinoma / 中国基层医药

Objective To investigate the association between expression of PHH3 and CyclinD1 and their significance in breast invasive ductal carcinoma.Methods Immunostoemical MaxVision staining was used to detect the expression of PHH3 and CyclinD1 in 89 samples of breast invasive ductal carcinoma and 30 samples of normal breast tissues.The correlation was then analyzed between the expression of PHH3 and CyclinD1 and the clinical path-ological parameters as well as prognosis.Results The positive rate of PHH3 in breast invasive ductal carcinoma tis-sues(68 /89,76.4%)was significantly higher than that in normal breast tissues(2 /30,6.7%)(χ2 =45.051,P 0.05 )and the tumor diameter(χ2 =0.014,P >0.05 ).The positive rate of CyclinD1 in breast invasive ductal carcinoma tissues(62 /89,69.7%)was significantly higher than that in normal breast tissues(3 /10,10.0%)(χ2 =32.223,P 0.05)and the tumor diameter (χ2 =1.836,P >0.05).The expression of PHH3 was directly correlated to the CyclinD1 (r =0.497,P <0.05). Kaplan-Meier analysis showed that the expression of PHH3 was negatively correlated with prognosis.Log-Rank test indicated that there was significant difference(χ2 =10.468,P <0.05),CyclinD1 was negatively correlated with prog-nosis.Log-Rank test indicated that there was significant difference (χ2 =7.906,P <0.05 ).Conclusion High expression of PHH3 and CyclinD1 may be correlated with evolution and metastasis of breast invasive ductal carcino-ma.Combined analysis of PHH3 and CyclinD1 may provide a theoretical basis for prognostic information and treatment of patients with breast cancer.

[Effects and activated mechanism of adenosine triphosphate on BK channel of coronary smooth muscle cells].

OBJECTIVE: To explore the effects and activation mechanism of adenosine triphosphate (ATP) on large conductance calcium-activated potassium channel ( BK channel) in coronary artery smooth muscle cells. METHODS: Coronary smooth muscle cells were isolated by enzyme digestion from Sprague-Dawley rats. And BK currents were recorded by patch clamp technique in whole cell configuration. The effects of ATP on cytosolic calcium concentrations were examined by recording the changes of fluorescence intensity ratios. RESULTS: BK current densities were (137 ± 13) pA/pF and (179 ± 15) pA/pF before and after a perfusion of 1 mmol/L ATP (P < 0.05). The fluorescence ratios were 2.46 ± 0.08 and 4.04 ± 0.21 (P < 0.05) before and after 0.5 mmol/L perfusion. After incubating with purine receptor (P2Y1) blocker MRS2179, phospholipase C (PLC) blocker U73122 and inositol triphosphate (IP3) blocker 2-APB, the fluorescence ratios were 2.7 ± 0.06, 2.65 ± 0.12 and 2.69 ± 0.13 respectively. Compared with control group, all fluorescence ratios decreased after incubating with three blockers (P < 0.05). CONCLUSION: ATP may elevate intracellular calcium concentration via P2Y1-PLC-IP3 pathway consequently activating BK channel.

Effect of Yatongning tincture root canal implantation on IL-8,CD4 and HSP70 levels in gingival crevicular fluid of patients with periodontitis / 中国生化药物杂志

Objective To explore the effect of Yatongning tincture root canal implantation on interleukin 8 ( IL-8 ) , cluster of differentiation 4 (CD4) and heat shock protein 70(HSP70) in in gingival crevicular fluid of patients with periodontitis.Methods 64 patients with periodontitis from December 2013 to September 2015 in stomatology department of Yuyao Second People’s Hospital were selected and according to the different treatment methods divided into treatment group and control group, 33 cases in treatment group, 31 cases in control group.Patients received root canal implantation in two groups, the treatment group received Yatongning tincture root canal implantation on the basis of control group.The clinical curative effect, incidence of pain, IL-8, CD4 and HSP70 levels were compared between two groups.Results The clinical curative effect of treatment group was 96.97%(32 cases), which was higher than 80.65% (31 cases) of control group (χ2 =4.373, P<0.05).The pain incidence of treatment group was 30.30%(10 cases), which was higher than 58.06% (18 cases) of control group (χ2 =5.006,P<0.05).After treatment, the IL -8 levels were higher and CD4, HSP70 levels were lower in two groups than those pre-treatment(P<0.05), compared with control group, the IL-8 levels were higher and CD4, HSP70 levels were lower in treatment group ( P<0.05 ) .Conclusion Yatongning tincture root canal implantation could enhance the clinical efficacy , relieve tooth tissue damage and improve the post-operative pain in patients.

Association of glycosylated hemoglobin and serum C-reactive protein in type 2 diabetes patients with periodonti-tis / 实用口腔医学杂志

Serum high sensitivity C-reactive protein(hs-CRP)and glycosylated hemoglobin(HbA1c )were detected in 89 type 2 diabetes patients with chronic periodontitis.The patients were divided into HbA1c≥ 7.0% and <7.0% groups according HbA1c levels.Serum hs-CRP,probing depth(PD),attachment loss(AL)in HbA1c ≥ 7.0% group were all significantly higher than those in <7.0% group (P <0.01 ),and serum hs-CRP was positively correlation with PD and AL in all patients.In patients with type 2 diabetes,high HbA1c and chron-ic periodontitis serum hs-CRP is positively correlation with periodontal disease.

Clinical Observation on Treatment of Adult Serious Chronic Periodontitis with Periocline Ointement as Auxiliary Therapy / 昆明医科大学学报

Objctive To evaluate therapeutic effect of periocline ointment as an auxiliary treatment of adult serious chronic periodontitis. Methods 56 patients were divided into the test group and the control group in register sequence. Following initial therapy, patients in the test group were treated with PERIOCLINE into the periodontal pocket once a week for 4 weeks, while patients in the control group were treated with iodine glycerine. At the baseline level, the indexes of PLI,SBI,PD,AL were recorded and compared between the two groups on the 1st month, 3rd month and 6th month. Results All the indices of both groups were improved, compared with the baseline ( <0.05) . The results of the test group were better than those of the control group, and the differences were statistically significant (<0.05) . Conclusion Periocline ointment as an auxiliary treatment of adult serious chronic periodontitis is superior to compounded iodine glycerine in the amelioration of all the indices.

Radiolabeling of Filamentous Phage Peptide Library With 99mTc and Its Biodistribution in Normal Mice / 生物化学与生物物理进展

Numerous peptides that bind to a given target have been selected by phage display technology. However, some peptides isolated to date do not bind with high affinity to tumor or organ sites, even peptides were selected in vivo. Therefore, the biodistribution of 99mTc-labeled filamentous phage peptide library via MAG3 (mercaptoacetyltriglycine) were investigated to gain a better understanding of phage circulation in vivo. The experimental results showed that the liver and spleen were the organs of the greatest accumulation, while heart, muscle, pancreas and brain retained less radioactivity. In opposite to other tissues and organs, the radioactivity in stomach, intestine and bone gradually went up with time. The clearance of 99mTc-labeled phage in blood was very fast from 5 min to 30 min and then slowed down. When phage in vivo circulated at enough long period of time, some phage particles could extravasate in some organs or tissues and internalized there. In conclusion, the circulation time of phage in vivo should be experimentally determined beforehand according to the targeted organs and the specific location of target peptides in order to panning a peptide with high specificity and affinity to that target.

Evaluation of the cytotoxicity of four dental alloys on human gingival fibroblasts / 实用口腔医学杂志

Objective:To identify the cytotoxicity of four dental casting alloys on human gingival fibroblasts.Methods: MIT assay method was used to evaluate the cytotoxicity of four alloys on human periodontal ligament fibroblasts(HPDLFs) isolated from healthy periodontal ligaments of subjects on the 2nd d,4th d and 7th d of culture.Flow Cytometry(FCM) was used to evaluate the cytotoxicity of four alloys to human gingival fibroblasts.Results: It was found that there was no apparent cytotoxicity of all the four alloys.Conclusion:MTT and FCM methods show the same results.These four alloys have good biocompatibilities.

THE STUDY OF HUMAN PARVOVIRUS B19 INFECTION IN PERINATAL TRANSMISSION AND ABNORMAL FETUSES AND NEONATES IN GUANGDONG / 中国人兽共患病学报

This study was undertaken to investigate mother-to-infant transmission of human parvovirus B19 and the significance of prevalence of B19 virus in abnormal fetuses in Guandong. The polymerase chain reaction (PCR)was established to detect parvovirus B19 DNA in 700 sera from 350 maternal-infant pair groups. The prevalence of B19 virus DNA was 1.14％ (4/350)and 0.28％(1/350)in the sera of pregnant women and cord blood of their neonates respectively. Parvovirus B19 DNA sequences were also detected in abnormal fetuses and new-born by PCR. The positive results were obtained in 5 samples of fetal tissues from 17 abnormal fetuses and in 3 those of neonatal tissues from 7 cases of neonatal death. The amplified products of PCR were identified to be the target DNA with Hae Ⅲ digestion. By in situ hybridization ,parvovirus DNA could be detected mainly in the nuclei of immature hematopoetic cells within fetal brain or spleen whose PCR tests were positive. The study results suggest that human parvovirus B19 infection does exist in maternal-infant transmission in Guangdong and might lead to harm on fetuses,but the prevalence rate of B19 virus may be very low. The evaluation of B19 virus infection might depend on reliable assay to determine present infection or past infection.