ABSTRACT
Breast cancer is the most common malignant tumor in women and remains a major global challenge, with ~1.4 million cases per year, worldwide. Numerous studies have shown that changes in cell metabolism are associated with the regulation of tumor progression. In the present study, the anti-cancer properties of glyoxal (GO), which is the smallest dialdehyde formed in the oxidation-reduction reaction and involved in electron transfer and energy metabolism, in breast cancer was investigated. The biological functions and molecular mechanisms of GO were investigated in breast cancer cell lines using MTT and crystal violet assays, flow cytometry, western blot analysis, 3D laser scanning confocal microscopy and transmission electron microscopy. The results showed that GO strongly inhibited cell proliferation, promoted cell apoptosis and cell cycle G2/M arrest, induced the disappearance of cellular microvilli, and enlarged mitochondria. In addition, the protein expression level of AKT, mTOR and p70-S6K decreased in the AKT-mTOR pathway, accompanied by an increase in p-ERK and p-MEK in the MAPK pathway. The results from the present study indicate that GO suppressed breast cancer progression via the MAPK and AKT-mTOR pathways. Taken together, these results provide the basis for a potential therapeutic strategy for breast cancer.
ABSTRACT
OBJECTIVE:To observe the effects of Compound biejia ruangan tablets combined Entecavir tablets on liver func-tion and hematoidin of patients with chronic hepatitis B(CHB)blood stasis syndrome. METHODS:Totally 110 patients with CHB blood stasis syndrome were selected from Neijiang Second People's Hospital,the Affiliated Hospital of Southwest Medical Univer-sity and Yibin First People's Hospital during Jan.-Dec. 2015. They were divided into control group and observation group by strati-fied blocked randomitation,with 55 cases in each group. Control group was given Entecavir tablets 0.5 mg,po,qd;observation group was additionally given Compound biejia ruangan tablets 2.0 g,po,tid. Both groups received treatment for 12 months. TCM syndrome scores,the levels of liver function indexes(AST,ALT,ALP,GGT),serum bilirubin(TBIL,DBIL,IBIL),liver fi-brosis indexes(LN,HA,PⅢP,Ⅳ-C)and Fibroscan(FS)were observed in 2 groups before and after treatment. The occurrence of ADR was recorded. RESULTS:In observation group,2 patients withdrew from the study,and 3 patients of control group with-drew from the study. Before treatment,there was no statistical significance in TCM syndrome scores,the levels of liver function in-dexes,serum bilirubin,liver fibrosis indexes and FS between 2 groups(P>0.05). After treatment,TCM syndrome scores,the lev-els of TBIL,IBIL,LN,HA,PⅢP,Ⅳ-C and FS in 2 groups were significantly decreased,while DBIL level was increased signifi-cantly,and the observation group was significantly better than control group,with statistical significance(P<0.05). The levels of AST and ALT in observation group were decreased significantly,while the levels of ALP and GGT were increased significantly, and the observation group was significantly better than control group,with statistical significance(P<0.05). There was no statisti-prove liver function indexes and bilirubin level,and inhibit liver fibrosis with good safety.
ABSTRACT
Objective To understand the epidemiological and clinical features of Brucella infection outbreak, provide basis for the timely diagnosis, prevention and control of brucellosis.Methods Epidemiological history, clinical symptoms and signs, auxiliary examination, and therapeutic efficacy of 8 patients with brucellosis and admitted to a hospital between December 2015 and April 2016 were analyzed retrospectively.Results All 8 patients had touched stray sheep and participated in the butchering, all were male patients, with the average age of 50 years;all patients had sweating, fatigue and other symptoms, 7 had a fever, 6 were with lumbago, 3 with muscular soreness, and 2 had a cough.Brucella was cultivated from blood specimens of 8 patients, the specific antibody titers ranged from 1:200 to 1:1 600.All patients recovered from illness through 6-week treatment with rifampin plus levofloxacin, without recurrence after 6-month observation.Conclusion Clinical manifestations of brucellosis are atypical, history of epidemiology should be carefully inquired, combined with etiology, early diagnosis and early treatment can be performed.
