ABSTRACT
OBJECTIVE: To investigate the chemical constituents of active fraction of Cynanchum versicolor. METHODS: The ethanol extract of Cynanchum versicolor was purified by macroporous resin, silica gel column and Sephadex LH-20 column chromatography, and structures of the isolated compounds were identified by spectroscopic analysis and comparison with those of literatures. RESULTS: Six compounds were isolated and identified as glaucogenin C (I), 24-methyl-5α-cholesta-7,22-diene-3ß,5,6ß-triol (II), syringic acid (III), 3,4-dihydroxyacetophenone (IV), 4-hydroxyacetophenone (V), and 4-hyroxy-3-methoxyacetophenone (VI). CONCLUSION: Compounds I, II and IV are isolated from this plant for the first time.
Subject(s)
Cynanchum/chemistry , Phytochemicals/isolation & purification , Acetophenones/chemistry , Acetophenones/isolation & purification , Gallic Acid/analogs & derivatives , Gallic Acid/chemistry , Gallic Acid/isolation & purification , Phytochemicals/chemistryABSTRACT
OBJECTIVE: To study the dynamic change law of volatile oil, saikosaponin a, d and alcohol-extract from Bupleurum chinense at Songxian region in Henan province, and to explore the optimal harvest period of Bupleurum chinense. METHODS: With the contents of saikosaponin a and d, absorbance of volatile oil and percentage of alcohol-extract as indexes, HPLC-ELSD and ultraviolet spectrophotometry were successively used to analyze them. RESULTS: There are obvious differences among the contents of volatile oil, saikosaponin a, d and alcohol-extract in various collecting periods sample, the absorption of volatile oil in distillation was the highest in October, the content of saikosaponin a was the highest in September, the saikosaponin d in December and the percentage of alcohol-extract in October. CONCLUSION: The optimal harvest period of Bupleurum chinense at Songxian region in Henan is identified, which can provide scientific basis for crude drug production and processing.
