Mismatch repair gene MSH6 correlates with the prognosis, immune status and immune checkpoint inhibitors response of endometrial cancer.

Objective: Many patients treated with immune checkpoint inhibitors (ICIs) developed primary or secondary drug resistance for unknown reasons. This study investigates whether mismatch repair (MMR) genes are responsible for this therapeutic restriction. Methods: We obtained the transcriptional, clinical and single nucleotide polymorphism data for endometrial cancer (EC) from The Cancer Genome Atlas and the immunophenoscore data of EC from The Cancer Immunome Atlas, then analyzed in R to evaluate the relationship between MMR genes and clinicopathological features, prognosis, immune infiltration, immune checkpoint expression and responsiveness to ICIs in EC. We used differentially expressed genes in the MSH6 high and low expression groups to conduct GO and KEGG analyses to explore the impact of MSH6 on the biological functions of EC. Finally, we verified the bioinformatics results with in vitro experiments. Results: Our analyses showed that compared with the high MSH6 expression group, the low MSH6 expression group had better survival outcomes and less aggressive clinicopathological features. In the multivariate Cox analysis, MSH6 was the only independent risk factor that could predict the prognosis of EC. Besides, the low MSH6 expression group also had a higher immune score, more active immune infiltration and higher immune checkpoint expression, resulting in better responsiveness to ICIs treatment, consistent with the enrichment of GO terms and KEGG pathways related to immune response in this group. Meanwhile, the GO and KEGG enrichment results of the MSH6 high expression group were associated with cell cycle, DNA damage repair and tumorigenesis. To exclude the influence of MSH6 mutations, we performed the previous analyses on the MSH6 wild-type tumor samples and obtained consistent results. In vitro experiments also confirmed that after knocking down MSH6 in endometrial cancer cells, their proliferation, migration and invasion abilities were weakened, while the expression levels of PD-L1 and PD-L2 were elevated. In comparison, overexpression of MSH6 showed an opposite trend. Conclusion: Reduced MSH6 expression could serve as a potential biomarker for predicting better prognosis, active immune status, higher immune checkpoint expression level and better responsiveness to ICIs treatment in EC. MSH6 may become a potential target for treating solid tumors.

miR-152 is involved in the proliferation and metastasis of ovarian cancer through repression of ERBB3.

MicroRNAs (miRNAs) participate in post-transcriptional regulation by targeting the 3' untranslated region of target genes that are involved in diverse biological processes. To the best of our knowledge, the association between miR­152 and ERBB3 in ovarian cancer remains unclear. In the present study, a negative correlation between miR­152 and ERBB3 in ovarian cancer was observed. The luciferase reporter gene assay results demonstrated that miR­152 negatively regulated ERBB3 in SKOV3 and OVCAR3 ovarian cancer cells. Furthermore, our results revealed that miR­152 suppressed the ability of ovarian cancer cell proliferation, migration and invasion, and promoted apoptosis through inhibiting ERBB3 in vitro. Therefore, in the present study, miR­152 was found to be involved in the proliferation and metastasis of ovarian cancer cells through repression of ERBB3 expression. Therefore, miR­152 may be a potential therapeutic target for the treatment of ovarian cancer.

Investigation of dry eyes occurrence and poor vision in primary and middle school students in the urban-rural fringe of Guangzhou / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To investigate the relationship between dry eyes incidence and poor vision in primary and middle school students in the urban-rural fringe of Guangzhou. <p>METHODS: We used stratified cluster random sampling, extracted the urban-rural fringe elementary school and middle school, gave out self-made questionnaire survey to 3572 students, at the same time, underwent ophthalmology routine inspection and tear secretion test, rupture time measurement and corneal and conjunctival fluorescein staining. <p>RESULTS: The overall number of poor vision was 764 people(1447 eyes), the overall poor vision was 20.25% counted by eye amount, the rate of poor vision in primary school was 14.08%, in junior high school was 29.51%, in junior middle school was significantly higher than that in primary school(<i>P</i><0.05). The number of subjects with dry eyes was 424 cases(848 eyes), the overall incidence of dry eyes was 11.87%, among them the occurring rate of dry eyes in primary school was 5.14%, in junior middle school was 21.96%, which was significantly higher than that in primary school(<i>P</i><0.05). Ordered multivariate Logistic regression analysis showed that junior high school, doing homework for 1-3h or >3h every day, eyes away from the book <30cm, lying down when reading books, watching TV or playing computer phone for 1-3h and >3h every day, dry eyes, and the nutritional status(fat or thin)were the risk factors of poor vision in primary and middle school students the(<i>OR</i>=3.862, 1.164, 1.210, 1.212, 1.315, 1.319, 3.040, 1.110, 1.057 and 1.141). <p>CONCLUSION: The risk factors of primary and middle school students vision is multifarious, dry eyes is a risk factor for poor vision of primary and middle school students in the urban-rural fringe of Guangzhou.

Effect of LY294002 on adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells / 中国医学科学院学报

<p><b>OBJECTIVE</b>To study the effect of LY294002 on the adriamycin- induced epithelial-mesenchymal transition in human breast carcinoma cells.</p><p><b>METHODS</b>Human breast carcinoma cells MCF-7 was cultured in vitro and then exposed to adriamycin with or without LY294002. The protein expression levels of Akt, phosphorylated-Akt (p-Akt), Snail, and E-cadherin was detected by Western blot analysis. The mRNA expressions of Snail and E-cadherin were determined by RT-PCR.</p><p><b>RESULTS</b>Adriamycin significantly increased the protein expression of Snail and depressed the protein expression of E-cadherin (P<0.05). The pre-treatment with LY294002 significantly reversed the changes of activities and levels of the above proteins (P<0.05).</p><p><b>CONCLUSION</b>LY294002 could reverse the adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells by regulating the expressions of Snail and E-cadherin through suppressing PI3K/Akt signaling pathway.</p>

Effects of Using Topiramate Combined with Traditional Chinese Herbal Medicine to Treat Tourette's Syndrome in Children / 实用儿科临床杂志

0.05].After 12 week treatment,the YGTSS total score in study group was significantly lower [(27.88?14.24) vs(36.53?17.45) scores] and the reduction rate of YGTSS score was significantly higher [(43.12?18.42)% vs(33.49?14.38)%] compared with those in control group(Pa

Influence of Gestational Impaired Glucose Tolerance of Pregnancies on Umbilical Adiponectin Level of Newborns / 实用儿科临床杂志

Objective To investigate the changes of umbilical adiponectin(APN) levels in newborn whose mothers with gestational impaired glucose tolerance (GIGT).Methods Eighteen neonates of GIGT pregnancies and 50 controls of normal glucose tolerance (NGT) pregnancies were studied.APN concentration was qualified in the umbilical vein serum with radioimmunoassay methods.Capillary blood glucose was measured at the first hour after delivery.Results Umbilical APN level in the GIGT group was significantly higher than that in NGT group [(61.06?16.85)mg/L vs(48.04?15.47)mg/L,t=2.38 P0.05).Conclusions Umbilical serum APN levels are elevated in newborns of GIGT mo-thers.This may be due to the dysregulation of serum APN levels associate with GIGT pregnancy.More attention shall be paid to the metabolic changes of GIGT mothers and their offspring.