Gut microbiome play a crucial role in geographical and interspecies variations in mercury accumulation by fish.

Mercury (Hg) contamination in fish has raised global concerns for decades. The Hg biotransformation can be manipulated by gut microbiome and it is found to have a substantial impact on the speciation and final fate of Hg in fish. However, the contribution of intestinal microbiota in geographical and interspecies variations in fish Hg levels has not been thoroughly understood. The present study compared the Hg levels in wild marine fish captured from two distinct regions in South China sea. We observed a quite "ironic" phenomenon that MeHg levels in carnivorous fish from a region with minimal human impacts (Xisha Islands, 92 ± 7.2 ng g-1 FW) were much higher than those from a region with severe human impacts (Daya Bay, 19 ± 0.41 ng g-1 FW). Furthermore, the results showed that gut microbiome determined Hg biotransformation and played a crucial role in the variances in fish Hg levels across different geographical locations and species. The intestinal methylators, rather than demethylators, were more significant in affecting Hg biotransformation in fish. The carnivorous species in Xisha Islands exhibited a higher abundance of intestinal methylators, leading to higher MeHg accumulation. Besides, the gut microbiome could be shaped in response to the elevated Hg levels in these fish, which may benefit their adaptation to Hg toxicity and overall health preservation. However, anthropogenic activities (particularly overfishing) in Daya Bay have severely affected the fish population, disrupting the reciprocal relationships between fish and intestinal microbiota and rendering them more susceptible to pathogenic microbes. Overall, this study provided a comprehensive understanding of the role of gut microbiome in Hg bioaccumulation in fish and offered valuable insights into the co-evolutionary dynamics between fish and gut microbiome in the presence of Hg exposure.

Distribution and co-occurrence networks of the bacterial community in sediment cores from the subtropical Daya Bay, China.

The bacterial community plays an important role in biogeochemical cycles in marine sediment. However, little is known about the vertical profiles and co-occurrence patterns of bacterial community in sediment cores from the marine environment. In this study, five sediment cores were taken from a subtropical bay in China, heavily impacted by anthropogenic activities. The bacterial composition in sediment cores was investigated by using high-throughput sequencing of the 16S rRNA gene. A principal coordinates analysis and an adonis analysis of the operational taxonomic unit (OTU) compositions showed that spatial variation, rather than vertical variation, determined the bacterial structure in sediment cores. The bacterial complexity varied greatly across the five sediment cores, and the rare taxa played an important role in supporting the stability of the bacterial network. This study revealed that sediment properties and anthropogenic activities may induce a shift in the bacterial composition in sediment cores of a subtropical bay.

Effects of diet composition on gut microbiome and mercury biotransformation in the gobyfish.

Mercury (Hg) biotransformation (methylation and demethylation) could play a key role in affecting the final Hg speciation and level in fish. The gut microbiota was identified to be involved in this process. Diet is recognized to greatly influence the gut microbiome, whereas the impact of food composition on Hg biotransformation in fish has yet to be addressed. The study investigated the biotransformation and bioaccumulation of Hg in the gobyfish (Mugilogobius chulae) under different food choices (natural prey and artificial food) and evaluated the role of gut microbiome in these processes. The results showed that different diet composition significantly affected the gut microbiome and subsequently resulted in varied phenomenon of Hg biotransformation within fish body. Significant demethylation (0.33 % d-1) was only observed in the natural prey (brine shrimp) treatment, whereas methylation was occurred extremely slowly (0.013 % d-1) only in the artificial food (commercial dry pellets) treatment. Furthermore, the growth of demethylators was also enhanced in the natural prey treatment, which contributed to the demethylation process in fish. Furthermore, the gut microbial structure of gobyfish was greatly altered by different diet composition. This study highlights the significance of food choices in the minimization of Hg contamination in aquaculture. Incorporating natural prey into fish diets could be a better choice to balance the fish production and control MeHg levels. CAPSULE: Diet composition greatly affects the gut microbial composition, and natural prey could help to reduce the potential of MeHg accumulation by fish.

The Keap1-Nrf2 signaling pathway regulates antioxidant defenses of Ctenopharyngodon idella induced by bacterial infection.

Respiratory burst is a process involving rapid production of reactive oxygen species (ROS) for eliminating invading pathogens. However, excessive ROS production can be fatal to the host organism. The Keap1-Nrf2-ARE (Kelch-like ECH-associated protein 1 [Keap1]; Nuclear factor erythroid-derived 2-like 2 [Nrf2]; Antioxidant responsive element [ARE]) signaling pathway plays an important role in alleviating oxidative stress and preserving cellular homeostasis. However, the role of Keap1 during bacterial infection in fish remains unclear. In this study, we cloned and characterized the Keap1 gene of grass carp (CiKeap1) for the first time. CiKeap1 encodes a 593-amino acid protein of the Keap1b type. The tissue distribution analysis data revealed that the brain contains the highest transcription level of Keap1, followed by the heart and liver. The infection of Aeromonas hydrophila and Staphylococcus aureus obviously modulated the gene transcription and protein expression levels of Keap1, which suggested that the CiKeap1 participates in antibacterial immune responses. Furthermore, in vitro overexpression assays clarified the defensive and regular roles of CiKeap1 in maintaining host redox homeostasis in response to bacterial infection through the Keap1-Nrf2-ARE signaling pathway. In conclusion, the present results provide an expanded perspective on the role of Keap1 in teleost immunology that can guide healthy farming cultivation of grass carp.

Comparative Mitogenome Analyses Uncover Mitogenome Features and Phylogenetic Implications of the Parrotfishes (Perciformes: Scaridae).

In order to investigate the molecular evolution of mitogenomes among the family Scaridae, the complete mitogenome sequences of twelve parrotfish species were determined and compared with those of seven other parrotfish species. The comparative analysis revealed that the general features and organization of the mitogenome were similar among the 19 parrotfish species. The base composition was similar among the parrotfishes, with the exception of the genus Calotomus, which exhibited an unusual negative AT skew in the whole mitogenome. The PCGs showed similar codon usage, and all of them underwent a strong purifying selection. The gene rearrangement typical of the parrotfishes was detected, with the tRNAMet inserted between the tRNAIle and tRNAGln, and the tRNAGln was followed by a putative tRNAMet pseudogene. The parrotfish mitogenomes displayed conserved gene overlaps and secondary structure in most tRNA genes, while the non-coding intergenic spacers varied among species. Phylogenetic analysis based on the thirteen PCGs and two rRNAs strongly supported the hypothesis that the parrotfishes could be subdivided into two clades with distinct ecological adaptations. The early divergence of the sea grass and coral reef clades occurred in the late Oligocene, probably related to the expansion of sea grass habitat. Later diversification within the coral reef clade could be dated back to the Miocene, likely associated with the geomorphology alternation since the closing of the Tethys Ocean. This work provided fundamental molecular data that will be useful for species identification, conservation, and further studies on the evolution of parrotfishes.

The complete mitochondrial genome of Cheilinus trilobatus (Perciformes: Labridae).

Cheilinus trilobatus Lacépède, 1801 is a species of genus Cheilinus. In this study, we sequenced the complete mitochondrion genome of C. trilobatus. The mitochondrial genome was 17,292 bp, consisting of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one non-coding control region (D-loop). The nucleotide composition was 27.31% A, 25.1% T, 17.23% G, and 30.36% C. Phylogenetic analysis suggested that C. trilobatus was closely related to Cheilinus oxycephalus. The complete mitogenome of C. trilobatus provided basic data for the genetic diversity conservation of this species.

Subtypes of Park Use and Self-Reported Psychological Benefits Among Older Adults: A Multilevel Latent Class Analysis Approach.

Healthy aging is a global health priority. Urban parks and green space have been demonstrated to provide mental health benefits to older adults. Despite growing interest in prescribing nature and park visits by physicians, we do not know what type of park visit is most effective for the psychological benefits. This lack of knowledge prevents planners and designers from making informed decisions to promote mental health. We collected field data from 200 visitors from 15 different parks in Shanghai, China. The participants completed pre-visit and post-visit surveys, wearing a GPS and a pedometer while visiting the park. A multilevel latent class analysis (LCA) yielded a three-class structure of park use patterns: the active park lingerer, the active walker, and the passive scanner. Paired-sample t-test and ANCOVA tests showed that affective states (i.e., anxiety, depression, relaxation, contention) were enhanced after park visits for all subtypes. However, the active park lingerer displayed significantly higher levels of relaxation and contention, compared to the active walker and the passive scanner. The findings offer insights into park design characteristics that could promote the mental health of older adults.

Complete mitochondrial genome of the skinnycheek lantern fish Benthosema pterotum (Perciformes: Myctophidae) in the East China Sea.

The complete mitochondrial genome sequence of Benthosema pterotum is first described in this article. The total length of mitogenome is 18,052 bp. It contains 13 protein-coding genes, 22 tRNA genes, and two ribosomal RNA genes. The overall base composition of H-strand is 27.83% A, 30.88% C, 25.61% T, and 15.69% G, with an A＋T bias of 53.43%. The phylogenetic analysis result showed that the B. pterotum and Electrona carlsbergi were close relationship.

[Pollution by heavy metals in the petrochemical sewage waters of the sea area of Daya Bay and assessment on potential ecological risks].

This study aimed to gain a clear understanding on the status of pollution by heavy metals in the petrochemical sewage and the potential ecological risk caused by heavy metal pollution in the sea area of Daya Bay. The contents and spatial distributions of heavy metals including Zn, Pb, Cu, Cd, Cr, As and Hg in seawater, sediment and fishes collected from Daya Bay were analyzed. The comprehensive pollution index (CPI) and ecological risk indexes (ERIs) were used to evaluate the contaminated severity and potential ecological risks of heavy metals in seawater and sediment. The results showed that the contents of these heavy metals, except for those of Zn and Pb, in several stations set in Daya Bay from 2011 to 2012 were relatively low, which were lower than the quality standard of class I according to the China National Standard Criteria for Seawater Quality, suggesting that the seawater in Daya Bay has not been polluted yet by these heavy metals. The average CPI of heavy metals in seawater during flooding season (0.72) was higher than that during dry season (0.38) whereas the average CPI of heavy metals in sediment during dry season (7.77) was higher than that during flooding season (5.70). Hg was found to be the primary contaminating heavy metal in sediment during dry season, which was followed by As and Zn whereas during flooding season, Hg was the primary contaminating metal in sediment, followed by Zn and Cu. The contents of these 7 heavy metals in fishes collected from the surveyed areas were lower than those of the standard requirements. A correlation analysis indicated that there were significant differences in the correlations between the midst of the heavy metals in sea water and the different periods. The ERIs of heavy metals in sediment during dry season (129.20) was higher than that during flooding season (102.86), and 25% of the sampling sites among all stations were under the risk of high-level alarm. The potential ERIs of heavy metals in sediment in offshore waters were higher than those of inshore waters, and were higher in the bay-mouth than in the bay-head. However, the distributions of potential ERIs showed reversed trend during dry season. The comprehensive assessment results showed that Hg was the primary heavy metal with a high ecological risk whereas the potential ERIs for the other six heavy metals in the petrochemical sewage waters in Daya Bay were relatively low.