ABSTRACT
In pig production, reducing production costs and improving immunity are important. Grape pomace, a good agricultural by-product, has been thrown away as food waste for a long time. Recently, we found that it could be used as a new source of pig feed. We investigated the effect of grape pomace on inflammation, gut barrier function, meat quality, and growth performance in finishing pigs. Our results indicated that treatment samples showed a significant decrease in water loss, IL-1ß, DAO, ROS, and MDA content (p < 0.05). IgA, IgG, IgM, CAT, T-AOC, SOD, and IFN-γ significantly increased compared with those in control samples (p < 0.05). Meanwhile, the relative mRNA expression of the tight junction protein occludin showed a significant difference (p < 0.05). Analysis of metagenomic sequencing indicated that grape pomace significantly decreased the relative abundance of Treponema and Streptococcus (p < 0.05). In summary, our results demonstrated that grape pomace could improve meat quality, alleviate inflammation, and decrease oxidative stress.
ABSTRACT
Rhodomyrtus tomentosa is an important fleshy-fruited tree and a well-known medicinal plant of the Myrtaceae family that is widely cultivated in tropical and subtropical areas of the world. However, studies on the evolution and genomic breeding of R. tomentosa were hindered by the lack of a reference genome. Here, we presented a chromosome-level gap-free T2T genome assembly of R. tomentosa using PacBio and ONT long read sequencing. We assembled the genome with size of 470.35 Mb and contig N50 of ~43.80 Mb with 11 pseudochromosomes. A total of 33 382 genes and 239.31 Mb of repetitive sequences were annotated in this genome. Phylogenetic analysis elucidated the independent evolution of R. tomentosa starting from 14.37MYA and shared a recent WGD event with other Myrtaceae species. We identified four major compounds of anthocyanins and their synthetic pathways in R. tomentosa. Comparative genomic and gene expression analysis suggested the coloring and high anthocyanin accumulation in R. tomentosa tends to be determined by the activation of anthocyanin synthesis pathway. The positive selection and up-regulation of MYB transcription factors were the implicit factors in this process. The copy number increase of downstream anthocyanin transport-related OMT and GST gene were also detected in R. tomentosa. Expression analysis and pathway identification enriched the importance of starch degradation, response to stimuli, effect of hormones, and cell wall metabolism during the fleshy fruit development in Myrtaceae. Our genome assembly provided a foundation for investigating the origins and differentiation of Myrtaceae species and accelerated the genetic improvement of R. tomentosa.
ABSTRACT
Seed vigor of rice is an important trait for direct seeding. The objective of this study was to reveal the relationship between globulin and seed vigor, and then to explore a method for evaluating seed vigor. Several rice varieties with different levels of 52 kDa globulin accumulation were used to compare seed vigor under normal and aged conditions. Results showed that varieties with high globulin accumulation obtained significantly higher seed vigor, measured by germination percentage and germination index, compared with those varieties with low globulin accumulation under normal and aged conditions. Meanwhile, a significantly higher accumulation of reactive oxygen species (ROS) was observed in the early germinating seeds of varieties with high globulin accumulation compared to those varieties with low globulin accumulation under normal and aged conditions. Collectively, the globulin content could be applied in the evaluation of seed vigor, which contributes to the selection of rice varieties for direct seeding.
Subject(s)
Globulins , Oryza , Germination , Globulins/genetics , Oryza/genetics , Seeds/geneticsABSTRACT
Artificial insemination (AI) is a proven breeding technology which has been widely used in pig reproduction. Low temperature can cause very serious damage to pig sperm below 15 °C and the situation is even more serious at lower temperature. Besides, the preservation of pig sperm is mainly carried out at 17 °C because of its outstanding performance in pig reproduction. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17 °C, we compared them with the traditional diluent to study the sperm motility, the cumulative amount of ROS, the extent of sperm membrane damage, the sperm acrosome integrity, the sperm DNA damage and the activity of antioxidant enzymes (CAT, T-AOC, SOD, GSH-PX, MDA) to evaluate the effect of hydroxytyrosol on the sperm quality during storage. We used proteomics sequencing technology to monitor difference in sperm protein between the control samples and the addition of 120 µmol/L hydroxytyrosol samples (optimum concentration) after three days storage. Ultimately, we selected the control samples and the addition of 120 µmol/L hydroxytyrosol samples to test the effect of AI. The results of our research showed that during storage of pig sperm at 17 °C, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated samples significantly improved (HT 120 µmol/L) (P < 0.05). Proteomics sequencing analysis proved that the addition of 120 µmol/L hydroxytyrosol treatment samples had potential value in improving sperm quality. The significant increase in sow pregnancy rate and piglet birth weight proved that hydroxytyrosol had important practical value in pig reproduction. Based on our results, we demonstrated that the addition of hydroxytyrosol to the diluent could improve the quality of pig sperm and the efficiency of AI.
