Transplantation of hypoxia preconditioned bone marrow mesenchymal stem cells improves survival of ultra-long random skin flap / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Random flap is one kind of the most widely used skin flaps in reconstructive surgery; however, partial necrosis of its distal end remains a significant problem now. The aim of this study was to evaluate the effect of hypoxia preconditioned bone marrow mesenchymal stem cells (HpBMSCs) transplantation on ultra-long random skin flap survival in rats.</p><p><b>METHODS</b>Normoxic bone marrow mesenchymal stem cells (nBMSCs) were cultured under normoxia (20% O2) and HpBMSCs under hypoxia (1% O2) for 48 hours before transplantation. Thirty Sprague-Dawley rats were randomly divided into control group, nBMSCs group and HpBMSCs group with each consisting of 10 rats. Survival area of ultra-long random skin flap on the dorsal of rats was measured seven days after flap surgery and cell transplantation. Cell survival in vivo, microvessel density and vascular endothelial growth factor (VEGF) were evaluated by histological examination and enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Compared with other two groups, flap survival area in HpBMSCs group was significantly larger (P < 0.05). Microvessel density in HpBMSCs group (36.20 ± 8.19) was higher than that in nBMSCs group (30.01 ± 5.68) and control group (17.60 ± 4.19) (P < 0.05). VEGF in HpBMSCs group ((300.05 ± 50.41) pg/g) was higher than those in nBMSCs group ((240.55 ± 33.64) pg/g) and control group ((191.65 ± 32.58) pg/g) (P < 0.05).</p><p><b>CONCLUSION</b>HpBMSCs transplantation improves ultra-long random skin flap survival via promoting angiogenesis of more survival cells.</p>

In vitro study on expression of chemokine receptor CX3CR1 in neural stem cells of neonatal rat hippocampi / 中华神经医学杂志

Objective To explore whether neural stern cells in vitro express the chemokine receptor CX3CR1. Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in a serum-flee medium. Using immunofluorescence, the neural stem cells and their differentiation into neural and glial cell types were identified; then, the immunofluorescence and RT-PCR were employed to detect whether the neural stem cells in vitro expressed chemokine receptor CX3CR1. Results The floating neural spheres, isolated from neonatal rat hippocampi, were nestin positive and could differentiate into cells to express characteristic antigens of neurons, astrocytes and oligodendrocytes, Immunofluorescence and RT-PCR showed that neural stem cells expressed chemokine receptor CX3CR1. Conclusion Neural stem cells in vitro can express chemokine receptor CX3CR1, which offers the theoretical evidence for further study of its migration.