ABSTRACT
Based on the investigation in Nanji Islands sea area of Zhejiang Province from April 2006 to March 2007, the dynamics of Alexandrium tamarense and its relations with environmental factors were studied. In the study sea area, A. tamarense cells could only be found in spring (from April to June), and the cell abundance was of single peak curve, with the highest (12250 cells x L(-1)) in surface water on 8th of May. Higher cell abundance of A. tamarense was found at the water temperature of 18.5 degrees C - 19.5 degrees C, salinity of 29.5 per thousand - 31.0 per thousand, and lower PO4-P concentration, but no correlation was observed between A. tamarense cell abundance and water dissolved inorganic N concentration. Linear stepwise regression revealed that there was a significant positive correlation between the cell abundance of A. tamarense and the ratio of water dissolved inorganic N to PO4-P.
Subject(s)
Dinoflagellida/growth & development , Nitrogen/analysis , Phosphates/analysis , Seawater/analysis , Animals , China , Dinoflagellida/metabolism , Marine Toxins/analysis , Oceans and Seas , Population DynamicsABSTRACT
We use direct-write laser micromachining technology to fabricate the microfluidic chip, and to establish a microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads, for detecting alpha- fetoprotein (AFP). The AFP analysis can be completed in 20 minutes with 5 microl sample and 5 microl reagent, and there is a good linear correlation in the range of 1-800 ng/ml.
Subject(s)
Humans , Equipment Design , Immunoassay , Methods , Luminescent Measurements , Methods , Microfluidic Analytical Techniques , alpha-FetoproteinsABSTRACT
Objective To investigate the significance of TGF-β1, TGFRl and TGFR2 in the pathogenesis and prognosis in patients with myelofibrosis. Methods The expression of TGF-β1 and its receptors (TGFR1 and TGFR2 ) in bone marrow tissues and the level of TGF-β1 in the blood of 23 patients with myelofibrosis were detected by SABC immunocytochemistry and ELISA repectively. Results Expression of TGF-β1 and TGFR 1 was significantly higher in primary and secondary myelofibrosis patients than that of the control. No significant difference of TGFR2 expression was found between the groups of myelofibrosis and the control (P>0.05). The level of TGF-β1 in the blood of the patients with myelofibrosis was significantly higher than that of the control (P<0.01) and more obvious in secondary cases while TGF-β1 decreased nearly to the normal level when patients were in clinical remission. Conclusion TGF-β1 and it's receptors may be involved in the pathogenesis of myelofibrosis and might be of importance for the prognosis of the patients with myelofibrosis.
ABSTRACT
Objective To investigate the significance of TGF-β1, TGFRl and TGFR2 in the pathogenesis and prognosis in patients with myelofibrosis. Methods The expression of TGF-β1 and its receptors (TGFR1 and TGFR2 ) in bone marrow tissues and the level of TGF-β1 in the blood of 23 patients with myelofibrosis were detected by SABC immunocytochemistry and ELISA repectively. Results Expression of TGF-β1 and TGFR 1 was significantly higher in primary and secondary myelofibrosis patients than that of the control. No significant difference of TGFR2 expression was found between the groups of myelofibrosis and the control (P>0.05). The level of TGF-β1 in the blood of the patients with myelofibrosis was significantly higher than that of the control (P<0.01) and more obvious in secondary cases while TGF-β1 decreased nearly to the normal level when patients were in clinical remission. Conclusion TGF-β1 and it's receptors may be involved in the pathogenesis of myelofibrosis and might be of importance for the prognosis of the patients with myelofibrosis.
