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Objective To analyze the expression levels of matrix metallo proteinase-2 (MMP-2) and matrix metallo proteinase-9 (MMP-9) in serum of patients with esophageal cancer,and to analyze the interrelations among MMP-2,MMP-9 and clinicopathologic characteristics of esophageal cancer.Methods The levels of serum MMP-2,MMP-9 from 60 patients with esophageal cancer were detected by enzyme linked immunosorbent assay.The interrelations among MMP-2,MMP-9 and clinicopathologic characteristics of esophageal cancer including age,gender,histological type,whether or not lymph nodes metastasis and TNM staging were analyzed.Results The levels of MMP-2 and MMP-9 in control group were 61.31 ng/ml and 87.67 ng/ml,the levels of MMP-2 and MMP-9 in patients with esophageal cancer were 121.66 ng/ml and 169.73 ng/ml.The levels of MMP-2 and MMP-9 were significantly higher in patients with esophageal cancer than that of general population (t =3.015,P =0.007; t =2.037,P =0.04).The level of MMP-2 was significantly associated with whether or not lymph node metastasis (t =2.150,P =0.04) and clinical staging (t =2.186,P=0.03).The level of MMP-9 was significantly associated with whether or not lymph node metastasis (t =2.390,P =0.02) and clinical staging (t =2.149,P =0.03).The levels of MMP-2 and MMP-9 have no reference to age,gender and histological type (all P＞0.05).Conclusion MMP-2 and MMP-9 may correlate with tumor invasion and metastasis in patients with esophageal cancer.
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BACKGROUND: The aim of this study was to separate bactericidal proteins from healthy female cervical mucus. METHODS: Cervical mucus was collected and dissolved in 1% acetic acid. The antimicrobial activity of acid-soluble extracts was detected by gel overlay assay against Escherichia coli ATCC 43827. The extracts showed considerable amount of antibacterial activity with a clearly visible band. The bactericidal band was purified by reverse-phase high performance liquid chromatography and the antibacterial activity of the eluate was examined using radial diffusion assay. RESULTS: Two antimicrobial proteins were purified and were further characterized by Tricine sodium dodecyl sulphate-polyacrylamide gel electrophoresis, N-terminal sequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The proteins were identified as high-mobility group nucleosomal-binding domain 2 (HMG N2) and secretory leukocyte peptidase inhibitor (SLPI). SLPI is an antimicrobial peptide already known in the cervical mucus while HMG N2 in the cervical mucus had not been previously reported. The expression of HMG N2 mRNA was detected in Hela cells and cervical epithelial cells by RT-PCR. Slit hybridization showed abundant amounts of the HMG N2 protein in the cervical mucus. CONCLUSIONS: These results suggest that the expression of HMG N2 and SLPI in the healthy female cervical mucus may be relevant to their immune surveillance and defense against potential pathogens in human reproductive system.
Subject(s)
Anti-Infective Agents/chemistry , Cervix Uteri/metabolism , Escherichia coli/metabolism , Mucus/metabolism , Acetic Acid/chemistry , Electrophoresis, Polyacrylamide Gel , Epithelial Cells/metabolism , Female , HeLa Cells , Humans , Immunity , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Secretory Leukocyte Peptidase Inhibitor/isolation & purification , Secretory Leukocyte Peptidase Inhibitor/pharmacology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
To prove the clinical teaching of gynecology of medical foreign students,this paper concludes and analyses the problems of gynecological probation teaching of foreign student through the clinical teaching work,including difficulties in language communication,no unified textbook,particularity of gynecology,and cultural differences.
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Objective To investigate the feasibility of lymphokine activated killer(LAK) cells induced from cord blood used as adoptive cellular immunotherapy for human cancer.Methods Mononuclear cells were separated from umbilical blood(UBMC) by Ficoll,and stimulated by IL-2.The phenotypes(CD3/ CD4/ CD8) of the mononuclear cells were assayed by Flow cytometry,and their lethal activity on K562 or SKOV6 assayed by MTT colometric.The peripheral blood mononuclear cells were used as the control.Results The in vitro anti-tumor effect of LAK from cord blood was significant.Conclusion LAK from cord blood can be a source of adoptive cellular immunotherapy in the treatment of human cancer.
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Objective To study the immune function of gynecologic malignant tumor patients with chemotherapy and the influence of cord blood transfusion.Methods Thirty cases of gynecologic malignant tumor patients with chemotherapy and 30 of healthy women were divided into three groups,MTT colorimetry and flow cytometry was used to determine the change of immune function.Results ①The number of CD4 + T cell in patients was lower than that in healthy women and CD8 + was much higher.The ratio of CD4 +/CD8 + was inverted( t=2.56,P
