ABSTRACT
Objective:To determine the content of baicalin in Zhisou Kechuanning syrup.Methods:.HPLC was used and di erent derivatization reaction conditions were investigated with the optimal conditions found to be the following:the chromatography column was Waters symmetry C 18(5?m;4.6?150mm),the mobile phase(solvent) was a mixture of methanol-0.1% Phosphoric acid(45:55)with a ow rate of 0.8 ml/min,and the detection wavelength was 280nm.Results:The linear range of Bergenin was 0.41-8.13?g with correlation factor r=0.9997,the average recovery was 98.36% and RSD=1.08%.Conclusion:This method is simple,reproducible,and can be used for quantitative determination of baicalin in Zhisou Kechuanning Syrup.
ABSTRACT
OBJECTIVE:To establish an HPLC method for the content determination of pseudoephedrine hydrochloride and dextromethorphan hydrobromide in pediatric pseudoephedrine dextromethorphan drops. METHODS:The separation was performed nomn .A Tghilee nftl oCw18 rcaotelu wmans wseitth a tm 0o.b6i lme Lp?hmasien -o1f amnedt hthaen oiln-j ewcatitoern- vtroieluthmynla mwainse 1(05 ?60L .∶ 4R3E0S ∶ U10L,TSp:H=Th3e. 0l)ineaat rd reatnegctei own ewrea v7e.3le4n～gt7h3 o.4f ?2g0?9 mL-1 for pseudoephedrine hydrochloride(r=0.999 9,n=5)with an average recovery of 99.1%(RSD=0.76%,n=9)and 2.55～25.5 ?g?mL-1 for dextromethorphan hydrobromide(r=0.999 9,n=5)with an average recovery of 98.0%(RSD=0.87%,n=9). CONCLUSION:The method is accurate and reproducible for quality control of pediatric pseudoephedrine dextrometharphan drops.
