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1.
Contrast Media Mol Imaging ; 2022: 2995464, 2022.
Article in English | MEDLINE | ID: mdl-35935306

ABSTRACT

Our aim of this study was to observe and analyze the performance of the real-time fluorescence quantitative nucleic acid amplification detection of Mycobacterium tuberculosis/rifampicin resistance (GeneXpert MTB/RIF), gene chip technology, and modified Roche culture method in detecting MTB in sputum submitted for inspection and drug susceptibility. Patients with smear-negative suspected pulmonary TB (n = 120) in our hospital were enrolled in this study using a random number table, and sputum samples submitted for inspection were tested by the GeneXpert MTB/RIF, gene chip technology, and modified Roche culture method. With clinical diagnosis as the gold standard, the performance (mainly sensitivity and specificity) of the above three detection methods in the diagnosis of MTB was compared. Next, the drug susceptibility test (DST) was carried out on sputum samples, tested positive by the three methods. With the solid culture results as the evaluation criteria, the performance of the three detection methods in the diagnosis MTB and DST was compared. When compared with the modified Roche culture method, the GeneXpert MTB/RIF had the highest positive rate and a shorter overall test duration (P < 0.05). In contrast with the gene chip technology, the GeneXpert MTB/RIF exhibited higher sensitivity and negative predictive value (NPV) and lower specificity, accuracy, positive predictive value (PPV), and Kappa value (P < 0.05). According to analysis of the diagnostic performance of the three detection methods, GeneXpert MTB/RIF displayed the highest diagnostic sensitivity, ideal predictive values, and the highest similarity with clinical diagnosis in results (P < 0.05). The detection of susceptibility to isoniazid (INH) and RIF showed that the GeneXpert MTB/RIF and gene chip technology performed ideally in DST of MTB. In comparison with the modified Roche culture method, the GeneXpert MTB/RIF and gene chip technology have more prominent performance in detecting MTB and drug susceptibility. Besides, to further improve the accuracy of clinical diagnosis, various molecular biology detection methods can be combined to avoid delaying of the best time for the diagnosis and treatment of the disease.


Subject(s)
Mycobacterium tuberculosis , Humans , Mycobacterium tuberculosis/genetics , Oligonucleotide Array Sequence Analysis , Rifampin/pharmacology , Rifampin/therapeutic use , Sensitivity and Specificity , Sputum/microbiology , Technology
2.
J Surg Case Rep ; 2021(2): rjaa581, 2021 Feb.
Article in English | MEDLINE | ID: mdl-33643605

ABSTRACT

Aneurysms in the internal carotid artery, specifically the ophthalmic artery segment, have a lower incidence than any other type of aneurysm. Cases showing simultaneous intracranial aneurysms and meningiomas are extremely rare. This report shares a case of an adult female diagnosed with a deep temporal lobe meningioma concurrent with bilateral internal carotid artery-ophthalmic segment aneurysms. One-stage surgery with coronal incisions and a right frontotemporal craniotomy was performed for this patient. The lesion was first removed along the tumor margin, and the anterior clinoid process was removed. The aneurysm was clipped using an aneurysm clip. The frontal lobe was lifted from the right side, the optic chiasm was separated, the left internal carotid artery was exposed and ophthalmic segment of the left internal carotid artery aneurysm was clipped using a combination of two cross-vessel clips.

3.
Mol Med Rep ; 16(4): 4113-4118, 2017 Oct.
Article in English | MEDLINE | ID: mdl-28765958

ABSTRACT

Interleukin-12 (IL-12) serves an important role in immune responses and antitumor activity. The study of the association between autophagy and cancer cells remains controversial. The present study aimed to investigate the effect of IL­12 on autophagy in the human breast cancer cell lines MDA­MB­231 and MCF­7, and the possible molecular mechanism. Breast cancer cells were treated with different concentrations of recombinant IL­12. The expression of the autophagy-associated protein microtubule­associated protein light chain 3 (LC3) was determined using western blot analysis, fluorescein isothiocyanate­labeled LC3 was detected using fluorescence microscopy and autophagosomes were examined using transmission electron microscopy. Alterations in the phosphatidylinositol 3­kinase/Rac­α serine/threonine protein kinase (PI3K/Akt) and 5'­AMP­activated protein kinase subunit ß­1 (AMPK) pathways, in addition to pathway­associated proteins, were detected using western blotting, following treatment with IL­12 and pretreatment with the PI3K/Akt activator insulin­like growth factor or the AMPK inhibitor compound C. It was observed that IL­12 was able to upregulate the expression of the autophagy­associated protein LC3 in a concentration­ and time­dependent manner, and induce the formation of autophagosomes in the two cell lines, and that the above effects involved the inhibition of the PI3K/Akt signaling pathway and the activation of the AMPK signaling pathway.


Subject(s)
AMP-Activated Protein Kinases/metabolism , Autophagy/drug effects , Breast Neoplasms/enzymology , Breast Neoplasms/pathology , Interleukin-12/pharmacology , Proto-Oncogene Proteins c-akt/metabolism , Signal Transduction/drug effects , Autophagosomes/drug effects , Autophagosomes/metabolism , Autophagosomes/ultrastructure , Breast Neoplasms/ultrastructure , Cell Line, Tumor , Fluorescent Antibody Technique , Humans , Microtubule-Associated Proteins/metabolism
4.
Saudi J Biol Sci ; 24(1): 95-102, 2017 Jan.
Article in English | MEDLINE | ID: mdl-28053577

ABSTRACT

To investigate the effect of over-expression of tissue factor pathway inhibitor-2 (TFPI-2) on the differentiation of hepatocellular carcinoma (HCC) cells (Hep3B and HepG2). The TFPI-2 recombinant adenovirus (pAd-TFPI-2) was constructed using the pAdeasy-1 vector system. Transfected by pAd-TFPI-2, the cell proliferation of HCC cells was evaluated by CCK-8 assay, flow cytometry was used to detect cell apoptosis and CD133 expression. Real-time PCR and Western blot were used to detect the expression levels of markers of hepatocellular cancer stem cells (CSC) and hepatocytes. The over-expression of TFPI-2 significantly suppressed cell proliferation, induced apoptosis, and dramatically decreased the percentage of CD133 cells, which was considered as CSC in HCC. Real-time PCR and Western blot showed that the expression of markers of CSC in Hep3B cells and HepG2 cells infected with pAd-TFPI-2 was markedly lower than those of the control group (P < 0.05), while the expression of markers of hepatocytes was significantly increased (P < 0.05). Hence, TFPI-2 could induce the differentiation of hepatocellular carcinoma cells into hepatocytes, and is expected to serve as a novel way for the treatment of HCC.

5.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 32(7): 865-9, 2016 Jul.
Article in Chinese | MEDLINE | ID: mdl-27363262

ABSTRACT

Objective To investigate the effect of endogenous interferon ß (IFN-ß) on the polarization of M1 macrophages as well as the proliferation and invasion activities of hepatocellular carcinoma cells (HCCs) mediated by M1 macrophages. Methods U937-M1 macrophages derived from human monocytic tumor cells U937 was established and the cell phenotypes were identified by real-time quantitative PCR, ELISA and flow cytometry. After IFN-ß gene was knocked down with siRNA or IFN-ß was neutralized with IFN-ß monoantibody in U937-M1 macrophages, the change of M1/M2 phenotype was again analyzed by the above methods. The expressions of interferon regulatory factor 1 (IRF1) and IRF5 were detected by real-time quantitative PCR and Western blotting. The proliferation and invasion activities of HCCs, which were cultured with conditioned medium (CM) collected from different macrophage groups, were analyzed by CCK-8 assay and Transwell(TM) experiments, respectively. Results U937-M1 macrophages showed higher expressions of interleukin 12p35 (IL-12p35), interleukin 12p40 (IL-12p40), interleukin 12p70 (IL-12p70), interleukin 23p19 (IL-23p19), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and CD86 than U937-M0 did. But both U937-M0 macrophages and U937-M1 macrophages showed low expression of CD206. However, compared with the U937-M1 macrophages, the IFN-ß-blocked U937-M1 macrophages presented decreased expressions of the above M1 macrophages-associated markers, but increased expressions of M2 macrophages-associated markers IL-10 and CD206, as well as lower expressions of IRF1 and IRF5. The inhibited proliferation/invasion activities of HCCs mediated by U937-M1 macrophages were reversed by IFN-ß-blocked U937-M1 macrophages. Conclusion Blocking endogenous IFN-ß could inhibit the U937-M1 polarization status and U937-M1 macrophages-mediated anti-tumor activity of HCCs. IFN-ß might be involved in modulating the expressions of IRF1 and IRF5 as well as maintaining the M1 polarization status and its function.


Subject(s)
Cell Proliferation , Interferon-beta/metabolism , Macrophage Activation , Macrophages/metabolism , Blotting, Western , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Cell Survival/drug effects , Culture Media, Conditioned/pharmacology , Flow Cytometry , Hep G2 Cells , Humans , Interferon Regulatory Factor-1/genetics , Interferon Regulatory Factor-1/metabolism , Interferon Regulatory Factors/genetics , Interferon Regulatory Factors/metabolism , Interferon-beta/genetics , Interleukin-10/genetics , Interleukin-10/metabolism , Interleukin-12/genetics , Interleukin-12/metabolism , Interleukin-23/genetics , Interleukin-23/metabolism , Lectins, C-Type/genetics , Lectins, C-Type/metabolism , Liver Neoplasms/pathology , Macrophages/classification , Mannose Receptor , Mannose-Binding Lectins/genetics , Mannose-Binding Lectins/metabolism , Neoplasm Invasiveness , RNA Interference , Receptors, Cell Surface/genetics , Receptors, Cell Surface/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolism , U937 Cells
6.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 32(7): 870-5, 2016 Jul.
Article in Chinese | MEDLINE | ID: mdl-27363263

ABSTRACT

Objective To investigate the effect of IL-12 on autophagy and the relative possible mechanism in HepG2 and SMMC-7721 human hepatoma cells. Methods The hepatoma cells were treated with IL-12 (10 ng/mL) for 6 hours. Western blotting was applied to detect the expressions of microtubule-associated protein 1 light chain 3 (LC-3), Beclin 1 and the phosphorylated levels of protein kinase B (AKT), mammalian target of rapamycin (mTOR), signal transducer and activator of transcription 3 (STAT3); immunofluorescence assay (IFA) and transmission electron microscopy (TEM) were used to observe the formation of autophagosome. After STAT3 was inhibited by STATTIC or siSTAT3 and AKT was activated by insulin-like growth factor (IGF-1), Western blotting and IFA were performed again to analyze the change of IL-12-induced autophagy. After the cells were treated with IL-12 (10 ng/mL) for 1, 2, 3, 4, 5 days, CCK-8 assay was used to determine the growth ability. After the hepatoma cells were treated with IL-12 (10 ng/mL) for 48 hours, trypan blue staining was used to detect the death rate of the cells. After cell autophagy was inhibit by siBeclin 1, CCK-8 assay and trypan blue staining were performed again to study the effect of IL-12 on the proliferation and death of human hepatoma cells. Results IL-12 induced autophagy and inhibited cell growth in the hepatoma cells. Silencing Beclin 1 gene enhanced IL-12-mediated growth inhibition and cell death. Furthermore, IL-12 treatment also decreased the expressions of p-AKT, p-mTOR and p-STAT3. The pretreatment of siSTAT3 or STATTIC inhibited STAT3-enhanced IL-12-induced autophagy. Accordingly, activation of AKT with IGF-1 decreased IL-12-induced autophagy. Conclusion IL-12 could induce autophagy through AKT/mTOR/STAT3 signaling pathways and the induction of autophagy attenuates the growth-inhibitory effect of IL-12 on hepatoma cells.


Subject(s)
Autophagy/drug effects , Interleukin-12/pharmacology , Proto-Oncogene Proteins c-akt/metabolism , STAT3 Transcription Factor/metabolism , Signal Transduction/drug effects , TOR Serine-Threonine Kinases/metabolism , Autophagosomes/drug effects , Autophagosomes/metabolism , Autophagosomes/ultrastructure , Autophagy/genetics , Blotting, Western , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Cell Proliferation/genetics , Hep G2 Cells , Humans , Liver Neoplasms/genetics , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Microscopy, Electron, Transmission , Microscopy, Fluorescence , Phosphorylation/drug effects , Proto-Oncogene Proteins c-akt/genetics , RNA Interference , STAT3 Transcription Factor/genetics , Signal Transduction/genetics , TOR Serine-Threonine Kinases/genetics , Time Factors
7.
Int J Mol Med ; 38(1): 148-60, 2016 Jul.
Article in English | MEDLINE | ID: mdl-27176664

ABSTRACT

Macrophages that differentiate from precursor monocytes can be polarized into a classically activated (M1) or alternatively activated (M2) status depending on different stimuli. Generally, interferon (IFN)-γ and lipopolysaccharide (LPS) are considered the classical stimuli with which to establish M1 polarization. IFN regulatory factor (IRF)1 and IFN-ß are two crucial molecules involved in IFN-γ- and LPS-initialed signaling. However, the association between IRF1 and IFN-ß in the context of the M1 polarization of macrophages is not yet fully understood. In this study, we demonstrate that U937-derived macrophages, in response to IFN-γ and LPS stimulation, readily acquire an M1 status, indicated by the increased expression of interleukin (IL)-12, IL-6, IL-23, tumor necrosis factor (TNF)-α and the M1-specific cell surface antigen, CD86, and the decreased expression of the M2-specific mannose receptor, CD206. However, the knockdown of IRF1 in U937-derived macrophages led to an impaired M1 status, as indicated by the decreased expression of the above-mentioned M1 markers, and the increased expression of the M2 markers, CD206 and IL-10. A similar phenomenon was observed in the M1 macrophages in which IFN-ß was inhibited. Furthermore, we demonstrated that IRF1 and IFN-ß may interact with each other in the IFN-γ- and LPS-initiated signaling pathway, and contribute to the IRF5 regulation of M1 macrophages. In addition, the conditioned medium collected from the M1 macrophages in which IRF1 or IFN-ß were inhibited, exerted pro-tumor effects on the HepG2 and SMMC-7721 cells, as indicated by an increase in proliferation, the inhibition of apoptosis and an enhanced invasion capability. The findings of our study suggest that the interactions of IRF1, IFN-ß and IRF5 are involved in the M1 polarization of macrophages and have antitumor functions. These data may provide a novel antitumor strategy for targeted cancer therapy.


Subject(s)
Antineoplastic Agents/metabolism , Cell Polarity , Interferon Regulatory Factor-1/metabolism , Interferon-beta/metabolism , Macrophages/cytology , Macrophages/metabolism , Apoptosis/drug effects , Biomarkers/metabolism , Culture Media, Conditioned/pharmacology , Hep G2 Cells , Humans , Interferon Regulatory Factors/metabolism , Interferon-gamma/metabolism , Lipopolysaccharides , Protein Binding/drug effects , U937 Cells , Up-Regulation/drug effects
8.
Mol Cell Biochem ; 415(1-2): 157-68, 2016 Apr.
Article in English | MEDLINE | ID: mdl-27003285

ABSTRACT

Hepatocellular carcinoma is the third most common cause of cancer death worldwide. Novel early detection biomarkers and efficacious therapy strategies are needed. Macrophages recruited from circulation monocytes are the major component of solid cancer and play an important role in the carcinogenesis. Whether overexpression of L-12 in monocytes could induce the phenotype directional differentiation into tumoricidal M1 macrophages and inhibit HCC growth in tumor microenvironment was investigated in this study. For the establishment of the monocyte/IL-12 and polarization of M1-like macrophage, the IL-12 overexpressing recombinant monocyte/IL-12 cells were established by infecting with pAd5F35-CMV/IL-12 adenovirus and co-cultured with HCC SMMC-7721 and Hep3B cells. It was found that the phenotype of monocyte/IL-12 polarized to M1-like macrophages with CD197high IL-12high CD206low IL-10low, and decreased expression of TGF-ß, VEGF-A, and MMP-9. In order to explore the mechanism underlying the macrophages polarization, we detected the Stat-3 pathway and its downstream transcription factor c-myc, and found that the p-Stat-3 and c-myc were down-regulated. To evaluate the effects of monocyte/IL-12 on inhibiting HCC growth, various assays including CCK8, flow cytometry, colony-forming and Transwell assays in vitro, and xenograft mouse models and immunohistochemical analyses in vivo were used to detect the HCC growth and relative markers. Treated with IL-12 overexpressing monocytes, the xenograft tumor growth was significantly inhibited in vivo. These results have proven that IL-12-overexpressed monocytes could directionally differentiate to M1-like macrophages through downregulation of Stat-3 and result in the inhibition of HCC growth.


Subject(s)
Carcinoma, Hepatocellular/pathology , Cell Polarity , Down-Regulation , Interleukin-12/physiology , Liver Neoplasms/pathology , Macrophages/pathology , STAT3 Transcription Factor/physiology , Cell Line, Tumor , Cell Proliferation , Coculture Techniques , Humans , Neoplasm Invasiveness
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