[Biodegradation of di-n-butyl phthalate by Pseudomonas aeruginosa].

A new small capacity-wide extraction method was proposed for detection of its biodegradation in water. Results showed that the halflife (t1/2) of di-n-butyl phthalate (DBP) biodegradation was 3.60 day when the concentration of DBP was 400 mg/L and the biomass concentration was 2 g/L. The biodegradation process conformed to the first-order kinetic model. Moreover, the whole degradation process could be divided into several steps: adsorption, desorption and degradation. Two metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry, which confirmed the dioxygenate process during the hydrolysis of DBP.