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1.
Huan Jing Ke Xue ; 36(11): 4208-17, 2015 Nov.
Article in Chinese | MEDLINE | ID: mdl-26911011

ABSTRACT

This study aimed to analyze the effect of temperature on performance and microbial community structure of an anaerobic packed bed reactor (APBR). The temperature was increased step-wise from room temperature (22 degrees C ± 1 degrees C) to psychrophilic (15 degrees C ± 1 degrees C), mesophilic (37 degrees C ± 1 degrees C) and thermophilic (55 degrees C ± 1 degrees C). The results showed that, in the temperature changing process, the higher the temperature of APBR was, the higher COD removal rate and daily gas production were. After temperature changed to psychrophilic, mesophilic and thermophilic, COD removal rate and daily gas production were 25%, 45%, 60% and 2.3 L x d(-1), 4.0 L x d(-1), 8.5 L x d(-1) respectively. However, there was no significant change in biogas composition (-60%). A sudden temperature change caused a simultaneous increase in the concentration of volatile fatty acids (VFA), which had been fluctuating. Using 16S rRNA gene clone library screening, Euryarchaeota was commonly found, including important methanogens: MBT (Methanobacteriales), Mst (Methanosaetaceae) , Msc (Methanosarcinaceae) and MMB (Methanomicrobiales), as well as thermophilic bacteria and few spring Archaea. However, the diversity of methanogenic groups was reduced, especially at mesophilic. The results of quantitative PCR showed that the 16S rRNA gene concentrations of Mst, MMB and Msc were reduced by temperature changes. Although the relative proportion of every kind of methanogen was significantly affected, Mst was the dominant methanogen.


Subject(s)
Bioreactors/microbiology , Temperature , Archaea , Euryarchaeota , Methanobacteriales , Methanosarcinales , RNA, Ribosomal, 16S
2.
Asian Pac J Cancer Prev ; 16(18): 8637-44, 2015.
Article in English | MEDLINE | ID: mdl-26745130

ABSTRACT

BACKGROUND: Malignant serous effusions (MSE) are one complication in patients with advanced cancer. Endostar is a new anti-tumor drug targeting vessels which exerts potent inhibition of neovascularization. This study aimed to systematically evaluate the efficacy and safety of intraperitoneal perfusion therapy of Endostar combined with platinum chemotherapy for malignant serous effusions (MSE). MATERIALS AND METHODS: Randomized controlled trials (RCTs) on intraperitoneal perfusion therapy of Endostar combined with platinum chemotherapy for malignant serous effusions were searched in the electronic data of PubMed, EMBASE, Web of Science, CNKI, VIP, CBM and WanFang. The quality of RCTs was evaluated by two independent researchers and a meta-analysis was performed using RevMan 5.3 software. RESULTS: The total of 25 RCTs included in the meta-analysis covered 1,253 patients, and all literature quality was evaluated as "B" grade. The meta-analysis showed that Endostar combined with platinum had an advantage over platinum alone in terms of response rate of effusions (76% vs 48%, RR=1.63, 95%CI: 1.50-1.78, P<0.00001) and improvement rate in quality of life (69% vs 44%, RR=1.57, 95%CI: 1.42-1.74, P<0.00001). As for safety, there was no significant difference between the two groups in the incidences of nausea and vomiting (35% vs 34%, RR=1.01, 95%CI: 0.87-1.18, P=0.88), leucopenia (38% vs 38%, RR=1, 95%CI: 0.87-1.15, P=0.99), and renal impairment (18% vs 20%, RR=0.86, 95%CI: 0.43-1.74, P=0.68). CONCLUSIONS: Endostar combined with platinum by intraperitoneal perfusion is effective for malignant serous effusions, and patient quality of life is significantly improved without the incidence of adverse reactions being obviously increased.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Pleural Effusion, Malignant/therapy , Case-Control Studies , Endostatins/administration & dosage , Humans , Infusions, Parenteral , Perfusion , Platinum/administration & dosage , Prognosis , Randomized Controlled Trials as Topic
3.
Eur J Med Chem ; 44(11): 4367-75, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19581024

ABSTRACT

Quantitative structure-activity relationship analysis for recently synthesized aryl alkanol piperazine derivatives was studied for their antidepressant activities. The statistically significant 2D-QSAR models (r(2)>0.924, r(-CV)(2)>0.870, r(-pred)(2)>0.890) were developed using genetic function approximation (GFA) when the number of descriptors in equation was set to four, indicating the descriptors of Atype_C_6, Dipole-mag, S_sssCH and Jurs-PNSA-3 mainly influence the 5-hydroxytryptamine (5-HT) reuptake inhibition activity while the descriptors of HOMO, PMI-mag, S_sssN and Shadow-XZ may chiefly control the noradrenaline (NA) reuptake inhibition activity. The results of the 2D-QSAR models were further compared with 3D-QSAR models generated by molecular field analysis (MFA), investigating the substitutional requirements for the favorable receptor-drug interaction and providing useful information in the characterization and differentiation of their binding sites. The results derived may be useful in further designing novel antidepressants prior to synthesis.


Subject(s)
Antidepressive Agents/chemistry , Antidepressive Agents/pharmacology , Piperazines/chemistry , Piperazines/pharmacology , Antidepressive Agents/chemical synthesis , Binding Sites , Drug Design , Models, Molecular , Molecular Structure , Piperazines/chemical synthesis , Quantitative Structure-Activity Relationship , Serotonin/metabolism
4.
J Integr Plant Biol ; 51(6): 581-92, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19522817

ABSTRACT

Jasmonates and related compounds, including amino acid conjugates of jasmonic acid, have regulatory functions in the signaling pathway for plant developmental processes and responses to the complex equilibrium of biotic and abiotic stress. But the molecular details of the signaling mechanism are still poorly understood. Statistically significant quantitative structure-property relationship models (r(2) > 0.990) constructed by genetic function approximation and molecular field analysis were generated for the purpose of deriving structural requirements for lipophilicity of amino acid conjugates of jasmonic acid. The best models derived in the present study provide some valuable academic information in terms of the 2/3D-descriptors influencing the lipophilicity, which may contribute to further understanding the mechanism of exogenous application of jasmonates in their signaling pathway and designing novel analogs of jasmonic acid as ecological pesticides.


Subject(s)
Amino Acids/chemistry , Cyclopentanes/chemistry , Cyclopentanes/metabolism , Oxylipins/chemistry , Oxylipins/metabolism , Plants/immunology , Quantitative Structure-Activity Relationship , Signal Transduction , Models, Biological
5.
Acta Pharmacol Sin ; 29(11): 1342-9, 2008 Nov.
Article in English | MEDLINE | ID: mdl-18954529

ABSTRACT

AIM: To study the expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats. METHODS: pCMV.Ins, an expression plasmid of the human insulin gene, was constructed. In total, 100 microg pCMV.Ins wrapped with chitosan nanoparticles (chitosan-pCMV.Ins) was transfected to NIH3T3 cells and diabetes rats through lavage and coloclysis, respectively. The transfected cells were grown in Dulbecco's modified Eagle's medium, containing G418, for 72 h after transfection. The clones were selected and continued to grow in G418 medium for 24 d. The expression of human insulin was detected by immunohistochemistry. Human insulin in the culture medium of transfected cells was measured. Fasting blood glucose and plasma human insulin of diabetic rats were measured for 5 d after transfection. RT-PCR and Western blotting were performed to confirm the expression of the human insulin gene in diabetic rats. RESULTS: Approximately 10% of NIH3T3 cells transfected by chitosan-pCMV.Ins expressed human insulin. Human insulin in the culture medium of NIH3T3 cells transfected by chitosan-pCMV.Ins significantly increased compared with that of the control group (P<0.01). Fasting blood glucose levels of the lavage group and the coloclysis group decreased significantly in 5 d (P<0.01) in comparison, while plasma insulin levels were much higher (P<0.01). The human insulin gene mRNA and human insulin were only detected in the lavage and the coloclysis groups. CONCLUSION: The human insulin gene can be transfected and expressed successfully by chitosan- pCMV.Ins in NIH3T3 cells and diabetes rats, which indicates that chitosan is a promising, non-viral vector for gene expression.


Subject(s)
Chitosan/chemistry , Diabetes Mellitus, Experimental/metabolism , Diabetes Mellitus, Experimental/therapy , Gene Transfer Techniques , Genetic Therapy/methods , Insulin/genetics , Nanoparticles/chemistry , Animals , Cytomegalovirus/genetics , Diabetes Mellitus, Type 1/metabolism , Diabetes Mellitus, Type 1/therapy , Genetic Vectors , Humans , Male , Mice , NIH 3T3 Cells , Plasmids/genetics , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction
6.
Bioorg Med Chem Lett ; 18(20): 5381-6, 2008 Oct 15.
Article in English | MEDLINE | ID: mdl-18835162

ABSTRACT

Predictive quantitative structure-activity relationship analysis was developed for a diverse series of recently synthesized 1-aryl-tetrahydroisoquinoline analogs with anti-HIV activities in this study. The conventional 2D-QSAR models were developed by genetic function approximation (GFA) and stepwise multiple linear regression (MLR) with acceptable explanation of 94.9% and 95.5% and good predicted power of 91.7% and 91.7%, respectively. The results of the 2D-QSAR models were further compared with 3D-QSAR model generated by molecular field analysis (MFA), investigating the substitutional requirements for the favorable receptor-drug interaction and quantitatively indicating the important regions of molecules for their activities. The results obtained by combining these methodologies give insights into the key features for designing more potent analogs against HIV.


Subject(s)
Anti-HIV Agents/pharmacology , Chemistry, Pharmaceutical/methods , HIV Infections/drug therapy , HIV Protease Inhibitors/pharmacology , Tetrahydroisoquinolines/chemistry , Tetrahydroisoquinolines/chemical synthesis , Anti-HIV Agents/chemistry , Drug Design , Drug Evaluation, Preclinical , HIV Protease Inhibitors/chemical synthesis , HIV Reverse Transcriptase/antagonists & inhibitors , Humans , Models, Chemical , Models, Molecular , Models, Statistical , Molecular Conformation , Molecular Structure , Quantitative Structure-Activity Relationship
7.
World J Gastroenterol ; 14(7): 1133-6, 2008 Feb 21.
Article in English | MEDLINE | ID: mdl-18286699

ABSTRACT

AIM: To investigate the possibilities and advantages of laparoscopic hepatic left lateral lobectomy combined with fiber choledochoscopic exploration of the common bile duct compaired with traditional open operation. METHODS: Laparoscopic hepatic left lateral lobectomy combined with fiber choledochoscopic exploration of the common bile duct and traditional open operation were performed in two groups of patients who had gallstones in the left lobe of liver and in the common bile duct. The hospitalization time, hospitalization costs, operation time, operative complications and post-operative liver functions of the two groups of patients were studied. RESULTS: The operation time and post-operative liver functions of the two groups of patients had no significant differences, while the hospitalization time, hospitalization costs and operative complications of the laparoscopic hepatic left lateral lobectomy combined with fiber choledochoscopic exploration in the common bile duct group were significantly lower than those in the traditional open operation group. CONCLUSION: For patients with gallstones in the left lobe of liver and in the common bile duct, laparoscopic hepatic left lateral lobectomy combined with fiber choledochoscopic exploration of the common bile duct can significantly shorten the hospitalization time, reduce the hospitalization costs and the post-operative complications, without prolonging the operation time and bringing about more liver function damages compared with traditional open operation. This kind of operation has more advantages than traditional open operation.


Subject(s)
Digestive System Surgical Procedures/methods , Gallstones/surgery , Adult , Cholecystectomy, Laparoscopic/methods , Digestive System Surgical Procedures/adverse effects , Endoscopy, Digestive System/methods , Female , Hepatectomy/methods , Humans , Laparoscopy/methods , Liver Diseases/surgery , Male , Middle Aged , Postoperative Complications/etiology
8.
Huan Jing Ke Xue ; 28(8): 1802-5, 2007 Aug.
Article in Chinese | MEDLINE | ID: mdl-17926414

ABSTRACT

In order to study the distributions characteristics, sources and relationship of PAHs in PM10- phoenix tree leaves-soil system of a coking & chemical factory in Shanghai, the samples of PM10, phoenix tree leaves and soil around the factory were collected for a year. The concentration of PAHs were analyzed according to the USEPA method 8 000 series. The results showed that the average concentration of PAHs in PM10, phoenix tree leaves and soil were 101.11 ng/m3, 79.45 ng/g and 121.53 microg/g, respectively. Particulate phase (PM10) contained mainly carcinogenic and mutagenic PAHs, among which BaA, BghiP, Flu and BaP were found at significant concentrations. In phoenix tree leaves, Nap,Chy, BaP and BghiP presented a higher level of concentration. In soil, 3 and 4-ring PAHs presented a higher level. PAHs concentrations of phoenix tree leaves were very lower in May. Only Ace (0.16 ng/g) and Pyr (0.63 ng/g) were detected. In July and August the concentrations (39.19 ng/g and 150.94 ng/g, respectively) were uplifted significantly. It could be concluded PAHs was from petroleum and coal-fired compound source. There were very strong positive relationships of 16 PAHs level among phoenix tree leaves, soil and PM10 (p < 0.01).


Subject(s)
Air Pollutants/analysis , Particulate Matter/analysis , Polycyclic Aromatic Hydrocarbons/analysis , Soil Pollutants/analysis , Arecaceae/chemistry , Chemical Industry , China , Environmental Monitoring/methods , Industrial Waste , Particle Size , Particulate Matter/chemistry , Plant Leaves/chemistry , Soil/analysis
9.
Bull Environ Contam Toxicol ; 79(4): 448-53, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17712502

ABSTRACT

In order to gain comprehensive understanding of status, properties and sources of PCBs pollution at an industrial area in Shanghai, PM10 were collected during the period November 2004-September 2005. The results showed that the mean value of total PCBs in the industrial area was 2,017.22 pg m(-3). Three dioxin-like PCB congeners had a mean value of TEQ of 0.24 pg-TEQ m(-3). The concentrations of PCBs at all sites were higher in colder months than in warmer months. SigmaPCB concentrations were correlated positively with SO2, NO2 and OCPs, while negatively with polycyclic aromatic hydrocarbons (PAHs), ambient temperature, rainfall and wind speed. It could be concluded that the area had been contaminated by PCBs from a local source.


Subject(s)
Air Pollutants/analysis , Chemical Industry , Environmental Monitoring , Particulate Matter/analysis , Polychlorinated Biphenyls/analysis , Air Pollutants/toxicity , China , Cities , Hydrocarbons, Chlorinated/analysis , Hydrocarbons, Chlorinated/toxicity , Nitrogen Dioxide/analysis , Nitrogen Dioxide/toxicity , Particulate Matter/toxicity , Pesticide Residues/analysis , Pesticide Residues/toxicity , Polychlorinated Biphenyls/toxicity , Polycyclic Aromatic Hydrocarbons/analysis , Polycyclic Aromatic Hydrocarbons/toxicity , Rain , Risk Assessment , Seasons , Sulfur Dioxide/analysis , Sulfur Dioxide/toxicity , Temperature , Wind
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