Bio-enrichment of heavy metals U(VI) in wastewater by protein DSR A.

Uptaking U(VI) from the environment by biological method is an environmental friendly and efficient way. In this work, we have acquired and isolated the protein DSR A by genetic engineering, then assessed its capacity and mechanisms to absorb U(VI) from wastewater. As results, we proved that protein DSR A can precisely recognize, enrich and remove uranyl ions in simulated wastewater solution. Its great removal potential was demonstrated in the adsorption experiments, the adsorption capacity of protein DSR A can reach 182.3 mg/g in the condition at 10 mg/L U(VI) and pH = 6. The Langmuir isotherm model and the pseudo-first-order kinetic equation were used to better describe the absorption process. We can confirm that Na+, Sr2+ and K+, these three metal ions have less effect on the enrichment of U(VI) by protein DSR A compared with other common cations. Besides, we can educe some mechanisms for the removal of U (VI) by protein DSR A from the results of FTIR, SEM-EDS, XPS (binding energy = 2.0 ~ 4.0ke V), MAP and XRD analysis before and after adsorption. This work has demonstrated the great potential of genetic engineering and biological methods in dealing with environmental heavy ion pollution.

Progress of uranium-contaminated soil bioremediation technology.

With the extensive exploitation of nuclear energy and uranium, the problem of uranium-contaminated soil is becoming increasingly prominent. In recent years, various technologies for remediation of uranium-contaminated soil have emerged, such as bioremediation, physical remediation and chemical remediation. Bioremediation technology has the widespread attention because of its environmental friendliness, low cost and high economic benefits. This paper mainly reviews the evaluation index of uranium-contaminated soil, soil remediation technology and its advantages and disadvantages, introduces especially the research status of soil bioremediation technology in detail, and puts forward some suggestions and prospects for bioremediation of uranium-contaminated soil.

Application Progress of Deinococcus radiodurans in Biological Treatment of Radioactive Uranium-Containing Wastewater.

Radioactive uranium wastewater contains a large amount of radionuclide uranium and other heavy metal ions. The radioactive uranium wastewater discharged into the environment will not only pollute the natural environment, but also threat human health. Therefore, the treatment of radioactive uranium wastewater is a current research focus for many researchers. The treatment in radioactive uranium wastewater mainly includes physical, chemical and biological methods. At present, the using of biological treatment to treat uranium in radioactive uranium wastewater has been gradually shown its superiority and advantages. Deinococcus radiodurans is a famous microorganism with the most radiation resistant to ionizing radiation in the world, and can also resist various other extreme pressures. D. radiodurans can be directly used for the adsorption of uranium in radioactive waste water, and it can also transform other functional genes into D. radiodurans to construct genetically engineered bacteria, and then applied to the treatment of radioactive uranium containing wastewater. Radionuclides uranium in radioactive uranium-containing wastewater treated by D. radiodurans involves a lot of mechanisms. This article reviews currently the application of D. radiodurans that directly or construct genetically engineered bacteria in the treatment of radioactive uranium wastewater and discusses the mechanism of D. radiodurans in bioremediation of uranium. The application of constructing an engineered bacteria of D. radiodurans with powerful functions in uranium-containing wastewater is prospected.

Mechanism of recombinant bacteria adsorb UO22+ under culture condition.

Research on the ability and mechanism of genetically recombinant E. coli DH5α containing DSR A gene to enrich uranium under culture conditions provides a theoretical basis for the application of the bacteria in the treatment of uranium pollution. By exploring the influence of factors such as the initial concentration of uranium, culture time, and inoculation amount on the characteristics of uranium enrichment in genetically recombinant E. coli, using FTIR, SEM-EDS, XPS and XRD explore the mechanism of uranium-enriched bacteria. The results showed that when initial UO22+ concentration reach 600 mg/L, E. Coli D1 could not survived, indicated that the maximum tolerance concentration is lower than 600 mg/L. While concentration between 0∼500 mg/L, strains D1 can grow normally and has the ability to enrich uranium. In the prime stage, strains D1 resist toxics through release inorganic phosphates to precipitate UO22+ on cell wall, after 96 h, most UO22+ were transferred into cytoplasm and metabolized into U(IV) which is less toxic. In the metabolize process, all groups involved in metabolizing UO22+, especially protein contain groups like hydroxyl, amine and carboxyl paly a huge role. It shows that within a certain concentration rage, strains D1 has a good enrichment effect on uranium under culture conditions.

[IR and Raman spectroscopic studies on the derivatives of butylphthalide].

dl-NBP is a potentially beneficial and promising drug for treatment of ischemic stroke with multiple actions that affect different pathophysiologic processes, such as improving microcirculation, decreasing brain infarct volume, regulating energy metabolism, and especially inhibiting platelet aggregation and reducing thrombus formation. However, NBP is limited to use in the clinic by other side effects, such as elevated aminotransferase, abnormal liver function and digestive response. Some derivates of NBP were synthesized with the halides (F, Cl and Br) on the 6-position, and their IR and Raman spectra were measured. They proved the complemental information for deducing their structure. By comparing the spectra of the NBP, the band of disubstituted benzene disappeared in the derivatives, and the band of trisubstituted benzenes were observed. The stretching vibrational band of C--H was detected in the Raman spectra, but was not observed in IR. In the low frequency region, the deformation vibration band of --C--C--C--C was also observed in the Raman spectra.

5-Hydr-oxy-1,7-bis-(1H-indol-3-yl)hepta-1,4,6-trien-3-one hemihydrate.

The title compound, C(23)H(18)N(2)O(2)·0.5H(2)O, a derivative of the biologically active compound curcumin, crystallizes with two organic mol-ecules and a solvent water mol-ecule in the asymmetric unit. Each of the two independent mol-ecules is close to being planar (the dihedral angles between the indole ring systems are approximately 9 and 12°) and each exists in the keto-enol form. There is an intra-molecular O-H⋯O hydrogen bond between the keto and enol groups. In the crystal, the components interact by way of N-H⋯N, N-H⋯O and O-H⋯O hydrogen bonds.

[Synthesis and anti-inflammatory activity of hydroxylated E,E-1-(3'-indolyl)-5-(substituted phenyl)-1,4-pentadien-3-one derivatives].

AIM: A series of new 1,4-pentadien-3-one derivatives were synthesized to search for new Eight novel hydroxylated non-steroidal anti-inflammatory drugs (NSAIDs) with potent activity. METHODS: E,E-1-(3'-indolyl)-5-( substituted phenyl)-1,4-pentadien-3-one derivatives were synthesized by means of aldol condensation and characterized by 1H NMR, ESI-MS and element analysis. Their anti-inflammatory activity in vitro were evaluated. RESULTS: Preliminary in vitro pharmacological tests showed that all compounds exhibited anti-inflammatory activity. CONCLUSION: Compounds 4d and 4e exhibited potent anti-inflammatory activity and their anti-inflammatory activity was comparable to resveratrol, and were worthy of further study.

An efficient approach to the asymmetric total synthesis of (-)-anisodine.

-Anisodine (l-6,7-epoxy-3-tropyl-alpha-hydroxytropate), which was isolated from the medicinal plant Scopolia tanguticus Maxim, was the first efficiently prepared using 6-beta-acetyltropine as the starting material via a key step of the Sharpless asymmetric dihydroxylation (AD). The intermediate compounds 10 and 11 showed promising cholinergic activity.