ABSTRACT
Vibrio vulnificus infection caused by contaminated aquatic products and seawater can lead to severe disease and high mortality. The development of a rapid and sensitive detection method for Vibrio vulnificus is vital to effectively prevent infection in advance. In this study, CeO2@PtRu with high peroxidase activity was used to construct a colorimetric immunoassay for Vibrio vulnificus detection by conjugating polyclonal antibodies via the biotin-streptavidin system. The developed colorimetric biosensor for Vibrio vulnificus demonstrated rapid operability and good sensitivity with a detection range from 104 CFU/mL to 109 CFU/mL, and the limit of detection (LOD) is 193 CFU/mL. Moreover, the colorimetric biosensor showed excellent specificity and good recoveries from 98.70% to 102.10% with RSD < 7.45% for spiked real samples. This novel CeO2@PtRu-based colorimetric biosensor has great application potential for the sensitive detection of Vibrio vulnificus in seafood.
Subject(s)
Biosensing Techniques , Cerium , Colorimetry , Seafood , Vibrio vulnificus , Vibrio vulnificus/isolation & purification , Biosensing Techniques/instrumentation , Seafood/microbiology , Seafood/analysis , Cerium/chemistry , Peroxidase/metabolism , Peroxidase/chemistry , Limit of Detection , Food Contamination/analysis , AnimalsABSTRACT
Pillar[5]arene-functionalized rhodium nanoparticles (Rh@CPA NPs) are first synthesized via a facile one-pot chemical reduction method. Rh@CPA NPs with good reusability and biocompatibility show excellent catalytic activities in reducing toxic nitrophenols and azo dyes, and also exhibit superior photothermal ablation capability towards Staphylococcus aureus under 808 nm laser irradiation. This work suggests that the supramolecular capping strategy could be used to construct novel hybrid materials for various applications.