Transcriptional Regulation of Methanol Dehydrogenases in the Methanotrophic Bacterium Methylococcus capsulatus Bath by Soluble and Insoluble Lanthanides.

The effects of soluble and insoluble lanthanides on gene expression in Methylococcus capsulatus Bath were investigated. Genes for lanthanide-containing methanol dehydrogenases (XoxF-MDHs) and their calcium-containing counterparts (MxaFI-MDHs) were up- and down-regulated, respectively, by supplementation with soluble lanthanide chlorides, indicating that M. capsulatus has the "lanthanide switch" observed in other methanotrophs. Insoluble lanthanide oxides also induced the lanthanide switch and were dissolved by the spent medium of M. capsulatus, suggesting the presence of lanthanide-chelating compounds. A transcriptome ana-lysis indicated that a gene cluster for the synthesis of an enterobactin-like metal chelator contributed to the dissolution of insoluble lanthanides.

LncRNA CCHE1 Participates in the Postoperative Distant Recurrence of Urothelial Bladder Cancer Possibly by Regulating ROCK1 Expression.

LncRNA CCHE1 has been functionally characterized as a critical player in multiple cancers. However, its role in urothelial bladder cancer (UBC) is unclear. Therefore, our study aimed to investigate its role in UBC. In our study, we observed that UBC patients who developed distant recurrence (DR) within 5 years after surgical resection showed significantly higher plasma CCHE1 levels than patients with local recurrence (LR) or patients with non-recurrence (NR) on the day of discharge. During the follow-up, plasma CCHE1 levels were significantly increased in UBC patients with DR but slightly decreased in patients with LR and NR. CCHE1 overexpression on the day of discharge distinguished DR patients from LR and NR patients and healthy controls. Moreover, significant and positive correlations between CCHE1 and ROCK1 on the day of discharge and during the follow-up were found across patients with DR. CCHE1 overexpression increased ROCK1 level in UBC cell lines, while ROCK1 overexpression failed to significantly affect CCHE1 expression. Moreover, CCHE1 and ROCK1 overexpression increased UBC cell migration and invasion, and ROCK1 silencing reduced the enhancing effects of CCHE1 overexpression on UBC cell migration and invasion. Therefore, CCHE1 might participate in the postoperative distant recurrence of UBC, possibly by regulating ROCK1 expression.

Identification of Differentially Expressed Genes in Pelvic Organ Prolapse by RNA-Seq.

BACKGROUND Pelvic organ prolapse (POP) brings major health issues for women, affecting 40% of postmenopausal women, and directly affects bladder and bowel function, as well as quality of life. In light of the projected growth in demand for care for pelvic floor disorders, determining the etiology and progression of POP has important public health implications. MATERIAL AND METHODS Uterosacral ligaments (USLs) samples of POP patients and normal controls were enrolled for RNA-Seq, and functional annotation analysis and Protein-Protein interaction (PPI) networks construction were performed for differentially expressed genes (DEGs). RESULTS A total of 81 DEGs were identified between POP and normal control, and distinctly classify all samples into normal and POP group by hierarchical clustering. Sixty-six DEGs demonstrated the same expression pattern among the POP samples with different stages. For those DEGs, canonical Wnt receptor signaling pathway was the most significantly enriched GO term (P value=3.33E-07), and neuroactive ligand-receptor interaction was the most significantly enriched pathway (P value=1.24E-03). In The PPI networks of 81 dysregulated genes, significant hub proteins contained TOP2A (Degree=54), KCNA5 (Degree=22) and PLA2G2A (Degree=19), suggesting their important role in the development of POP. CONCLUSIONS This RNA-seq analysis identified a POP signature of 81 genes, and some ECM-related genes, including COMP, NDP, and SNAI2 might participate in the pathology of POP and be applied as potential therapeutic targets.