ABSTRACT
Thermococcus sp. strain EP1 is a novel anaerobic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent on the East Pacific Rise. It grows optimally at 80 °C and can produce industrial enzymes at high temperature. We report here the draft genome of EP1, which contains 1,819,157 bp with a G+C content of 39.3%. The sequence will provide the genetic basis for better understanding of adaptation to hydrothermal environment and the development of novel thermostable enzymes for industrial application.
Subject(s)
Genome, Archaeal/genetics , Hydrothermal Vents/microbiology , Thermococcus/genetics , Pacific OceanABSTRACT
Here, we report the draft 2,261,881-bp genome sequence of Caloranaerobacter sp. TR13, isolated from a deep-sea hydrothermal vent on the East Pacific Rise. The sequence will be helpful for understanding the genetic and metabolic features, as well as potential biotechnological application in the genus Caloranaerobacter.
ABSTRACT
A taxonomic study was carried out on strain 22II-S11-z7(T), which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase positive, long-rod shaped, and gliding. Growth was observed at salinities of 1-5 % and at temperatures of 10-41 °C. The isolate was capable of hydrolysing gelatin and Tween 80 and able to reduce nitrate to nitrite, but unable to degrade aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z7(T) belongs to the genus Aquimarina, with highest sequence similarity to Aquimarina megaterium XH134(T) (98.31 %), followed by Aquimarina macrocephali JAMB N27(T) (96.59 %); other species of the genus Aquimarina shared 93.63-96.08 % sequence similarity. The ANI value between strain 22II-S11-z7(T) and A. megaterium XH134(T) was found to be 91.86-91.81 %. The DNA-DNA hybridization estimated value between strain 22II-S11-z7(T) and A. megaterium XH134(T) was 47.7 ± 2.6 %. The principal fatty acids were identified as Summed Feature 3 (C16:1 ω7c/ω6c, as defined by the MIDI system; 8.1 %), SummedFeature 9 (iso-C17:1 ω7c/C16:110-methyl; 6.8 %), iso-C15:0 G (11.3 %), iso-C15:0 (24.9 %), iso-C16:0 (5.7 %), C16:0 (5.2 %), iso-C15:0 3OH (6.4 %) and iso-C17:0 3OH (21.5 %). The G+C content of the chromosomal DNA was determined to be 32.99 mol %. The respiratory quinone was determined to be MK-6 (100 %). Phosphatidylethanolamine, two unidentified aminolipids, five unidentified phospholipids and two unidentified lipids were found to be present. The combined genotypic and phenotypic data show that strain 22II-S11-z7(T) represents a novel species within the genus Aquimarina, for which the name Aquimarina atlantica sp. nov. is proposed, with the type strain 22II-S11-z7(T) (=MCCC 1A09239(T) = KCTC 42003(T)).
Subject(s)
Flavobacteriaceae/classification , Flavobacteriaceae/isolation & purification , Seawater/microbiology , Atlantic Ocean , Bacterial Typing Techniques , Base Composition , Cluster Analysis , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Flavobacteriaceae/genetics , Flavobacteriaceae/physiology , Molecular Sequence Data , Phospholipids/analysis , Phylogeny , Quinones/analysis , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sodium Chloride/metabolism , TemperatureABSTRACT
A taxonomic study was carried out on strain 22II-S11-z10(T), which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-stain negative, oxidase and catalase positive, oval- to rod-shaped and non-motile. Growth was observed at salinities of 0.5-9 % and at temperatures of 10-41 °C. The isolate can reduce nitrate to nitrite, degrade gelatin and aesculin, but can not degrade Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z10(T) belongs to the genus Actibacterium, with the highest sequence similarity to the type strain Actibacterium mucosum CECT 7668(T) (97.3 %). The DNA-DNA hybridization estimate value between strain 22II-S11-z10(T) and A. mucosum CECT 7668(T) was 19.30 ± 2.29 %. The principal fatty acids were identified as Summed Feature 8 (C18:1 ω7c/ω6c as defined by the MIDI system, 75.2 %) and Summed Feature 3 (C16:1 ω7c/ω6c, 6.9 %). The G+C content of the chromosomal DNA was determined to be 59.0 mol%. The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylglycerol, phosphatidylcholine, two phospholipids, two aminolipids and two lipids were identified in the polar lipids. The combined genotypic and phenotypic data show that strain 22II-S11-z10(T) represents a novel species within the genus Actibacterium, for which the name Actibacterium atlanticum sp. nov. is proposed, with the type strain 22II-S11-z10(T) (=MCCC 1A09298(T) = LMG 27158(T)).
