ABSTRACT
A new flavonone, named as (2R, 3S)-pinobanksin-3-cinnamate(1), together with six known compounds, pinocem-brin (2), pinobanksin (3), 3-O-acetylpinobanksin (4), galangin (5), kumatakenin(6), and 3-methylkaempferol (7), were isolated from a 95% ethanol extract of seeds of Alpinia katsumadai through a combination of various chromatographic techniques, including silica gel and Sephadex LH-20. The structure of compound 1 was elucidated by spectroscopic data analysis. Compound 1 exhibits a potent neuroprotective effect against the corticosterone-damaged PC12 cells, which may be underlying the effect by scavenging intracellular ROS.
Subject(s)
Alpinia/chemistry , Cholestenones/pharmacology , Cinnamates/pharmacology , Neuroprotective Agents/pharmacology , Seeds/chemistry , Animals , Cell Death/drug effects , Cholestenones/chemistry , Cholestenones/isolation & purification , Cinnamates/chemistry , Cinnamates/isolation & purification , DNA Fragmentation/drug effects , Neuroprotective Agents/chemistry , Neuroprotective Agents/isolation & purification , Oxidative Stress/drug effects , PC12 Cells , Rats , Reactive Oxygen Species/metabolismABSTRACT
Two new compounds, named as (2R,3S)-pinobanksin-3-cinnamate (1), and 15alpha-hydroxy-(22E,24R)-ergosta-3,5,8(14),22-tetraen-7-one (2), were isolated from the endophytic fungus Penicillium sp. FJ-1 of Acanthus ilicifolius Linn. Their structures were elucidated on the basis of spectroscopic analysis. Additionally, compound 1 exhibited potent neuroprotective effects on corticosterone-damaged PC12 cells, and compound 2 showed potent cytotoxicity on glioma cell lines.
