[Resting-state functional magnetic resonance imaging analysis of abnormal directional functional connectivity of the nucleus accumbens in patients with chronic tinnitus].

Objective: To investigate abnormal directional functional connectivity of the nucleus accumbens (NAc) in chronic tinnitus patients using resting-state functional magnetic resonance imaging (fMRI), and to determine the relationship between the degree of this connectivity and tinnitus characteristics. Methods: The resting-state fMRI data of 40 patients with bilateral chronic tinnitus (12 males and 28 females, aged from 26 to 63(50.6±11.6) years) and 40 healthy controls with normal hearing (16 males and 24 females, aged from 26 to 70(45.9±12.4)years) were retrospectively enrolled from the Department of Otolaryngology, Nanjing First Hospital from January 2017 to January 2020. The bilateral NAc were selected as seeds to detect the directional functional connectivity with the whole brain, then the effective connectivity values between the two groups were compared using Granger Causality Analysis (GCA), and the correlation between the effective connectivity and the characteristics of tinnitus was calculated. Results: Compared with healthy controls, the effective connectivity from the left NAc to left middle frontal gyrus in patients with bilateral chronic tinnitus was increased [(1.0±0.2)vs(0.6±0.3)], the effective connectivity from the right NAc to left inferior frontal gyrus was enhanced [(0.9±0.3)vs(0.6±0.4)], the effective connectivity from the right middle temporal gyrus to left NAc was enhanced [(1.0±0.2)vs(0.5±0.3)], the effective connectivity from the right middle frontal gyrus to right NAc was also enhanced[(1.0±0.2)vs(0.5±0.3)](all P<0.05). After adjusting for age, gender, education level, and gray matter volume, positive correlations was observed between the Tinnitus Handicap Questionnaire (THQ) scores and increased effective connectivity values from the left NAc to the left middle frontal gyrus (r=0.386, P=0.020). Additionally, enhanced effective connectivity values from the right middle frontal gyrus to the right NAc was also positively associated with tinnitus duration (r=0.390, P=0.019). Conclusion: The directional functional connectivity between the NAc and prefrontal cortex in patients with chronic tinnitus is enhanced.

Correlations of LTα and NQO1 gene polymorphisms with childhood asthma.

OBJECTIVE: To explore the correlation of the lymphotoxin alpha (LTα) and nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 (NQO1) gene polymorphisms with childhood asthma. PATIENTS AND METHODS: A total of 102 asthma children (observation group) and 80 healthy children (control group) were enrolled. The information was collected via questionnaires and the polymorphisms of LTα rs2844484 and NQO1 rs2917666 were examined with the TaqMan-MGB probe. RESULTS: Observation group had higher constituent ratios of contact with animal furs, personal history of infection, personal history of allergy, familial infection history and familial allergic history than those of control group (p<0.05). However, there were no differences in age, sex, passive smoking, purchase of new furniture and mask wearing between the two groups (p>0.05). The frequency of LTα rs2844484 genotype AA was significantly higher than that of genotype AG and GG (p<0.01), and NQO1 rs2917666 genotype CC showed notably higher frequency than that of genotype CG and GG (p<0.05). The frequency of LTα rs2844484 A allele was significantly higher than that of G allele (p<0.01), while NQO1 rs2917666 C allele had remarkably higher frequency than G allele (p<0.05). The comparisons of the recessive and additive modes revealed differences between the two groups (p<0.05). However, we did not observe significant difference in dominant mode between the two groups (p>0.05). CONCLUSIONS: The risk factors for childhood asthma include the contact with animal furs, personal history of infection, personal history of allergy, familial infection history and familial allergic history. Polymorphisms of LTα and NQO1 genes are correlated with childhood asthma.

Use of Percutaneous Sonographically Guided Microwave Ablation Therapy to Treat Inoperable Malignant Liver Tumours.

AIMS: Microwave ablation (MWA) is a technique which is used to destroy tumours and soft tissues by using microwave energy to create coagulation and localized tissue necrosis. It is used to treat the tumours which are considered to be inoperable and used to treat those patients who are ineligible for surgery due to some factors. The main objective of this study was to evaluate the use of MWA in the treatment of liver cancer. METHODS: The data were collected from Department of Ultrasound, Harbin Medical University Cancer Hospital. From July 2010 to August 2011, a total of 123 patients with liver tumours was referred to Harbin Medical University Cancer Hospital. One hundred patients were selected for this study and treated with MWA. The study group contained 64 (64%) males and 36 (36%) females with an average age (± SD) of 52 (± 5.1) years. RESULTS: One month after therapy, complete ablation was obtained in nodules. The complete ablation rate in tumors ≤ 3 cm and those > 3 cm was 98% and 94%, respectively. Microwave ablation success was higher with nodules ≤ 3 cm (57/58; 98.3%) in comparison to nodules > 3 cm. CONCLUSION: Sonographically guided percutaneous microwave ablation proved to be safe, fast and effective for treatment of hepatocellular carcinoma.

Fouling and cleaning of microfiltration membrane in municipal wastewater reclamation.

This paper presents an investigation on fouling and cleaning of a tubular microfiltration membrane for municipal wastewater reclamation. A bi-level complex model, namely channel clogging and pore plugging, was introduced to elucidate the fouling mechanism. Based on 135 days of microfiltration of activated sludge, a preventive method for channel clogging was reasonably proposed and proven to be effective. Without channel clogging, the operation period was observed a five-fold increase on average, reaching 3-4 weeks. To remove pore plugging, a multi-step chemical cleaning was employed and further optimized in terms of temperature and alkaline concentration. Generally, the chemical cleaning could restore the membrane permeability to higher than 90%.