ABSTRACT
BACKGROUND: Endometrial carcinoma (EC) is the primary cause of death associated with cancer globally. Thus, the possible molecular mechanism of EC needs further exploration. Up-frameshift protein 1 (UPF1) is an ATPase depending on RNA/DNA and RNA helicase depending on ATP. Long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) was dysregulated in diverse diseases. METHODS: qRT-PCR and Western blot were applied to detect UPF1 and PVT1 in EC. CCK-8, colony formation, and Transwell assays were used to test the effects of UPF1/PVT1 on cell proliferation and migration. Cells were cultured with actinomycin D to observe mRNA stability, and RNA immunoprecipitation assay was applied to verified the relationship between UPF1 and PVT1. Glucose consumption and lactate generation were measured when cells were transfected with siRNA. RESULTS: Results demonstrated that the expression of UPF1 exhibited a remarkable decrement in EC tissues relative to that in non-tumor tissues. Subsequent functional experiments suggested that UPF1 decrement stimulated EC cells to grow and migrate. Moreover, UPF1 was discovered to be linked to PVT1 and had an inverse correlation with PVT1. Besides, PVT1 expression affected EC growth and migration, and PVT1 decrement alleviated the influence of UPF1 decrement on EC growth and migration and strengthened glycolysis in EC. CONCLUSION: In this study, we found that UPF1 was down-regulated in EC tissues, and UPF1 might exert its role by regulating the expression of PVT1.
ABSTRACT
AIM: To examine the effects of different temperature protection on measures on preservation damages in liquid blood and explore the corresponding. METHODS: Take equal half blood samples from 10 healthy blood donors and divided each sample into two groups, put the fresh blood into CP2D-A solution at 0 degrees C and 4 degrees C, respectively and take the samples 21 days and 42 days, later and then measured the contents of membrane phospholipids with shafig-UR-rehman method, CaM with purification PED test, LPO with spectrophotometry. RESULTS: At the same temperature, when the preservation time was prolonged, peroxidation was increased, the preservation damages were also augmented; the damages were declined when the temperature was lower during the same period, the aging of blood was more evident at 4 degrees C. CONCLUSION: Blood peroxidation temperature is lower. The author pointed out the questions and prospects of blood preservation.
