ABSTRACT
Two new xanthones, gambogollic acid (1), epigambogollic acid (2), together with three rare compounds, gambogellic acid (3), epigambogellic acid (4) and gambogic acid (5), were isolated from the processed gamboge. The new structures were determined by 1D and 2D NMR spectroscopic analysis. And the cytotoxicity of these five compounds was evaluated against human hepatoma carcinoma and human lung adenocarcinoma cell. Two new compounds showed excellent antitumor activity. All five compounds exhibited inhibitory effect against SMMC-7221cell and A549 cell.
Subject(s)
Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Garcinia/chemistry , Xanthones/chemistry , Xanthones/pharmacology , Cell Line, Tumor , Drug Screening Assays, Antitumor , Humans , Magnetic Resonance Spectroscopy , Spectrophotometry, UltravioletABSTRACT
OBJECTIVE: To study the effect on bioactive components of Xuesaitong injection in the process of ultra-filter by orthogonal test. METHODS: The change of the contents of active components was examined by HPLC method, using notoginsennoside R1 , ginsennoside Rg1, ginsennoside Rb1 and ginsennoside Rd as the mark components. The effect of the transmissivity of active components was studied with four different membrane parameters (such as pore diameter of membrane, operational pressure, the temperature and the concentration of the officinal liquid) by orthogonal test. RESULTS: In the process of ultra-filter the transmissivity of notoginsennoside R, and ginsennoside Rg, had the same characteristics. Both of the average transmissivities of these two components were in the range of 45% to 95%. The concentration of the officinal liquid showed significant influence. The transmissivities of ginsennoside Rb, and ginsennoside Rd in the process of ultra-filiter had the same characteristic. The average transmissivities of these two components were in the range of 2 8%-62% and 1.8%-45%, respectively. The pore diameter of membrane and the temperature showed significant influence. The concentration of the officinal liquid also showed significant influence on the transmissivity of ginsennoside Rd. CONCLUSION: This study provides an external direction for the preparation of Xuesaitong injection.
