ABSTRACT
A new eremophilane norsesquiterpenoid (1), together with a known eremophilane sesquiterpenoid (2), was isolated from the leaves of Ligularia virgaurea. The structure of 1 was elucidated by a combination of spectroscopic analysis (IR, 1D NMR, 2D NMR, and HR-ESI-MS), and its absolute configuration was determined by a single-crystal X-ray diffraction experiment (with copper radiation). The known compound 2 was identified by comparison of its physical and spectral data with those reported in the literature. Compound 1 was assayed for its cytotoxic activities against human cervical carcinoma cell (HeLa) and human small cell lung cancer cell (NCI-446) lines.
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Asteraceae/chemistry , Sesquiterpenes/isolation & purification , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Crystallography, X-Ray , Drug Screening Assays, Antitumor , HeLa Cells , Humans , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Sesquiterpenes/chemistry , Sesquiterpenes/pharmacologyABSTRACT
Genetics is one of the main courses in agricultural and forestry colleges. However, there is large repetition of teaching contents and joining problems between genetics and the relative courses. The negative effects of above problems are discussed in this paper. In order to relieve the conflict between the increase of genetics contents and the decrease of teaching hours in genetics teaching of undergraduates and provide reference for future textbook compilation, some approaches on solving repetition of teaching content and suggestions on joining problems are put forward.
