ABSTRACT
Non-alcoholic fatty liver disease (NAFLD) is usually associated with obesity. However, it is crucial to recognize that NAFLD can also occur in lean individuals, which is frequently overlooked. Without an approved pharmacological therapy for lean NAFLD, we aimed to investigate whether the Ganjianglingzhu (GJLZ) decoction, a representative traditional Chinese medicine (TCM), protects against lean NAFLD and explore the potential mechanism underlying these protective effects. The mouse model of lean NAFLD was established with a methionine-choline-deficient (MCD) diet in male C57BL/6 mice to be compared with the control group fed the methionine-choline-sufficient (MCS) diet. After four weeks, physiological saline, a low dose of GJLZ decoction (GL), or a high dose of GJLZ decoction (GH) was administered daily by gavage to the MCD group; the MCS group was given physiological saline by gavage. Untargeted metabolomics techniques were used to explore further the potential mechanism of the effects of GJLZ on lean NAFLD. Different doses of GJLZ decoction were able to ameliorate steatosis, inflammation, fibrosis, and oxidative stress in the liver; GL performed a better effect on lean NAFLD. In addition, 78 candidate differential metabolites were screened and identified. Combined with metabolite pathway enrichment analysis, GL was capable of regulating the glucose and lipid metabolite pathway in lean NAFLD and regulating the glycerophospholipid metabolism by altering the levels of sn-3-O-(geranylgeranyl)glycerol 1-phosphate and lysoPC(P-18:0/0:0). GJLZ may protect against the development of lean NAFLD by regulating glucose and lipid metabolism, inhibiting the levels of sn-3-O-(geranylgeranyl)glycerol 1-phosphate and lysoPC(P-18:0/0:0) in glycerophospholipid metabolism.
ABSTRACT
As an important basis for the optimization of territorial space, ecological management zoning is of great significance for maintaining regional ecological security and promoting the construction of ecological civilization. With 10 ecosystem services, such as wind break and sand fixation, water conservation, and forest and grass supply, we built a supply index system for Xinjiang. Modelling and ecological economics methods were used to quantify ecosystem service supply. Ecosystem service demand of Xinjiang in 2020 was quantitatively assessed by combining land use intensity, population, and economic status. Based on the ecosystem service supply-demand ratio model and quadrant matching method, we explored the matching relationship and spatial differentiation of ecosystem ser-vice supply and demands on the 1 km grid scale. The breaking point formula and field intensity formula were used to evaluate the flow range and intensity of ecosystem services, and then ecological management zones were divided and corresponding control measures were proposed. The results showed obvious spatial differences in the supply and demand of ecosystem services in Xinjiang in 2020. The high-supply areas were mainly distributed in river valleys and along river systems, while the demand was concentrated in oasis-central cities. The overall supply of ecosystem services was less than the demand. The spatial distribution was dominated by low supply-low demand areas and high supply-high demand areas. There were seven output zones of ecosystem services in Xinjiang, namely Fuyun County, Fuhai County, Yizhou District, Shanshan County, Alashankou City, Keping County and Qira County. The rests were input zones. According to the comprehensive analysis, Xinjiang could be divided into five ecological management areas, i.e., mountain ecological barrier area, oasis ecological restoration area, desert ecological improvement area, desert-oasis ecological protection area, and patch ecological transport area.
