ABSTRACT
Insect cell-baculovirus system is a useful tool for both insecticidal virus production and the expression of medically useful foreign genes. Serum-free culture or low serum culture for insect cells is essential and significant. Media for the growth of insect cells HzAm1 from three kinds of commercial media TC-100,GRACE and IPL-41 with 10% serum were investigated and screened. The result shows that medium TC-100 is the most suitable medium for the growth of cells HzAm1. Adaptation of insect cells HzAm1 to cultures in medium TC-100 with serum reduction from 10% to 1% was carried out as cultures were supplemented with lactalbumin hydrolysate and yeastolate etc. Growth of insect cells HzAml in medium TC-100 with serum 1%, lactalbumin hydrolysate and yeastolate was well.
Subject(s)
Animals , Adaptation, Physiological , Cell Proliferation , Culture Media , Culture Media, Serum-Free , Lepidoptera , Cell BiologyABSTRACT
0.05).The activity of Na~+-K~+ ATPase in SMO group was significantly higher than that in HTK group at 72 h(P