ABSTRACT
Objective To explore the effects of triclosan (TCS) on the immune function of mice. Methods Forty male and female Kunming mice (25±2 g) were selected. The animals were divided into 5 groups according to body weight ratio, including a blank control (saline solution) group, dimethyl sulfoxide (DMSO) group, and three triclosan solution groups (59.375 mg/kg, 118.75 mg/kg, and 237.5 mg/kg, respectively). There were 8 mice in each group, half male and half female. Animals were treated with TCS by intragastric administration once a day for two weeks. Upon the completion of the treatment, animals were sacrificed, the spleen, thymus and other tissues were collected, and the ratios of their weight to body weigh were calculated. The peripheral blood was taken by eye-ball removal method, and the half hemolysis value was determined. Results Compared with the positive control group, the spleen index of male mice in the medium dose group and high dose group increased significantly (P < 0.05), and the spleen index of female mice in the high dose group showed significant difference (P < 0.05). Compared with the positive control group, the thymus index of male high dose group was significantly different (P < 0.05). The thymus indexes of female high, medium, and low dose groups all were significantly different compared to the control group (P < 0.05). HC50 results showed that the HC50 of both male and female mice decreased (P < 0.05). Conclusion High concentration of triclosan can inhibit the immune function of Kunming mice.
ABSTRACT
To review the clinical data of the patients who required repeated renal arteriography following initial super-selective renalartery embolization (SRAE) after Percutaneous Nephrolithotomy (PCNL) and to analyze the reasons for the failure. We retrospectively reviewed the data of the patients who underwent repeat renal arteriography after PCNL for persistent or recurrent severe hemorrhage from August 2001 through August 2015 at our hospital. Total of 427 SRAEs were performed in 369 patients who had undergone PCNL. 21 patients had repeated renal arteriography. Among them, 12 required 2 sessions, 5 required 3 sessions, 1 required 4 sessions, and 3 required 5 sessions. In those 37 repeated renal arteriography, 7 were due to missed pseudoaneurysm, 5 for recanalization of the embolized vessels, and finding of new renal vascular lesions in 22. These new findings included new pseudoaneurysm in sixteen, new arterio-venous fistula (AVF) in two, combination of new pseudoaneurysm and AVF in four, and no identifiable abnormity in three. Except for the missed pseudoaneurysm and the recanalization of the embolized vessels, new renal vascular lesion was the major reason for the repeated arteriography in our hospital. The cause of these new renal vascular lesions was unknown. The lesion might be missed during the initial arteriography due to arterial spasm. Progressive deterioration of the injured vessel integrity could be the other possibility.