ABSTRACT
<p><b>OBJECTIVE</b>To search for natural benzylisoquinoline alkaloids against tumor.</p><p><b>METHOD</b>In this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in vitro.</p><p><b>RESULT</b>Nine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds.</p><p><b>CONCLUSION</b>The developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.</p>
Subject(s)
Humans , Alkaloids , Pharmacology , Antineoplastic Agents, Phytogenic , Pharmacology , Benzylisoquinolines , Pharmacology , Cell Line, Tumor , Cell Proliferation , Chelidonium , Chemistry , Coptis , Chemistry , Drug Evaluation, Preclinical , Drugs, Chinese Herbal , Pharmacology , Neoplasms , Drug Therapy , Papaveraceae , ChemistryABSTRACT
Aim To study the effects and mechanism of Guanxinkang capsules on myocardial injury induced by isoproterenol in rats.Methods 30 male Wistar rats were randomly divided into 3 groups:control group,acute myocardial ischemia group and Guanxinkang capsules group.Rats were given injections of ISO subcutaneously.Electrocardiography were performed,serum and myocardium creatine kinase(CK),creatine kinase MBmass(CK-MB),lactate dehydrogenase(LDH) and nitric oxide(NO) were detected,and the levels of NO and endothelins(ET) in myocardium were measured as well.Results The rising degree of ST-T obviously decreased in Guanxinkang capsules group,and the levels of serum CK,CK-MB and LDH decreased in Guanxinkang capsules group,compared with those of acute myocardial ischemia group(P
