[Exploratory study on influence of biopsy to biological behavior of Tca8113 transplanted tumor].

OBJECTIVE: We established an animal model of nude mice with Tca8113 tumor and cut some tissue for biopsy. We also determined the biological behavior and mechanisms of the tumor. METHODS: The mice were divided into two groups randomly. Mice in both groups were injected with Tca8113 cells into their tongues. The survival condition, growth of primary focus, and metastasis were observed. Hematoxylin and eosin staining and immunohistochemistry were performed on nuclear factor κB (NF-κB), matrix metallopeptidase 9 (MMP-9), vascular endothelial growth factor (VEGF), stromal cell-derived factor 1 (SDF-1), and Ki67 to determine their distributions within the tumor. Cytokeratin staining was also performed to detect micrometastasis in the submandibular lymph nodes. RESULTS: The emerging rate of tumor was 97.92%. The weight and survival time of the experimental group were lower than that of the control group, whereas the metastasis ratio was higher. The expression of NF-κB, MMP-9, SDF-1, and MMP-9 in tumors was higher in the experimental group than that in the control group. The expression of NF-κB, MMP-9, VEGF, and SDF-1 was relevant. The microvessel density of the experimental group was higher than that in the control group. CONCLUSIONS: Biopsy can affect the biological behavior of tongue tumor and can promote growth of primary focus and metastasis.

[Expression and clinical significance of nuclear factor κB/B cell lymphoma-2 signal pathway and glycogen synthase kinase 3ß in oral squamous cell carcinoma].

OBJECTIVE: This study aims to examine the expression of nuclear factor κB (NF-κB)/B cell lymphoma-2 (Bcl-2) signal pathway and glycogen synthase kinase 3ß (GSK-3ß) in oral squamous cell carcinoma (OSCC) and provide references for the early diagnosis and prognosis evaluation of OSCC. METHODS: A total of 55 cases of OSCC and 10 cases of paracan- cerous mucosa were examined in this study. Their expressions of GSK-3ß, NF-κB and Bcl-2 were detected using the SP me- thod immunohistochemistry. The correlation between their expression in OSCC and the clinical and pathological peculiarity of OSCC was analyzed. RESULTS: The positive expression of GSK-3ß, NF-κB, and Bcl-2 in OSCC were significantly higher than that in paracancerous mucosa (P < 0.01). The expression of GSK-3ß, NF-κB, and Bcl-2 had no obvious relationship with patient's age, sex, and clinical stages of the disease (P > 0.05). The expression of Bcl-2 was significantly correlated with the degree of tumor differentiation (P < 0.05), whereas the expression of GSK-3ß and NF-κB in OSCC had no obvious relation- ship with the degree of tumor differentiation (P > 0.05). Strong positive correlations were observed among the expressions of GSK-3ß, NF-κB, and Bcl-2 (P < 0.05). CONCLUSION: The positive expression of GSK-3ß, NF-κB, and Bcl-2 in OSCC are sig- nificantly higher than that in paracancerous mucosa. Detecting GSK-3ß, NF-κB, and Bcl-2 in OSCC may have implications in the early diagnosis and prognosis evaluation of OSCC.

Expression and clinical significance of nuclear factor κB/B cell lymphoma-2 signal pathway and glycogen synthase kinase 3β in oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aims to examine the expression of nuclear factor κB (NF-κB)/B cell lymphoma-2 (Bcl-2) signal pathway and glycogen synthase kinase 3β (GSK-3β) in oral squamous cell carcinoma (OSCC) and provide references for the early diagnosis and prognosis evaluation of OSCC.</p><p><b>METHODS</b>A total of 55 cases of OSCC and 10 cases of paracan- cerous mucosa were examined in this study. Their expressions of GSK-3β, NF-κB and Bcl-2 were detected using the SP me- thod immunohistochemistry. The correlation between their expression in OSCC and the clinical and pathological peculiarity of OSCC was analyzed.</p><p><b>RESULTS</b>The positive expression of GSK-3β, NF-κB, and Bcl-2 in OSCC were significantly higher than that in paracancerous mucosa (P < 0.01). The expression of GSK-3β, NF-κB, and Bcl-2 had no obvious relationship with patient's age, sex, and clinical stages of the disease (P > 0.05). The expression of Bcl-2 was significantly correlated with the degree of tumor differentiation (P < 0.05), whereas the expression of GSK-3β and NF-κB in OSCC had no obvious relation- ship with the degree of tumor differentiation (P > 0.05). Strong positive correlations were observed among the expressions of GSK-3β, NF-κB, and Bcl-2 (P < 0.05).</p><p><b>CONCLUSION</b>The positive expression of GSK-3β, NF-κB, and Bcl-2 in OSCC are sig- nificantly higher than that in paracancerous mucosa. Detecting GSK-3β, NF-κB, and Bcl-2 in OSCC may have implications in the early diagnosis and prognosis evaluation of OSCC.</p>

[Retrospective study of 18 cases with chronic sublinguitis].

OBJECTIVE: To further understand the clinical features of chronic sublinguitis (CS) and to guide the clinical practice by a retrospective study. METHODS: 18 cases with CS were enrolled in the retrospective study and the age, gender, history of present illness, signs, diagnosis and treatment were analyzed. RESULTS: In 18 patients with CS, 15 patients (83.33%) had palpable masses. 8 masses (53.33%) had poor-defined borders, 11 masses (73.33%) were unmovable. There were only 3 patients (16.67%) who were diagnosed correctly in all 18 cases, while 10 patients (55.56%) were diagnosed as tumor. CONCLUSION: CS are often misdiagnosed as sublingual gland tumor and the way to confirm diagnosis is pathological examination. Auxiliary examination should be used properly with clinical manifestation to make the right diagnosis.

Retrospective study of 18 cases with chronic sublinguitis / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To further understand the clinical features of chronic sublinguitis (CS) and to guide the clinical practice by a retrospective study.</p><p><b>METHODS</b>18 cases with CS were enrolled in the retrospective study and the age, gender, history of present illness, signs, diagnosis and treatment were analyzed.</p><p><b>RESULTS</b>In 18 patients with CS, 15 patients (83.33%) had palpable masses. 8 masses (53.33%) had poor-defined borders, 11 masses (73.33%) were unmovable. There were only 3 patients (16.67%) who were diagnosed correctly in all 18 cases, while 10 patients (55.56%) were diagnosed as tumor.</p><p><b>CONCLUSION</b>CS are often misdiagnosed as sublingual gland tumor and the way to confirm diagnosis is pathological examination. Auxiliary examination should be used properly with clinical manifestation to make the right diagnosis.</p>

Effect of mechanical stimulation on the invasion in salivary adenoid cystic carcinoma / 生物医学工程学杂志

This study was aimed to assess the effects of cyclic biaxial mechanical stimulation on the invasion capacity in vitro and on the expression of MMP-9 in the salivary adenoid cystic carcinoma (SACC) high metastatic cell lines ACC-M, and in the SACC low metastatic cell lines ACC-2. We observed the functions of mechanical stimulation in the invasion of cell in vitro and investigated the mechanism involved in the invasion of SACC. Mechanical stimuli were applied to the cells for periods of 1, 3 and 6 hours daily, lasting 2 days. The mechanical stimuli applied to the cells were 4000 micro strain and 1000 micro strain, at a frequency of three hertz. Unstrained cells were used as control. The expressions of MMP-9 on ACC-M and ACC-2 were studied with laser scanning confocal microscope and image analysis software. In vitro invasion capacity of ACC-2 and ACC-M cells was determined by Transwell. The results showed the expression of MMP-9 on the cell of ACC-M was obvious by higher than that on ACC-2. MMP-9 was closely related with the invasion and metastasis of SACC. The mechanical stimulation changed the expression of MMP-9 and the invasion capacity in different duration of mechanical stimulations. Mechanical stimulation can adjust the invasion capacity and expression of MMP-9. It can promote metastasis of the cancer. Moreover, some protein other than MMP-9 may play an important role in the metastasis of the cancer.

Celluar compatibility of the natural human bone derived materials / 实用口腔医学杂志

0.05). ALP activity of the cells on deproteinized bone was higher than that on decalcified bone(P

An experimental study of reinnervation of denevated pectoralis myoflap by hypoglossal-genioglossus muscle neuromuscular pedicle transplantation / 实用口腔医学杂志

砄bjective: To seek for the method of reinnervation of denervated pectoralis major myoflap. Method:The model of pectoralis major myoflap transposition in 20 New Zealand rabbits was used. After the myoflaps were transferred (original nerve innervation,ON) and ON denervation followed by hypoglossal genioglossus muscle neuromuscular pedicle implantation (NMP) wre performed. The flaps were examined at the intervals of 4,8,12 and 16 weeks respectively after operation. Results:In NMP myoflaps contracted when the hypoglosal nerve was stimulated,some type Ⅱ fibers in the myoflaps transformed into type I, but the pattern of distribution of the muscle fibers displayed mosaic and was similar to that of genioglossus muscle. Conclusion:The denervated pectoralis major myoflap can be reinnervated by hypoglossal-genioglossus muscle neuromuscular pedicle transplantation.