ABSTRACT
Objective:To study the relationship between serum soluble intercellular adhesion molecule-1(sICAM-1) and vascular endothelial growth factor(VEGF), placenta growth factor(PLGF), vascular endothelial growth factor receptor 1(VEGFR1, Flt-1), soluble vascular endothelial growth factor receptor 1(sVEGFR1, sFlt-1) mRNA expression in placenta tissue of preeclampsia(PE). Methods: The serum level of sICAM-1 was measured by enzyme-linked immunosorbent assay and gene expression of placenta tissue was detected by quantitative reverse transcriptase-polymerase chain reaction(RT-PCR). Results:(1)The average serum level of sICAM-1 was(218.45±29.93) μg/L in PE group compared with (168.84±19.39) μg/L in controls(P < 0.01).(2)The mRNA expressions of VEGF, PLGF, Flt-1 and sFlt-1 were increased in placenta of PE than those in controls (P < 0.01).(3)There was a positive correlation between the serum level of sICAM-1 and the sFlt-1 mRNA expression in placenta tissue(r = 0.90, P < 0.01). Conclusion: The serum level of sICAM was increased in the patients with PE. The expressions of VEGF, PLGF, Flt-1 and sFlt-1mRNA were increased remarkably in the placenta tissue of palients with PE,especial for sFlt-1. The remarkable increase for expression of sFlt-1mRNA may contribute to endothelial dysfunction, hypertension and proteinuria.
ABSTRACT
Objective To study the protein conformational change of placenta tissue by using Raman spectrum.Methods By using Raman spectroanalysis,we detected the placenta protein conformational change of GDM and control groups.Results(1)In the placenta of GDM,the absorption bands of tryptophan and phenylalanine were increased obviously.(2)In the placenta of GDM,the secondary structure of protein was composed of α-helix,random coil and β-sheet.Conclusions In the placenta of GDM,the orderly conformations of main chains in protein are decreased.Side chains of amino acids,especially tryptophan and phenyl-alanine,are changed greatly.The structure variation of protein may be correlated to the diabetic complications.
ABSTRACT
Serum levels of testosterone (T), free testosterone (T_f), dehydroepiandrosterone sulfate, estradiol and sex hormone binding globulin were measured in patients with pregnancy-induced hypertension syndrome (PIH). Results showed that the levels of T and T_f and mean arterial pressure were significantly higher in the PIH group than those in the control group (all P
