ABSTRACT
COVID-19 patients develop hypolipidemia. However, it is unknown whether lipid levels have improved in recovered patients. In this study, a 3-6 month follow-up study was performed to examine serum levels of laboratory values in 107 discharged COVID-19 patients (mild = 59; severe/critical = 48; diagnoses on admission). 61 patients had a revisit chest CT scan. A Wilcoxon signed-rank test was used to analyze changes in laboratory values. LDL-c and HDL-c levels were significantly higher at follow-up than at admission in severe/critical cases (p < 0.05). LDL-c levels were significantly higher at follow-up than at admission in mild cases (p < 0.05). With adjustment of the factor of traditional Chinese medicine, LDL-c and HDL-c levels were significantly improved at follow-up than at admission in severe/critical cases (p < 0.05). Increases in HDL-c significantly correlated with increases in numbers of white blood cells (p<0.001) and decreases in levels of C-reactive protein (p < 0.05) during patients recovery. Residue lesions were observed in CT images in 69% (42 of 61) of follow-up patients. We concluded that improvements of LDL-c, HDL-c and incomplete absorption of lung lesions were observed at follow-up for recovered patients, indicating that a long-term recovery process could be required.
ABSTRACT
Objective To investigate the role of real-time PCR(qPCR) assay for EBNA fragments in quantitative detection of Epstein-Barr virus(EBV)DNA loads and the diagnose value of combining EBNA assay with common qPCR assay (designed on Bamh1-W fragments) in Nasopharyngeal Carcinoma (NPC) patients Methods EBV DNA loads of 234 blood samples(66 NPC samples included)were detected using two methods and DNA loads inside and outside cells were detected respectively. Positive rate obtained through different methods was compared. Regression analysis and t test were used to validate the methodology. Results Positive rate of EB-NA assay(53.42% in all samples and 51.52% in NPC samples)was lower than that of Bamh1-W assay(69.23%in all samples ,71.21% in NPC samples),however the combination of two methods could enhance the positive rate(70.94% in all samples,72.73% in NPC samples),especially in NPC samples. The correlation R2 of EBNA assay and Bamh1-W assay was 0.577(P < 0.05)and the difference was statistically significant. In NPC samples , R2 was 0.828 (P > 0.05) and it showed good correlation but the difference was not statistically significant. Conclusions The combination of EBNA assay and Bamh1-W assay can improve the positive rate in EBV DNA loads detection and its efficiency is more significant in NPC patients ,which shows significance in EBV DNA loads quantification and in the auxiliary diagnosis of NPC.
ABSTRACT
Objective To investigate the role of real-time PCR(qPCR) assay for EBNA fragments in quantitative detection of Epstein-Barr virus(EBV)DNA loads and the diagnose value of combining EBNA assay with common qPCR assay (designed on Bamh1-W fragments) in Nasopharyngeal Carcinoma (NPC) patients Methods EBV DNA loads of 234 blood samples(66 NPC samples included)were detected using two methods and DNA loads inside and outside cells were detected respectively. Positive rate obtained through different methods was compared. Regression analysis and t test were used to validate the methodology. Results Positive rate of EB-NA assay(53.42% in all samples and 51.52% in NPC samples)was lower than that of Bamh1-W assay(69.23%in all samples ,71.21% in NPC samples),however the combination of two methods could enhance the positive rate(70.94% in all samples,72.73% in NPC samples),especially in NPC samples. The correlation R2 of EBNA assay and Bamh1-W assay was 0.577(P < 0.05)and the difference was statistically significant. In NPC samples , R2 was 0.828 (P > 0.05) and it showed good correlation but the difference was not statistically significant. Conclusions The combination of EBNA assay and Bamh1-W assay can improve the positive rate in EBV DNA loads detection and its efficiency is more significant in NPC patients ,which shows significance in EBV DNA loads quantification and in the auxiliary diagnosis of NPC.
