ABSTRACT
The unsteady foaming-agent mixing ratio in traditional foam-dust-suppression technology limited the dust suppression efficiency. Recent studies proved that the steady mixing ratio could be guaranteed by keeping the jet pumps or Venturis working under cavitation conditions, but the pressure loss of the current devices was over 50%. To decrease the pressure loss under cavitation conditions, we proposed a new mixing device by introducing a spoiler in the Venturi structure. Through computational fluid dynamics (CFD) simulation, the spoiler structure influence on the downstream flow field and the cavitation cloud structure, which affected the total pressure loss of the device, were revealed. For structure optimization, the effect of the other geometric parameters, including the throat length and divergent angle, on the pressure loss was also studied. The proposed device enhanced the cavitation on the suction tube side of the throat; meanwhile, the cavitation in other parts of the device was avoided. Therefore, the cavitation zone in the proposed device was much smaller than that in current devices, and the pressure loss was reduced significantly. When the flow ratio was 0.5-1%, the critical pressure ratio of the proposed mixing device was 0.71-0.68, which indicated that the pressure loss was only 29%-32%. The laboratory experiment verified that when the proposed device worked under cavitation conditions, the accurate and steady mixing ratio was guaranteed. The field experiment indicated that due to the reduced pressure loss of the proposed device, the required water inlet pressure decreased to 0.29 MPa, and the dust suppression rate increased dramatically. This study was of important value in manipulating cavitation cloud structure using a spoiler, clarifying the influence of the cavitation cloud structure on the liquid mixing performance and expanding the application field of the cavitating mixing method.
ABSTRACT
Studies have shown that palmatine (PAL) has anti-cancer effects. However, the activity and potential mechanisms of PAL against colorectal cancer remain elusive. The results showed that PAL significantly inhibited the proliferation of colon cancer cells in vitro and in vivo without significant effect on non-tumorigenic colon cells. Target prediction and clinical sample database analysis suggested that PAL may contribute to colon cancer cells phase arrest and apoptosis by targeting aurora kinase A (AURKA). Inhibition and overexpression of AURKA proved that PAL induces G2/M phase arrest and apoptosis in colon cancer cells by targeting AURKA. Moreover, PAL promoted intracellular Reactive oxygen species (ROS) production and decreased mitochondrial membrane potential (ΔΨm). PAL reduced the levels of AURKA, Bcl-xl and Bcl2 proteins, and promoted the expression of pro-apoptotic proteins P53, P73, Caspase3 and Caspase9, as well as the increase of cytochrome c (cyt. c) in cell lysates in vitro and in vivo. Together, our study confirmed that PAL induced G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA. PAL may provide a novel solution for the treatment of colon cancer by serving as a new AURKA inhibitor.
Subject(s)
Aurora Kinase A/antagonists & inhibitors , Berberine Alkaloids/administration & dosage , Colonic Neoplasms , G2 Phase Cell Cycle Checkpoints/drug effects , M Phase Cell Cycle Checkpoints/drug effects , Mitochondria/drug effects , Animals , Apoptosis/drug effects , Apoptosis/physiology , Aurora Kinase A/metabolism , Colonic Neoplasms/drug therapy , Colonic Neoplasms/metabolism , Dose-Response Relationship, Drug , Drug Delivery Systems/methods , G2 Phase Cell Cycle Checkpoints/physiology , HCT116 Cells , HT29 Cells , Humans , M Phase Cell Cycle Checkpoints/physiology , Mice , Mice, Inbred BALB C , Mice, Nude , Mitochondria/metabolism , Signal Transduction/drug effects , Signal Transduction/physiology , Xenograft Model Antitumor Assays/methodsABSTRACT
Aqueous foam is widely used in fire extinguishing and dust suppression technologies. Improving the foaming ability is the key to reducing the added concentration of foaming agents as well as the economic cost. In this work, the effect of a water-soluble polymer (polyvinyl alcohol, PVA) on the foaming ability of anionic surfactant (sodium dodecyl ether sulfate, SDES) was studied by an experiment and molecular dynamics simulation. The experimental results showed that PVA greatly improves the foaming ability of SDES solutions when the surfactant concentration is less than 0.1%, which is attributed to the fact that the polymer can enhance the stability of bubble films and reduce the bubble rupture rate during the foam generation process. The simulation results indicate that PVA can enhance the hydration of surfactant head groups and contribute to the formation of a three-dimensional hydrogen bond network between surfactants, polymers, and water molecules, thus greatly improving the stability of bubble liquid films. The above results suggest that water-soluble polymers can be used to improve the foaming ability of surfactant solutions by enhancing the bubble film stability, which is beneficial as it reduces the added concentration of foaming agents in aqueous foam applications.
