Multiomic analyses of two sorghum cultivars reveals the change of membrane lipids in their responses to water deficit.

Drought is one of the main abiotic stresses influencing crop production all over the world. Membranes are sensitive to drought stress and easy to be degraded and modified. Lipidome and transcriptome analyses were applied to analyze the responses of membrane lipids to drought stress in two sorghum (Sorghum bicolor (L.) Moench) cultivars, drought-sensitive cv. Hongyingzi and drought-tolerant cv. Kangsi. In total, 156 lipid compounds were identified and the contents of the predominant ones changed significantly under drought stress. Drought significantly decreased the unsaturation indices (UI) of digalactosyl-diacylglycerol (DGDG), monogalactosyl-diacylglycerol (MGDG), phosphatidylglycerol (PG) and phosphatidylcholine (PC) in both cultivars, except for insignificant changes of UI for DGDG in cv. Kangsi. Transcriptome sequencing analysis identified genes related to membrane lipid remodeling such as phospholipase D α1 (PLDα1), phospholipase D δ (PLDδ), and phospholipase A 2 (PLA2). By integrating transcriptome data and lipidome data, weighted gene co-expression network analysis (WGCNA) identified hub genes, transcription factors and the genes involved in lipid metabolism. Then, the protein and protein interaction (PPI) was analyzed using STRING and the possible candidate genes regulating membrane lipids under drought stress were obtained, including CCT2, CER1, DGK1, DGK5, EMB3174, KCS4, LCB2, PAH1, PLDP1, PKP-ß1, and KCS11. The results from this study have the potential to accelerate the process to breed drought-tolerant sorghum lines.

Integrative analysis of the cuticular lipidome and transcriptome of Sorghum bicolor reveals cultivar differences in drought tolerance.

Cuticular wax and cutin are directly involved in the mechanisms by which plants acclimate to water-limited environments. However, how the two lipid forms balance their contributions to plant drought-tolerance is still not clear. The present study examined the responses of cutin monomers and cuticular waxes to drought stress in two sorghum (Sorghum bicolor (L.) Moench) cultivars, drought-tolerant cv. Kangsi and drought-sensitive cv. Hongyingzi, by combining lipidomic and transcriptomic analysis. Drought increased total cutin contents by 41.3%, the contents of alkanoic acids by 72.6% and 2-hydroxyacids by 117.8% in Kangsi but unchanged those in Hongyingzi. The abundance of cutin monomers were relatively stable for cv Hongyingzi, excepting for a decrease of ω-hydroxyacids from 35.0% to 27.4% in drought-stressed plants. However, for cv Kangsi, the abundance of ω-hydroxyacids decreased from 36.8% to 21.0% and that of alkanoic acids increased from 30.5% to 37.1% in drought-stressed plants. Drought increased total wax coverage in Hongyingzi but reduced it in Kangsi. However, the abundance of aldehydes decreased from 51.2% to 39.3% in drought-stressed cv Kangsi, but increased from 25.2% to 36.1% in drought-stressed cv Hongyingzi. A decrease of sterols (by 76%) and an increase of primary alcohol (by 443%) was also observed in drought-stressed cv Hongyingzi. Transcriptome analysis also revealed that many genes implicated by homology in cutin monomer and cuticular wax biosynthesis also differed in their responses to drought stress between the two sorghum cultivars. Therefore, sorghum cultivars differed in their mechanisms in adjusting chemical profiles of both cutin and cuticular wax under water deficit condition.

Chemical profiles of cuticular waxes on various organs of Sorghum bicolor and their antifungal activities.

Sorghum bicolor is widely cultivated in arid and semi-arid areas. This paper reports the chemical profiles of cuticular waxes on adaxial and abaxial sides of common leaf, flag leaf, sheath and stem from six sorghum cultivars and the variations of leaf cuticular waxes at seedling, jointing and filling stages. Then, the bioassay of leaf and sheath wax were evaluated against Penicillium sp and Alternaria alternata. The six sorghum cultivars had similar wax profiles. In total, eight wax compounds were identified, including fatty acids, aldehydes, primary alcohols, alkanes, secondary alcohols, ketones, sterols and minor triterpenoids. Leaf wax coverage increased from 2.2 to 3.1 µg/cm2 at seedling stages to 6.5-14.0 µg/cm2 at jointing and filling stages, respectively. The relative abundance of primary alcohols decreased from 51 to 62% at seedling stage to 17-33% at jointing stage whereas alkanes increased from 5-9% to 19-33%. Leaf was dominated with alkanes (28.4%) and aldehydes (28.4%), sheath with acids (42.8%), and stem with aldehydes (80.8%). Epicuticular wax of leaf and sheath contained higher proportions of alkanes whereas the intracuticular waxes contained higher proportions of sterols. The leaf wax improved the growth of Penicillium but reduced that of A. alternaria, whereas sheath wax reduced the growth of Penicillium but unchanged A. alternaria. The detailed sorghum wax profiles improve our understanding of the physiological roles of these waxes and their diversified potential usages in industries.

Genome-Wide Identification and Structural Analysis of bZIP Transcription Factor Genes in Brassica napus.

The basic region/leucine zipper motif (bZIP) transcription factor family is one of the largest families of transcriptional regulators in plants. bZIP genes have been systematically characterized in some plants, but not in rapeseed (Brassica napus). In this study, we identified 247 BnbZIP genes in the rapeseed genome, which we classified into 10 subfamilies based on phylogenetic analysis of their deduced protein sequences. The BnbZIP genes were grouped into functional clades with Arabidopsis genes with similar putative functions, indicating functional conservation. Genome mapping analysis revealed that the BnbZIPs are distributed unevenly across all 19 chromosomes, and that some of these genes arose through whole-genome duplication and dispersed duplication events. All expression profiles of 247 bZIP genes were extracted from RNA-sequencing data obtained from 17 different B. napus ZS11 tissues with 42 various developmental stages. These genes exhibited different expression patterns in various tissues, revealing that these genes are differentially regulated. Our results provide a valuable foundation for functional dissection of the different BnbZIP homologs in B. napus and its parental lines and for molecular breeding studies of bZIP genes in B. napus.