miR-26a improves microglial activation and neuronal apoptosis in a rat model of cerebral infarction by regulating the TREM1-TLR4/MyD88/NF-κB axis.

Emerging studies have indicated that abnormally expressed microRNAs (miRNAs) are related to the pathogenesis of cerebral ischemia. Nevertheless, the function of miR-26a in neuronal damage and microglial activation during cerebral infarction remains elusive. It was revealed that miR-26a was downregulated in oxygen-glucose deprivation (OGD)-treated microglia and neurons. Overexpressing miR-26a reduced the inflammatory reaction in BV2 cells and decreased neuronal apoptosis following OGD stimulation. miR-26a upregulation inactivated the TLR4/MyD88/NF-κB pathway and inhibited TREM1 expression. Repressing NF-κB phosphorylation inhibited the miR-26a level. As supported by the dual-luciferase reporter assay, TREM1 was directly targeted by miR-26a. Furthermore, a rat model of middle cerebral artery occlusion (MCAO) was built. We discovered that miR-26a improved cognitive, learning and motor functions and reduced cerebral edema in MCAO rats. Mechanistically, upregulating miR-26a reduced inflammation and neuronal apoptosis by mitigating the TREM1-TLR4/MyD88/NF-κB pathway in the MCAO rat model. Collectively, this study verified that the miR-26a-TREM1-TLR4/MyD88/NF-κB axis contributes to modulating OGD-mediated microglial activation and neuronal injury.

Large viewing angle three-dimensional display with smooth motion parallax and accurate depth cues.

A three-dimensional (3D) display with smooth motion parallax and large viewing angle is demonstrated, which is based on a microlens array and a coded two-dimensional (2D) image on a 50 inch liquid crystal device (LCD) panel with the resolution of 3840 × 2160. Combining with accurate depth cues expressing, the flipping images of the traditional integral imaging (II) are eliminated, and smooth motion parallax can be achieved. The image on the LCD panel is coded as an elemental image packed repeatedly, and the depth cue is determined by the repeated period of elemental image. To construct the 3D image with complex depth structure, the varying period of elemental image is required. Here, the detailed principle and coding method are presented. The shape and the texture of a target 3D image are designed by a structure image and an elemental image, respectively. In the experiment, two groups of structure images and their corresponding elemental images are utilized to construct a 3D scene with a football in a green net. The constructed 3D image exhibits obviously enhanced 3D perception and smooth motion parallax. The viewing angle is 60°, which is much larger than that of the traditional II.

Time-reversed ultrasonically encoded optical focusing using two ultrasonic transducers for improved ultrasonic axial resolution.

Focusing light inside highly scattering media is a challenging task in biomedical optical imaging, manipulation, and therapy. A recent invention has overcome this challenge by time reversing ultrasonically encoded diffuse light to an ultrasound-modulated volume inside a turbid medium. In this technique, a photorefractive (PR) crystal or polymer can be used as the phase conjugate mirror for optical time reversal. Accordingly, a relatively long ultrasound burst, whose duration matches the PR response time of the PR material, is usually used to encode the diffuse light. This long burst results in poor focusing resolution along the acoustic axis. In this work, we propose to use two intersecting ultrasound beams, emitted from two ultrasonic transducers at different frequencies, to modulate the diffuse light at the beat frequency within the intersection volume. We show that the time reversal of the light encoded at the beat frequency can converge back to the intersection volume. Experimentally, an acoustic axial resolution of ~1.1 mm was demonstrated inside turbid media, agreeing with theoretical estimation.

The modulation function and realizing method of holographic functional screen.

The modulation function of holographic functional screen (HFS) in the real-time, large-size full-color (RLF), three-dimensional (3D) display system is derived from angular spectrum analysis. The directional laser speckle (DLS) method to realize the HFS is proposed. A HFS by the DLS method was fabricated and used in the experiment. Experimental results show that the HFS is valid in the RLF 3D display, and that the derived modulation function is valuable for the design of the HFS. The research results are important to realize the RLF 3D display system which will find many applications such as holographic video.

Demonstration of a large-size real-time full-color three-dimensional display.

A large-size and full-color three-dimensional (3D) display system without the need for special eyeglasses is demonstrated. With a specially fabricated holographic functional screen with a size of 1.8x1.3 m(2), the system including optimally designed camera-projector arrays and a video server can display the fully continuous, natural 3D scene with more than 1 m image depth in real time. We explain the operating principle and present experimental results.