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1.
Nat Commun ; 13(1): 4428, 2022 07 30.
Article in English | MEDLINE | ID: mdl-35908039

ABSTRACT

Cholesterol sulfate, produced by hydroxysteroid sulfotransferase 2B1 (SULT2B1), is highly abundant in the intestine. Herein, we study the functional role and underlying intestinal epithelial repair mechanisms of cholesterol sulfate in ulcerative colitis. The levels of cholesterol and cholesterol sulfate, as well as the expression of Sult2b1 and genes involved in cholesterol biosynthesis, are significantly higher in inflamed tissues from patients with ulcerative colitis than in intestinal mucosa from healthy controls. Cholesterol sulfate in the gut and circulation is mainly catalyzed by intestinal epithelial SULT2B1. Specific deletion of the Sult2b1 gene in the intestinal epithelial cells aggravates dextran sulfate sodium-induced colitis; however, dietary supplementation with cholesterol sulfate ameliorates this effect in acute and chronic ulcerative colitis in mice. Cholesterol sulfate promotes cholesterol biosynthesis by binding to Niemann-Pick type C2 protein and activating sterol regulatory element binding protein 2 in colonic epithelial cells, thereby alleviates ulcerative colitis. In conclusion, cholesterol sulfate contributes to the healing of the mucosal barrier and exhibits therapeutic efficacy against ulcerative colitis in mice.


Subject(s)
Colitis, Ulcerative , Colitis , Animals , Cholesterol Esters/metabolism , Colitis/metabolism , Colitis, Ulcerative/genetics , Colon/metabolism , Dextran Sulfate/toxicity , Disease Models, Animal , Epithelial Cells/metabolism , Intestinal Mucosa/metabolism , Mice , Mice, Inbred C57BL
2.
Cell Biol Toxicol ; 38(3): 505-530, 2022 06.
Article in English | MEDLINE | ID: mdl-34401974

ABSTRACT

Acetaminophen (APAP) overdose is a common cause of drug-induced liver injury (DILI). Ferroptosis has been recently implicated in APAP-induced liver injury (AILI). However, the functional role and underlying mechanisms of mitochondria in APAP-induced ferroptosis are unclear. In this study, the voltage-dependent anion channel (VDAC) oligomerization inhibitor VBIT-12 and ferroptosis inhibitors were injected via tail vein in APAP-injured mice. Targeted metabolomics and untargeted lipidomic analyses were utilized to explore underlying mechanisms of APAP-induced mitochondrial dysfunction and subsequent ferroptosis. As a result, APAP overdose led to characteristic changes generally observed in ferroptosis. The use of ferroptosis inhibitor ferrostatin-1 (or UAMC3203) and iron chelator deferoxamine further confirmed that ferroptosis was responsible for AILI. Mitochondrial dysfunction, which is associated with the tricarboxylic acid cycle and fatty acid ß-oxidation suppression, may drive APAP-induced ferroptosis in hepatocytes. APAP overdose induced VDAC1 oligomerization in hepatocytes, and protecting mitochondria via VBIT-12 alleviated APAP-induced ferroptosis. Ceramide and cardiolipin levels were increased via UAMC3203 or VBIT-12 in APAP-induced ferroptosis in hepatocytes. Knockdown of Smpd1 and Taz expression responsible for ceramide and cardiolipin synthesis, respectively, aggravated APAP-induced mitochondrial dysfunction and ferroptosis in hepatocytes, whereas Taz overexpression protected against these processes. By immunohistochemical staining, we found that levels of 4-hydroxynonenal (4-HNE) protein adducts were increased in the liver biopsy samples of patients with DILI compared to that in those of patients with autoimmune liver disease, chronic viral hepatitis B, and non-alcoholic fatty liver disease (NAFLD). In summary, protecting mitochondria via inhibiting VDAC1 oligomerization attenuated hepatocyte ferroptosis by restoring ceramide and cardiolipin content in AILI.


Subject(s)
Analgesics, Non-Narcotic , Chemical and Drug Induced Liver Injury , Ferroptosis , Voltage-Dependent Anion Channel 1/metabolism , Acetaminophen/adverse effects , Analgesics, Non-Narcotic/metabolism , Animals , Cardiolipins/metabolism , Ceramides/metabolism , Chemical and Drug Induced Liver Injury/metabolism , Hepatocytes/metabolism , Humans , Liver/pathology , Mice , Mice, Inbred C57BL , Mitochondria/metabolism
3.
J Nutr Biochem ; 97: 108803, 2021 11.
Article in English | MEDLINE | ID: mdl-34147602

ABSTRACT

With its unique cellular plasticity, the small intestinal mucosa exhibits efficient adaptability upon feeding. However, little is known about the effect of high-fat diet (HFD) feeding on this adaption and its underlying mechanism. Herein, we demonstrated that the cell proliferation ability, mitochondrial morphology, and global transcriptomic profile of the small intestine exhibited a prominent discrepancy between the fasted and refed state in mice, which were markedly attenuated by long-term HFD feeding. The retinol (Vitamin A, VA) metabolism pathway was dramatically affected by HFD feeding in the small intestine. Both VA and its active metabolite retinoic acid (RA), with the administration of lipid micelles, promoted the expression of genes involved in lipid absorption and suppressed the expression of genes involved in the cell proliferation of intestinal organoids. Via chip-qPCR and RT-qPCR, genes involved in lipid metabolism and cell proliferation were target genes of RARα/RXRα in small intestinal organoids treated with RA and lipid micelles. The role of VA in the in vivo attenuation of intestinal adaptability, in response to HFD, was evaluated. Mice were fed a normal chow diet, HFD, or HFD diet supplemented with additional 1.5-fold VA for 12 weeks. VA supplementation in HFD accelerated the attenuation of intestinal adaptability upon feeding induced by HFD, promoted lipid absorption gene expression, and increased body weight and serum cholesterol levels. In conclusion, the discrepancy of the small intestine between the fasted and refed state was dramatically attenuated by HFD feeding, in which VA and RA might play important roles.


Subject(s)
Adaptation, Physiological , Diet, High-Fat , Dietary Supplements , Intestinal Mucosa/physiology , Intestine, Small/physiology , Tretinoin/pharmacology , Vitamin A/administration & dosage , Animals , Cell Proliferation/genetics , Eating , Gastrointestinal Microbiome , Intestine, Small/metabolism , Lipid Metabolism/genetics , Lipids/blood , Mice , Mice, Inbred C57BL , Organoids/growth & development , Transcriptome , Tretinoin/metabolism , Vitamin A/metabolism , Vitamin A/pharmacology , Vitamins/administration & dosage , Vitamins/metabolism , Vitamins/pharmacology
4.
Cell Death Dis ; 10(12): 873, 2019 11 18.
Article in English | MEDLINE | ID: mdl-31740661

ABSTRACT

Esophageal squamous cell carcinoma (ESCC) remains one of the most common malignancies in China and has a high metastasis rate and poor prognosis. Cancer-associated fibroblasts (CAFs), a prominent component of the tumor microenvironment, can affect tumor progression and metastasis, but the underlying mechanism remains unclear. There are no studies that explore the role of hydrogen peroxide-inducible clone 5 (HIC-5) in ESCC or compare the role of HIC-5 in CAFs and adjacent noncancerous normal fibroblasts (NFs). In this study, we isolated primary CAFs and NFs from ESCC patients. HIC-5 was highly expressed in CAFs from the tumor stroma of human ESCC patients. HIC-5 knockdown in CAFs inhibited the migration and invasion of ESCC cells in vitro. Supernatant CCL2 levels of CAFs were significantly higher after TGF-ß stimulation and lower after knocking down HIC-5 expression, independent of TGF-ß treatment. HIC-5 knockdown in CAFs led xenograft tumors derived from ESCC cells mixed with CAFs to present more regular morphology, express higher CDH1, and lower CCL2. Further RNA-seq data showed that HIC-5 has distinct biological functions in CAFs vs. NFs, especially in cell movement and the Rho GTPase signaling kinase pathway, which was verified by wound-healing assays and western blotting. An ESCC tissue microarray revealed that increased HIC-5 expression in the tumor stroma was associated with positive lymph node metastasis and a higher TNM stage. In summary, we identified that stromal HIC-5 was a predictive risk factor for lymph node metastasis in human ESCC and that CAF-derived HIC-5 regulated ESCC cell migration and invasion by regulating cytokines and modifying the ECM.


Subject(s)
Cancer-Associated Fibroblasts/metabolism , Esophageal Neoplasms/metabolism , Esophageal Squamous Cell Carcinoma/metabolism , Intracellular Signaling Peptides and Proteins/metabolism , LIM Domain Proteins/metabolism , Animals , Cancer-Associated Fibroblasts/pathology , Cell Line, Tumor , Cell Movement/physiology , Cytokines/metabolism , Disease Progression , Esophageal Neoplasms/pathology , Esophageal Squamous Cell Carcinoma/pathology , Female , Heterografts , Humans , Male , Mice , Mice, Inbred BALB C , Mice, Nude , Middle Aged , Tumor Microenvironment
5.
Lipids Health Dis ; 18(1): 203, 2019 Nov 22.
Article in English | MEDLINE | ID: mdl-31757214

ABSTRACT

BACKGROUND: A healthy gastric mucosal epithelium exhibits tumor-suppressive properties. Gastric epithelial cell dysfunction contributes to gastric cancer development. Oxysterols provided from food or cholesterol oxidation in the gastric epithelium may be further sulfated by hydroxysteroid sulfotransferase 2B1 (SULT2B1), which is highly abundant in the gastric epithelium. However, the effects of SULT2B1 on gastric epithelial function and gastric carcinogenesis are unclear. METHODS: A mouse gastric tumor model was established using carcinogenic agent 3-methylcholanthrene (3-MCA). A SULT2B1 deletion (SULT2B1-/-) human gastric epithelial line GES-1 was constructed by CRISPR/CAS9 genome editing system. RESULTS: The gastric tumor incidence was higher in the SULT2B1-/- mice than in the wild-type (WT) mice. In gastric epithelial cells, adenovirus-mediated SULT2B1b overexpression reduced the levels of oxysterols, such as 24(R/S),25-epoxycholesterol (24(R/S),25-EC) and 27-hydroxycholesterol (27HC). This condition also increased PI3K/AKT signaling to promote gastric epithelial cell proliferation, epithelization, and epithelial development. However, SULT2B1 deletion or SULT2B1 knockdown suppressed PI3K/AKT signaling, epithelial cell epithelization, and wound healing and induced gastric epithelial cell malignant transition upon 3-MCA induction. CONCLUSIONS: The abundant SULT2B1 expression in normal gastric epithelium might maintain epithelial function via the PI3K/AKT signaling pathway and suppress gastric carcinogenesis induced by a carcinogenic agent.


Subject(s)
Carcinogenesis/genetics , Gene Expression Regulation, Neoplastic , Phosphatidylinositol 3-Kinases/genetics , Proto-Oncogene Proteins c-akt/genetics , Stomach Neoplasms/genetics , Sulfotransferases/genetics , Animals , Base Sequence , CRISPR-Cas Systems , Carcinogenesis/drug effects , Carcinogenesis/metabolism , Carcinogenesis/pathology , Cell Line, Tumor , Cell Proliferation , Cholesterol/analogs & derivatives , Cholesterol/metabolism , Gastric Mucosa/drug effects , Gastric Mucosa/enzymology , Gastric Mucosa/pathology , Gene Editing , Humans , Hydroxycholesterols/metabolism , Methylcholanthrene/administration & dosage , Mice , Mice, Knockout , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Signal Transduction , Stomach Neoplasms/chemically induced , Stomach Neoplasms/enzymology , Stomach Neoplasms/mortality , Sulfotransferases/antagonists & inhibitors , Sulfotransferases/deficiency , Survival Analysis
6.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1864(10): 1293-1304, 2019 10.
Article in English | MEDLINE | ID: mdl-31170503

ABSTRACT

BACKGROUND: Bile acids (BAs) participate in lipid absorption and serve as metabolic regulatory factors in gut-liver communication. To date, there are no studies on the systemic patterns of BAs in the serum, liver, and gut in the same non-alcoholic fatty liver disease (NAFLD) model. METHODS: A targeted metabolomics approach and 16S rRNA sequencing were used to identify the profile of BAs and connection between BAs and microbiota. The role and mechanism of altered BAs on hepatic steatosis were investigated. FINDINGS: In the liver, the composition of taurocholic acid (TCA) was increased, but taurohyodeoxycholic acid (THDCA) and ursodeoxycholic acid (UDCA) were decreased. In the gut, the deconjugated form of TCA (cholic acid (CA)) was increased, while the deconjugated forms of THDCA (α-hyodeoxycholic acid (HDCA)) and ω-muricholic acid (ωMCA) were decreased. In the serum, the composition of TCA was increased, while both HDCA and THDCA were decreased. THDCA induced the gene expression of apolipoprotein, bile secretion-related proteins, and cytochrome P450 family but suppressed inflammatory response genes expression in steatotic hepatocytes by RNAseq analysis. THDCA ameliorated neutral lipid accumulation and improved insulin sensitivity in primary rat hepatocytes. The decreased HDCA level correlated with the level of Bacteroidetes, while the level of CA correlated with the levels of Firmicutes and Verrucomicrobia but correlated inversely with Bacteroidetes. CONCLUSION: BAs profiles in the serum, liver and caecal content were altered in a rat NAFLD model, which may affect hepatic lipid accumulation and correlate with gut dysbiosis.


Subject(s)
Bile Acids and Salts/metabolism , Cecum/metabolism , Fatty Liver/metabolism , Liver/metabolism , Animals , Bile Acids and Salts/blood , Cells, Cultured , Diet, High-Fat , Fatty Liver/blood , Gastrointestinal Microbiome , Male , Rats, Sprague-Dawley
7.
Cell Mol Life Sci ; 76(1): 129-145, 2019 Jan.
Article in English | MEDLINE | ID: mdl-30151693

ABSTRACT

As an analgesic and antipyretic drug, acetaminophen (APAP) is commonly used and known to be safe at therapeutic doses. In many countries, the overuse of APAP provokes acute liver injury and even liver failure. APAP-induced liver injury (AILI) is the most used experimental model of drug-induced liver injury (DILI). Here, we have demonstrated elevated levels of growth arrest and DNA damage-inducible 45α (GADD45α) in the livers of patients with DILI/AILI, in APAP-injured mouse livers and in APAP-treated hepatocytes. GADD45α exhibited a protective effect against APAP-induced liver injury and alleviated the accumulation of small lipid droplets in vitro and in vivo. We found that GADD45α promoted the activation of AMP-activated protein kinase α and induced fatty acid beta-oxidation, tricarboxylic acid cycle (TCA) and glycogenolysis-related gene expression after APAP exposure. Liquid chromatography-mass spectrometry (LC-MS) analysis showed that GADD45α increased the levels of TCA cycle metabolites. Co-immunoprecipitation analysis showed that Ppp2cb, a catalytic subunit of protein phosphatase 2A, could interact directly with GADD45α. Our results indicate that hepatocyte GADD45α might represent a therapeutic target to prevent and rescue liver injury caused by APAP.


Subject(s)
AMP-Activated Protein Kinases/metabolism , Acetaminophen/adverse effects , Antipyretics/adverse effects , Cell Cycle Proteins/metabolism , Chemical and Drug Induced Liver Injury/metabolism , Liver/drug effects , Nuclear Proteins/metabolism , AMP-Activated Protein Kinases/analysis , Analgesics, Non-Narcotic/adverse effects , Animals , Cell Cycle Proteins/analysis , Cells, Cultured , Chemical and Drug Induced Liver Injury/pathology , Citric Acid Cycle/drug effects , Enzyme Activation/drug effects , Fatty Acids/metabolism , Hepatocytes/drug effects , Hepatocytes/metabolism , Hepatocytes/pathology , Humans , Liver/metabolism , Liver/pathology , Male , Mice , Mice, Inbred C57BL , Nuclear Proteins/analysis , Signal Transduction/drug effects
8.
Biochem Biophys Res Commun ; 504(4): 892-898, 2018 10 12.
Article in English | MEDLINE | ID: mdl-30224060

ABSTRACT

Gastric cancer (GC) is one of the most common cancers and is the second-leading cause of cancer-associated morbidity worldwide. Oxysterols are oxidized derivatives of cholesterol that may be important in many biological processes, but the levels and roles of oxysterols in gastric tumours remain to be elucidated. The levels of cholesterol, oxysterols and sulfated oxysterols in human gastric tumour tissues, adjacent normal mucosal tissues, cancerous gastric juice and gastric juice obtained from healthy subjects were detected by LC-MS. It was found that the levels of 24(R/S),25-EC and 27HC in human gastric tumour tissues and cancerous gastric juice were significantly increased compared with those of adjacent normal mucosal tissues and gastric juice from healthy subjects. Compared with normal gastric mucosal tissue, the levels of sulfated 25-hydroxycholesterol (25HC3S) and the ratio of 25HC3S/25HC were decreased in human gastric tumour tissues, which might be related to the dramatically decreased SULT2A1 expression in gastric tumour tissue. Both 24(R/S),25-EC and 27HC suppressed gastric cancer proliferation, which was not altered by LXRα-siRNA treatment. The suppression of cell proliferation induced by 27HC was attenuated by LXRß-siRNA, but the suppression of cell proliferation induced by 24(R/S),25-EC was intensified by LXRß-siRNA. Both 24(R/S),25-EC and 27HC dramatically inhibited HGC-27 cell migration, which was attenuated by the co-transfection of cells with LXRα-siRNA and LXRß-siRNA, but not LXRα-siRNA or LXRß-siRNA alone. In conclusion, the accumulated 24(R/S),25-EC and 27HC in human gastric tumour tissues might play important roles in gastric cancer development.


Subject(s)
Cholesterol/analogs & derivatives , Hydroxycholesterols/metabolism , Stomach Neoplasms/metabolism , Stomach Neoplasms/pathology , Case-Control Studies , Cell Movement/drug effects , Cell Proliferation/drug effects , Cholesterol/metabolism , Cholesterol/pharmacology , Gastric Juice/metabolism , Gastric Mucosa/metabolism , Gastric Mucosa/pathology , Gene Expression Profiling , Humans , Hydroxycholesterols/pharmacology , Liver X Receptors/genetics , Liver X Receptors/metabolism , Oxysterols/metabolism , Stomach Neoplasms/drug therapy , Stomach Neoplasms/genetics , Sulfotransferases/genetics , Sulfotransferases/metabolism , Up-Regulation
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