Comparison of CT and MRI in the Diagnosis of Liver Tumors: A Retrospective Case-Control Analysis.

OBJECTIVE: To compare the diagnostic value of multi-slice computed tomography (CT) and magnetic resonance imaging (MRI) in liver tumors. METHODS: Retrospective selection of CT and MRI imaging data from 109 cases of liver tumors treated in our hospital from January 2020 to March 2023. The selection was determined through pathological examination. RESULTS: According to the pathological examination results, 61 cases were benign tumors, and 48 cases were malignant tumors. The hepatic portal flow (HPF), hepatic artery perfusion index (HPI) and hepatic artery perfusion (HAF) of malignant tumors were significantly lower than in benign tumors (P<0.05). The signal enhancement ratio of malignant tumors was significantly higher than in benign tumors, and the peak time was significantly lower than in benign tumors (P<0.05). The sensitivity (97.92%) and accuracy (97.25%) of the combined examination were significantly higher than those of MRI (83.33%, 90.83%) or CT alone (81.25%, 88.99%) (P<0.05). CONCLUSION: CT and MRI have high application value in the diagnosis and evaluation of liver tumors, and the combination of these two methods can further improve diagnostic sensitivity and accuracy, providing an objective reference for early diagnosis and treatment of liver cancer.

Ultrasound-Responsive Aligned Piezoelectric Nanofibers Derived Hydrogel Conduits for Peripheral Nerve Regeneration.

Nerve guidance conduits (NGCs) are considered as promising treatment strategy and frontier trend for peripheral nerve regeneration, while their therapeutic outcomes are limited by the lack of controllable drug delivery and available physicochemical cues. Herein, novel aligned piezoelectric nanofibers derived hydrogel NGCs with ultrasound (US)-triggered electrical stimulation (ES) and controllable drug release for repairing peripheral nerve injury are proposed. The inner layer of the NGCs is the barium titanate piezoelectric nanoparticles (BTNPs)-doped polyvinylidene fluoride-trifluoroethylene [BTNPs/P(VDF-TrFE)] electrospinning nanofibers with improved piezoelectricity and aligned orientation. The outer side of the NGCs is the thermoresponsive poly(N-isopropylacrylamide) hybrid hydrogel with bioactive drug encapsulation. Such NGCs can not only induce neuronal-oriented extension and promote neurite outgrowth with US-triggered wireless ES, but also realize the controllable nerve growth factor release with the hydrogel shrinkage under US-triggered heating. Thus, the NGC can positively accelerate the functional recovery and nerve axonal regeneration of rat models with long sciatic nerve defects. It is believed that the proposed US-responsive aligned piezoelectric nanofibers derived hydrogel NGCs will find important applications in clinic neural tissue engineering.

Molecular-Level Kinetic Model for Light Hydrocarbon Steam Cracking Based on the SU-BEM Framework.

In this work, a molecular-level kinetic model of ethane/propane steam cracking was developed by using a hybrid structural unit-bond electron matrix framework. The molecular-level simulation was conducted, creating a detailed feedstock composition, formulating the reaction rules, and automating the generation and visualization of reaction networks. Ordinary differential equations were automatically generated based on the Arrhenius equation, while the kinetic parameters were reduced via linear free energy relations (LFERs). Furthermore, proper mathematical models for mass transfer, heat transfer, and momentum transfer within the cracking furnace were integrated into the molecular-level kinetic model, enabling the simultaneous calculation of the transfer process and chemical kinetics in steam cracking. The model was validated by its precise prediction of product yields, outlet pressure, and outlet temperature, which were collected from an industrial gas-cracking furnace.

Magnetic Nanoagent Coated with an Activated Macrophage Membrane for Colorimetric Detection of Bacteria.

The construction of cell mimics replicating the surface landscape and biological functions of the cell membrane offers promising prospects for biomedical research and applications. Inspired by the inherent recognition capability of immune cells toward pathogens, we have fabricated activated macrophage membrane-coated magnetic silicon nanoparticles (aM-MSNPs) in this work as an isolation and recognition tool for enhanced bacterial analysis. Specifically, the natural protein receptors on the activated macrophage membrane endow the MSNPs with a broad-spectrum binding capacity to different pathogen species. By further incorporation of a tyramide amplification strategy, direct naked-eye analysis of specific bacteria with a detection limit of 10 CFU/mL can be achieved. Moreover, application to the diagnosis of urinary tract infections has also been validated, and positive samples spiked with bacteria can be clearly distinguished with an accuracy of 100%. This work may enrich cell membrane-based architectures and provide an experimental paradigm for point-of-care testing (POCT) detection of bacteria.

Electroacoustic Responsive Cochlea-on-a-Chip.

Organ-on-chips can highly simulate the complex physiological functions of organs, exhibiting broad application prospects in developmental research, disease simulation, as well as new drug research and development. However, there is still less concern about effectively constructing cochlea-on-chips. Here, a novel cochlear organoids-integrated conductive hydrogel biohybrid system with cochlear implant electroacoustic stimulation (EAS) for cochlea-on-a-chip construction and high-throughput drug screening, is presented. Benefiting from the superior biocompatibility and electrical property of conductive hydrogel, together with cochlear implant EAS, the inner ear progenitor cells can proliferate and spontaneously shape into spheres, finally forming cochlear organoids with good cell viability and structurally mature hair cells. By incorporating these progenitor cells-encapsulated hydrogels into a microfluidic-based cochlea-on-a-chip with culture chambers and a concentration gradient generator, a dynamic and high-throughput evaluation of inner ear disease-related drugs is demonstrated. These results indicate that the proposed cochlea-on-a-chip platform has great application potential in organoid cultivation and deafness drug evaluation.

Preferential deposition of buoyant small microplastics in surface sediments of the Three Gorges Reservoir, China: Insights from biomineralization.

Sediments act as sinks of microplastics (MPs) derived from terrestrial ecosystems. However, the fate and transport of MPs at the zone of sediment-overlying water in reservoir environment are poorly understood. Here, the MPs distribution patterns in surface sediments of the Three Gorges Reservoir (TGR) and dominant mechanisms responsible for the sinking of MPs at the zone of sediment-overlying water were comprehensively investigated. The predominant occurrence of small microplastics (<300 µm, SMPs) in surface sediments of the TGR was found, with buoyant polyethene (PE) was dominant polymer types. Interestingly, the high abundance of SMPs in sediments correlated well with the Ca2+/Mg2+ in overlying water, suggesting that divalent cations in overlying water may enhance the preferential deposition of SMPs. Simulation sinking experiments under the presence of Microcystis aeruginosa and two divalent cations using different-sized PE MPs demonstrated that the greater deposition of SMPs was mainly the result of the formation of biogenic calcite on the surface of MPs rather than magnesium minerals, which provides stronger ballasting effects for SMPs than for large MPs. This study first highlights that the impact of biomineralization on preferential sinking of SMPs and enhances the understanding of the transport behaviour of MPs in aquatic environment.

Geochemical characteristics and environmental implication of rare earth elements in sediments from the Three Gorges Reservoir, China.

The construction and operation of the Three Gorges Dam occluded sediment transportation in the Yangtze River. However, the sources, transport processes, and environmental impacts of these sediments on the Three Gorges Reservoir (TGR) remain unclear. Here, we used rare earth elements (REEs) to trace the transport pathways of sediments in the TGR, China. Geochemical characteristics including the chemical composition and fractionation, temporal and spatial distribution, and potential sources of REEs were also evaluated in this study. The individual REEs concentration in the TGR sediments followed the Oddo-Harkins rule, with the mean REEs value of 207.33 µg/g. REEs concentrations in the midstream were higher than those in the upstream and downstream of the TGR. Statistical analysis showed that water impoundment phase had no significant influence on the distribution of REEs. TGR sediments are mainly derived from terrigenous detrital particulates, characterized by a distinctive enrichment in light REEs, with its percentage higher than 90 % of the total REEs. The significant positive correlation among the REEs confirmed that they are co-existed and shared the similar sources. Multiple provenance analysis approaches using discriminant function analyses, provenance indices, and La/Yb-La/Sm-Gd/Yb ternary diagrams further indicated that the REEs in sediments originated from the weathering of mudstone in the basin of TGR. After periodic water level fluctuation for more than six years, the chemical compositions of REEs in TGR sediments slightly differed from those of the Yangtze River sediments before TGR construction, but were similar to those of the downstream of the Yangtze River. Therefore, this study indicated that the construction and operation of the TGR changed the chemical compositions and the origin of the sediments in the Yangtze River, which can provide useful insights into the transport pathways of TGR sediments and their impacts on the fluvial environment.

A comprehensive study on the co-removal of Cr (VI) and ciprofloxacin via microbial-photocatalytic coupling: Mechanistic insights and performance evaluation.

The increasing contamination of water systems by antibiotics and heavy metals has become a growing concern. The intimately coupled photocatalysis and biodegradation (ICPB) approach offers a promising strategy for the effective removal of mixed pollutants. Despite some prior research on ICPB applications, the mechanism by which ICPB eliminates mixed pollutants remains unclear. In our current study, the ICPB approach achieved approximately 1.53 times the degradation rate of ciprofloxacin (CIP) and roughly 1.82 times the reduction rate of Cr (VI) compared to photocatalysis. Remarkably, after 30 days, the ICPB achieved a 96.1% CIP removal rate, and a 97.8% reduction in Cr (VI). Our investigation utilized three-dimensional fluorescence analysis and photo-electrochemical characterization to unveil the synergistic effects of photocatalysis and biodegradation in removal of CIP and Cr (VI). Incorporation of B-Bi3O4Cl (B-BOC) photocatalyst facilitated electron-hole separation, leading to production of ·O2-, ·OH, and h+ species which interacted with CIP, while electrons reduced Cr (VI). Subsequently, the photocatalytic products were biodegraded by a protective biofilm. Furthermore, we observed that CIP, acting as an electron donor, promoted the reduction of Cr (VI). The microbial communities revealed that the number of bacteria favoring pollutant removal increased during ICPB operation, leading to a significant enhancement in performance.

A Biomimetic Cardiac Fibrosis-on-a-Chip as a Visible Disease Model for Evaluating Mesenchymal Stem Cell-Derived Exosome Therapy.

Cardiac fibrosis acts as a serious worldwide health issue due to its prevalence in numerous forms of cardiac disease and its essential link to cardiac failure. Considering the efficiency of stem cell therapy for cardiac fibrosis, great efforts have been dedicated to developing accurate models for investigating their underlying therapeutic mechanisms. Herein we present an elaborate biomimetic cardiac fibrosis-on-a-chip based on Janus structural color film (SCF) to provide microphysiological visuals for stem cell therapeutic studies. By coculturing cardiomyocytes (CMs) and cardiac fibroblasts (FBs) on Janus SCF with fibrosis induction, the chip can recreate physiological intercellular crosstalk within the fibrotic microenvironment, elucidating the physiological alterations of fibrotic hearts. In particular, the Janus structural color film possesses superior perceptual capabilities for capturing and responding to a weak cardiac force, demonstrating synchronized structural color shifts. Based on these features, we have not only explored the dynamic relationship between color mapping and the evaluated disease phenotype but also demonstrated the self-reporting capacity of the cardiac fibrosis-on-a-chip for the assessment of mesenchymal stem cell-derived exosome therapy. These features suggest that such a chip can potentially facilitate the evolution of precision medicine strategies and create a protocol for preclinical cardiac drug screening.

Dual function of magnetic field in enhancing antibiotic wastewater treatment by an integrated photocatalysis and fluidized bed biofilm reactor (FBBR).

The integrated photocatalysis and fluidized bed biofilm reactor (FBBR) is an attractive wastewater treatment technique for managing wastewater containing antibiotics. However, the fast recombination of photoinduced charge and low microbial activity limit the degradation and mineralization efficiency for antibiotics. To address this, we attempt to introduce magnetic field (MF) to the integrated system with B-doped Bi3O4Cl as the photocatalysts to effectively improve removal and mineralization of ciprofloxacin (CIP). As a consequence, the degradation rate reaches 96% after 40 d in integrated system with MF. The biofilm inside the integrated system with MF carrier can mineralize the photocatalytic products, thereby increasing the total organic carbon (TOC) degradation rate by more than 32%. The electrochemical experiment indicates the Lorentz force generated by MF can accelerate charge separation, increasing the electron concentration. Simultaneously, the increased amounts of electrons lead to the generation of more ·OH and ·O2-. MF addition also results in increased biomass, increased biological respiratory activity, microbial community evolution and accelerated microbial metabolism, enabling more members to biodegrade photocatalytic intermediates. Therefore, applied MF is an efficient method to enhance CIP degradation and mineralization by the integrated system.

Effects of lysed sludge reflux point on ultrasound lysis-cryptic growth in anaerobic/aerobic (A/O) wastewater treatment: Sludge reduction, microbial community, and metabolism.

Ultrasonication allows sludge reduction to be performed in situ during wastewater treatment, and the reflux point of the lysed sludge affects this performance. This study investigated the effects of reflux point (anaerobic stage, carbon/nitrogen (C/N) lowest stage, and aerobic stage) on sludge lysis-cryptic growth in an anaerobic/aerobic reactor and variations in the sludge and microbial community. The best reflux point occurred at the lowest C/N ratio stage, and a 50.96% reduction in excess sludge was achieved. The reflux of the lysed sludge to the aerobic stage reduced nitrogen and phosphorus removal. The reflux of the lysed sludge decreased the average sludge size, reaching 29.2 µm when reflux to the aerobic stage. Scanning electron microscopy showed that the sludge surface was unaffected by the reflux point. The Fourier-transform infrared spectrometry and X-ray photoelectron spectroscopy results showed that the most prominent variation in the intensity of the sludge functional groups occurred when the reflux was at the lowest C/N stage. The amount of extracellular polymeric substances decreased the most during reflux to the anaerobic stage. The sludge microbial communities varied with the reflux point, and the dominant phyla during reflux to the anaerobic, lowest C/N, and aerobic stages were Bacteroidetes, Firmicutes, and Bacteroidetes, respectively. Furthermore, the reflux point did not alter the metabolic pathway of sludge microorganisms but increased the number of enzymes in metabolic pathways.

Tailoring biomaterials for biomimetic organs-on-chips.

Organs-on-chips are microengineered microfluidic living cell culture devices with continuously perfused chambers penetrating to cells. By mimicking the biological features of the multicellular constructions, interactions among organs, vascular perfusion, physicochemical microenvironments, and so on, these devices are imparted with some key pathophysiological function levels of living organs that are difficult to be achieved in conventional 2D or 3D culture systems. In this technology, biomaterials are extremely important because they affect the microstructures and functionalities of the organ cells and the development of the organs-on-chip functions. Thus, herein, we provide an overview on the advances of biomaterials for the construction of organs-on-chips. After introducing the general components, structures, and fabrication techniques of the biomaterials, we focus on the studies of the functions and applications of these biomaterials in the organs-on-chips systems. Applications of the biomaterial-based organs-on-chips as alternative animal models for pharmaceutical, chemical, and environmental tests are described and highlighted. The prospects for exciting future directions and the challenges of biomaterials for realizing the further functionalization of organs-on-chips are also presented.

Wet Deposition of Globally Transportable Microplastics (<25 µm) Hovering over the Megacity of Beijing.

Microplastics of size <25 µm possess globally transportable features, but the impact of precipitation on their transport remains unclear. Here, microplastics were detected in all 10 studied rainfalls in Beijing, with <25 µm microplastics present in 8 rainfalls. Interestingly, microplastic abundance (7590-136,778 items·m-3) was tentatively linked to maximum rainfall intensity, with <25 µm microplastics making up 39.6 (±27.5)% of the total count. The composition of <25 µm microplastics differed from that of larger microplastics, although both mainly comprised polystyrene, polyethylene, and polypropylene. The microplastic communities differed among rainfalls, suggesting that atmospheric transport is a highly dynamic process. The first rainfall exhibited the highest microplastic abundance and community diversity after long-term exposure to dry atmospheric environment. The deposited microplastics were unstable and highly fragmented according to the conditional fragmentation model. The wet deposition rate of the microplastics was calculated as 2-463 µg·m-2 (146-8629 items·m-2) per rain, amounting to 25.44 tons per annum in Beijing. Although <25 µm microplastics represented a negligible proportion (0.00-1.24%) of the overall mass load of microplastics, their numerical abundance was high. Our results demonstrate that precipitation is an effective mechanism for removing airborne microplastics, which may enter urban soils and waters, exacerbate microplastic burdens in the environment, and cause potential risk for human health.

Living Anisotropic Structural Color Hydrogels for Cardiotoxicity Screening.

Environmental toxins can result in serious and fatal damage in the human heart, while the development of a viable stratagem for assessing the effects of environmental toxins on human cardiac tissue is still a challenge. Herein, we present a heart-on-a-chip based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) cultured living anisotropic structural color hydrogels for cardiotoxicity screening. Such anisotropic structural color hydrogels with a conductive parallel carbon nanotube (CNT) upper layer, gelatin methacryloyl (GelMA) interlayer, and inverse opal bottom layer were fabricated by a sandwich replicating approach. The inverse opal structure endowed the anisotropic hydrogels with stable structural color property, while the parallel and conductive CNTs could induce the hiPSC-CMs to grow in a directional manner with consistent autonomous beating. Notably, the resultant hiPSC-CM-cultured hydrogel exhibited synchronous shifts in structural color, responding to contraction and relaxation of hiPSC-CMs, offering a visual platform for monitoring cell activity. Given these features, the hiPSC-CM-cultured living anisotropic structural color hydrogels were integrated into a heart-on-a-chip, which provided a superior cardiotoxicity screening platform for environmental toxins.

Using optimized particle imaging of micro-Raman to characterize microplastics in water samples.

Characterizing the chemical properties, morphologies, size, and quantities of microplastics (MPs) in water samples with high precision is critically important for understanding the environmental behaviors of MPs. Traditional detection methods, such as Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy point-by-point detection, provide worthy reference techniques but are time- and labor-consuming. We established a super time-saving and high-precision technique to characterize MPs using micro-Raman automatic particle identification (MR-API). Based on the identification of PS spheres, screen magnification, exposure time, and the number of scans are selected as crucial detection parameters for MR-API analysis, which highly affect the precision of the results. Detecting particles down to 1 µm requires magnification of the mosaic until the scale showed 200 µm. The recommended setting parameters were 83.33 or 100 ms exposure time, 20 scans, 7 mW laser power, and 1 µm image pixel size, suitable for polystyrene (PS), polypropylene (PP), polyethylene terephthalate (PET), polyethylene (PE), polyvinyl chloride (PVC), and polyamide (PA) particles detection. With the complete procedure of MR-API measurements, the recovery of MPs was 61.67-90.00 %. To validate the feasibility of the MR-API, the method was used to detect samples of known plastic types (mask leachates) and unknown plastic types (urban lake). A total of 4540 particles in the sample of mask leachates consuming 35 h 50 min 43 s, and 0.92 ± 0.49 % of particles were identified as MPs. The urban river sample efficiently identified PP, PET, PE, PVC, PS, EVA, and VC/VAC MPs using this method. The detected MPs size ranged from 8.3 to 5000 µm, saving 75.03 % and 58.38 % of the time compared to the conventional micro-FTIR and micro-Raman point-by-point methods, respectively. Therefore, this method is effective for detecting MPs in the environmental samples and has excellent prospects.

Melanin-Integrated Structural Color Hybrid Hydrogels for Wound Healing.

Hydrogel patches have outstanding values in wound treatment; challenges in this field are concentrated on developing functional and intelligent hydrogel patches with new antibacterial strategies for improving healing process. Herein, a novel melanin-integrated structural color hybrid hydrogel patches for wound healing is presented. Such hybrid hydrogel patches are fabricated by infusing asiatic acid (AA)-loaded low melting-point agarose (AG) pregel into the melanin nanoparticles (MNPs)-integrated fish gelatin inverse opal film. In this system, MNPs not only impart the hybrid hydrogels with properties of photothermal antibacterial and antioxidant, but also improve the visibility of structural colors by providing an inherent dark background. Besides, the photothermal effect of MNPs under near-infrared irradiation can also trigger liquid transformation of AG component in hybrid patch, resulting in the controllable release of its loaded proangiogenic AA. Attracting, this drug release induced refractive index variations in the patch can be detected as visible structural color shifting, which can be used to monitor their delivery processes. Benefiting from these features, the hybrid hydrogel patches are demonstrated to achieve excellent therapeutic effects for in vivo wound treatment. Thus, it is believed that the proposed melanin-integrated structural color hybrid hydrogels are valuable as multifunctional patches for clinical applications.

Tailoring Functional Micromotors for Sensing.

Micromotors are identified as a promising candidate in the field of sensing benefiting from their capacity of autonomous movement. Here, a review on the development of tailoring micromotors for sensing is presented, covering from their propulsion mechanisms and sensing strategies to applications. First, we concisely summarize the propulsion mechanism of micromotors involving fuel-based propulsion and fuel-free propulsion introducing their principles. Then, emphasis is laid to the sensing stratagems of the micromotors including speed-based sensing strategy, fluorescence-based sensing strategy, and other strategies. We listed typical examples of different sensing stratagems. After that, we introduce the applications of micromotors in sensing fields including environmental science, food safety, and biomedical fields. Finally, we discuss the challenges and prospects of the micromotors tailored for sensing. We believe that this comprehensive review can help readers to catch the research frontiers in the field of sensing and thus to burst out new ideas.

Geochemical characteristics and ecotoxicological risk of arsenic in water-level-fluctuation zone soils of the Three Gorges Reservoir, China.

The Three Gorges Reservoir (TGR) has formed the water-level-fluctuation zone (WLFZ) due to reservoir regulation. However, as a sensitive zone in reservoir, little is known about the geochemical process and ecotoxicological risk of arsenic (As) in WLFZ soils under the anti-seasonal flow regulation. Hence, the anthropogenic contamination, mobility and ecotoxicological risks of As in WLFZ soils of the TGR were comprehensively assessed using the geochemical baseline concentration (GBC), chemical fractions, diffusive gradients in thin films (DGT) and toxicity data. The As concentrations in WLFZ soils showed a trend of increasing at the early stage of water impoundment and then stabilizing in recent years, which presented a low ecological risk of As according to the assessment by pollution indices. Based on GBC calculations, the average anthropogenic contribution of As was 13.95 %, indicating a slight influence of human activities. The distribution of labile As measured by DGT in WLFZ soils was mainly controlled by the Fe/Mn oxides, pH and organic matter. The DGT-induced fluxes in soils (DIFS) model further implied that resupply of As to soil solution was partially sustained by the soil solid phase, in which the resupply capacity was low and limited by the adsorption and desorption kinetics. In addition, the DGT was combined with toxicity data to obtain the risk quotient (RQ) and probabilistic risk assessment. The RQ value was lower than 1, indicating a low toxicity risk in WLFZ soils. Furthermore, the As in WLFZ soils had a low probability (5.97E-3 % and 7.77E-2 % in the mainstream and tributary, respectively) of toxic effects toward the aquatic biota. This study provides a comprehensive evaluation for the mobility and toxicity risk of As in WLFZ soils, which is beneficial to the prevention and control of heavy metals pollution in the riparian soils of lakes and reservoirs.

Bioinspired optical and electrical dual-responsive heart-on-a-chip for hormone testing.

Heart-on-chips have emerged as a powerful tool to promote the paradigm innovation in cardiac pathological research and drug development. Attempts are focused on improving microphysiological visuals, enhancing bionic characteristics, as well as expanding their biomedical applications. Herein, inspired by the bright feathers of peacock, we present a novel optical and electrical dual-responsive heart-on-a-chip based on cardiomyocytes hybrid bright MXene structural color hydrogels for hormone toxicity evaluation. Such hydrogels with inverse opal nanostructure are generated by using pregel to replicate MXene-decorated colloidal photonic crystal (PhC) array templates. The attendant MXene in the hydrogels could not only enhance the saturation of structural color, but also ensure the composite hydrogel with excellent electroconductivity to facilitate the synergetic beating of their surface cultured cardiomyocytes. In this case, the hydrogels would undergo a synchronous deformation and generate shift in corresponding photonic band gap and structural color, which could be employed as visual signal for self-reporting of the cardiomyocyte mechanics. Based on these features, we demonstrated the practical value of the optical and electrical dual-responsive structural color MXene hydrogels constructed heart-on-a-chip in hormone toxicity testing. These results indicated that the proposed heart-on-a-chip might find broad prospects in drug screening, biological research, and so on.

Vertical Differentiation of Microplastics Influenced by Thermal Stratification in a Deep Reservoir.

Microplastics (MPs) are an emerging environmental concern. However, vertical transport of MPs remains unclear, particularly in deep reservoirs with thermal stratification (TS). In this study, the vertical variation in MP organization, stability, migration, and the driving factors of the profile in a deep reservoir were comprehensively explored. This is the first observation that TS interfaces in a deep reservoir act as a buffer area to retard MP subsidence, especially at the interface between the epilimnion and the metalimnion. Interestingly, there was a size-selection phenomenon for MP sinking. In particular, the high accumulation of large-sized MPs (LMPs; >300 µm) indicated that LMPs were more susceptible to dramatic changes in water density at the TS interfaces. Furthermore, simultaneous analysis of water parameters and MP surface characteristics showed that the drivers of MP deposition were biological to abiotic transitions during different layers, which were influenced by algae and metals. Specifically, scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy and microscopic Fourier transform infrared analyses implied that the occurrence of metals on the MP surface can promote MP deposition in the hypolimnion. Our findings demonstrated that TS significantly influenced the MP fate in deep reservoirs, and the hotspot of MP exposure risk for vulnerable benthic organisms on the reservoir floor deserves more attention.