ABSTRACT
OBJECTIVE: To study immunoregulation effects on normal pulpal cells of the mucopolysaccharides MP1 and MP2 extracted from Asterias rollestoni body wall and viscera, respectively. METHODS: The orthogonal design was employed to obtain the best possible combination of the critical parameters for mucopolysaccharide MP2. MP1, MP2, glycosides, MP1 + glycosides and MP2 + glycosides were added respectively into pulpal cells culture media to test their effects on spleen cells proliferations by MTT assay. RESULTS: MP1 and MP2 could certainly increase the proliferation of the normal splenocyte and inhibit glycosides cytotoxicity. Compared with controls, MP2 reversed the cytotoxicity of glycosides at concentration of 25 to 40 mg/mL, the greatest activity appearing at the dose of 40 mg/mL (P<0.01), while MP1 didn't reverse it. CONCLUSION: MP1 and MP2 can enhance immunity of normal mice, and MP2 can significantly reverses the cytotoxicity of glycosides in vitro.
